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《Journal of Anhui Science and Technology University》 2007-05
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Comparative Effects among Different Inoculating Methods with Sharp Eyespot Fungus(Rhizoctonia cerealis) in Wheat

YAN Wei,CAI Shi-bin,WU Ji-zhong,ZHANG Xian-yi,WU Xiao-you (Insititute of Food Crops,Jiangsu Academy of Agricultural Science,Nanjing 210014,China)  
To identify the usability of the different inoculation methods in genetics research on resistance to sharp eyespot,three inoculation methods,i.e.applying inoculums in sowing-pitch when seeding(sowing-pitch method),inserting affected toothpicks between sheath and stem at booting(toothpick method),and applying inoculums on soil surface in early spring(soil surface method),were compared for their effectiveness in infection and development of sharp eyespot in wheat.The results showed that there were significant difference among inoculation methods and genotypes.Effects of sowing-pitch method were more variable between replications and could only be used in preliminary large-scale screening as it is easy to hand.Toothpick method could induce the highest disease incidents with highly repeatable results.It could be used in genetic studies and small-scale evaluation as this method is more labors.Soil surface method combined advantages of toothpick method and sowing-pitch method and was the handiest and most effective method to evaluate wheat sharp eyespot.There were significant differences in disease incident among genotypes.SW038,WHO6-7,Niavt14 and CI12633 showed resistance and could be used as resistant parents in wheat breeding.
【Fund】: 国家“十五”攻关项目(2004BA525B03);; 江苏省自然科学基金资助项目(BK2004170)
【CateGory Index】: S435.121
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