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《Journal of Anhui Science and Technology University》 2007-06
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Extraction of Polysaccharide in Black Glutinous Corn and Comparison of Removing Protein Procedure

ZHANG Zhong,YANG Li(Anhui Science and Technology University,Fengyang 233100,China)  
The extraction of polysaccharide in black glutinous corn and the comparison of removing protein separation procedure were studied in this paper.The optimum procedure of removing protein was studied in two advanced methods(Protein was removing by Chloroform-n-Butyl alcohol and Tricloroacetic acid-n-Butyl alcohol) by L9(34) orthogonal experiment design.After determining the optimum preparation procedure by the percentage of removed protein,the percentage of composed polysaccharide and absorbance at 510 nm were compared in this paper.The results showed that the method of using Tricloroacetic acid-n-Butyl alcohol had a higher percentage of removing protein,which is 70.69%,but a lower percentage of composed polysaccharide,which is 67.87%.The method of using Chloroform-n-Butyl alcohol had lower percentage of removing protein and higher percentage of compoing polysaccharide,which are 51.39% and 80.43% respectively.The optimum preparation procedure established in this paper may provide a reference of extraction and separation of the polysaccharide in black glutinous corn.
【Fund】: 安徽省教育厅项目(2006KJ200B)和引进(稳定)人才专项基金(ZRC200691)
【CateGory Index】: TQ461
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