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《Journal of Anhui Science and Technology University》 2010-01
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Effects of Cold Acclimation on Cold Resistance in Plantlets of Phalaenopsis Amabilis

MA Yu-han1,CUI Guang-rong2,ZHAO Yan1,CHU Jun1,ZHOU Zheng-yi1(1.College of Life Science,Anhui Science and Technology University,Fengyang 233100,China;2.College of Botany,Anhui Science and Technology University,Fengyang 233100,China)  
To elucidate the freezing tolerance mechanism of perennial plants,Phalaenopsis amabilis Protocorm-like body was used as model.Activities of antioxidant enzyme and solute contents were investigated in plantlets of Phalaenopsis amabilis.Cold-hardening(17℃/12℃) increased the activities of SOD,CAT in plantlets.A similar increase was also observed in the protein,proline and soluble carbohydrates content.Meanwhile,Cold acclimation decreased the MDA content.These results obtained suggest that the higher activities of SOD,CAT as well as the higher protein,proline,soluble carbohydrates and MDA content may be biochemical adaptation for freezing tolerance in cold-hardened Phalaenopsis amabilis plantlets.In conclusion,17℃/12℃ is the optimal temperature to enhance the cold resistance in Plantlets of Phalaenopsis amabilis.
【Fund】: 安徽科技学院引进人才专项(ZRC2007135);; 安徽科技学院引进人才专项(ZRC2007147);; 安徽省自然科学基金项目(070411010);; 安徽省教育厅省级自然科学基金项目(KJ2007B053)
【CateGory Index】: S682.31
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