Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Science and Technology University》 2012-05
Add to Favorite Get Latest Update

Optimization of SRAP Molecular Technology and Analysis of Genetic Relationship in Stevia rebaudiana Bertoni

HU Neng-bing1,2,HE Ke-qin1,2,ZHANG Zi-xue1,2,LIN Ping1,2,SUI Yi-hu1,2,ZHOU Yu-li1,2,CUI Guang-rong1,2*(1.College of Botany,Anhui Science and Technology University,Fengyang 233100,China; 2.Anhui Province Research and Technology Center of Stevia Rebaudiana Bertoni,Bengbu,233000,China)  
Optimization of 5 factors of sequence-related amplified polymorphism(SRAP) reaction system using the orthogonal design and relationship analysis of 12 kinds of cultivars in Stevia rebaudiana Bertoni were studied.The results indicated that the factor influencing SRAP system followed the order: MgCl2,Primers,Taq,dNTPS and DNA.The optimization system of 20μL included 12.7μL of ddH20,3μL of 10×Buffer with 15mmol/L MgCl2,0.8μL of 2.5 mmol/L dNTPS,0.75μL of 10μmol/L Primers,1μL of 20ng DNA and 5 unit of Taq polymerase.The result of clustering showed that the genetic distance among 12 cultivars ranged from 0.24 to 0.69,and could be classified into four groups when the genetic distance was 0.48.
【Key Words】: Stevia rebaudiana Bertoni Sequence-related amplified polymorphism Optimization Genetic realtionship
【Fund】: 安徽省教育厅省级重点自然科学基金项目(KJ2010A073);; 蚌埠市科技计划项目(科字051);; 安徽科技学院青年基金项目(ZRC2011281)
【CateGory Index】: S566.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 XUE Dan-dan1,2,ZHENG Yi-qi2,WANG Zhi-yong2,GUO Hai-lin2,CHEN Xuan2,LIU Jian-xiu2(1.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China);Establishment and optimization of SRAP-PCR reaction system for Zoysia[J];Acta Prataculturae Sinica;2008-06
2 SUN Zhen-jiu~1,WANG Ya-juan~2,ZHANG Xian~2(1 Tianjin Agricultural College,Tianjin 300384,China;2 Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Progress in Molecular Marker-Assisted Breeding of Cucumber[J];Acta Botanica Boreali-Occidentalia Sinica;2006-06
3 Ren Yu Wang Deyuan Zhang Yindong Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou , 510640 State Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, 571101;Progresses in the Heterosis Molecular Prediction in the Crops[J];Molecular Plant Breeding;2004-03
4 WANG Zhen-guo1,2,ZHANG Hai-ying2,YU Guang-jian1,ZHANG Feng2,WANG Yong-jian2,XU Yong2,GUO Shao-gui2,GONG Guo-yi2(1.College of Horticulture,Northeast Agricultural University,Haerbin 150030,China;2.Beijing Vegetable Research Center,Beijing 100097,China);The Orthogonal Optimization of SRAP Amplification System in Cucumber[J];Acta Agriculturae Boreali-Sinica;2007-04
5 WANG Kai,WEI Shan-zhong,LUO Jiang et al.;DNA Molecular Markers and Their Advances[J];Journal of Heilongjiang August First Land Reclamation University;2003-01
6 HU Nengbing,HE Keqin,ZHANG Zixue,LIN Ping,SUI Yihu,CUI Guangrong~* (1.College of Plant Science,Anhui Science and Technology University,Fengyang 233100,Anhui,China; 2.Anhui Province Research and Technology Center of Stevia rebaudiana Bertoni,Bengbu 233000,Anhui,China);Optimization of'RAPD Reaction System in Stevia rebaudiana Bertoni[J];Acta Laser Biology Sinica;2011-04
7 LI Xue-lin~1,LIANG Hua~2,ZHANG Ze-min~1,ZHENG Yue-jin~1,ZHANG Fu-hou~1(1.Agricultural College,Henan University of Science and Technology,Luoyang471003,China;2.Department of Food & Bioengineering,Henan University of Science and Technology,Luoyang 471003,China);Application advance of molecular marking technique in plant genetics and breeding[J];Journal of Henan University of Science and Technology(Agricultrual Science);2004-02
8 WU Yingying1,PENG Fangren1,HAO Mingzhuo1,CHEN Longsheng2,CHEN Yongzhong2(1.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China; 2.Hunan Forestry Academy,Changsha 410004,China);Establishment reaction system of the SRAP-PCR in Camellia oleifera[J];Journal of Nanjing Forestry University(Natural Sciences Edition);2011-05
9 LI Li1, PENG Jian-ying1, ZHENG Bao-qiang2 (1.College of Horticulture, Agricultural University of Hebei, Baoding 071001,China; 2. Forestry Research Institute, Chinese Academy of Forestry, Beijing 100091,China);Optimization of SRAP-PCR System with Orthogonal Design in Zixiphus jujube[J];Journal of Agricultural Biotechnology;2008-02
10 LI Da,YU Xiao-ying,WU Li-ying,YAO Jue,QIU Shou,ZHOU Hong-can College of Horticulture and Landscapes,Hunan Agricultural University,Changsha 410128,China;Advance of Molecular Markers in Ornamental Plants[J];Letters in Biotechnology;2007-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Ren Jingwen et al.(Fengyang County Institute of Agricultural Science,Fengyang 233100,China);SSR Marker Technology and Its Application to Maize[J];Anhui Agricultural Science Bulletin;2011-11
2 ZHAO Xin-liang et al (Henan Institute of Science and Technology,Xinxiang,Henan 453003);Optimization of SRAP System in Maize[J];Journal of Anhui Agricultural Sciences;2006-15
3 ZHAO Xin-liang et al (Northwest A&F University,Yangling,Shaanxi 712100);Study on Classification of Maize Inbred Lines in Henan with SRAP Marker Technology[J];Journal of Anhui Agricultural Sciences;2007-07
4 DING Jin-ping et al (College of Agronomy,Guangxi University,Nanning,Guangxi 530003);Establishment and Optimization of SRAP Reaction Conditions for Arachis hypogaea L.[J];Journal of Anhui Agricultural Sciences;2008-10
5 GUO Da-long et al(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003);Establishment and Optimization of SRAP Amplification System in Bitter Melon(Momordica charantial L.)[J];Journal of Anhui Agricultural Sciences;2008-18
6 ZHANG Bian-jiang et al (Department of Life Science,Nanjing Xiaozhuang College,Nanjing,Jiangsu 211171);Development of Plant Breeding Selection Technology and Its Theoretical Basis[J];Journal of Anhui Agricultural Sciences;2008-19
7 WANG Shao-wen et al(College of Agronomy,Liaocheng University,Liaocheng,Shandong 252059);Extraction of DNA from Leaves of Aesculus chinensis[J];Journal of Anhui Agricultural Sciences;2008-32
8 LU Hang et al(College of Forestry,Sichuan Agriculture University,Ya'an,Sichuan 625014);Extraction and PCR Detection of Total DNA of Corythucha ciliate Say[J];Journal of Anhui Agricultural Sciences;2011-22
9 WANG Fang et al(Experiment Administration Center,Zhongzhou University,Zhengzhou,Henan 450044);Sequence-related Amplified Polymorphism Markers and Their Application in Plant research[J];Journal of Anhui Agricultural Sciences;2011-23
10 FAN Xiao-ning et al(Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province,College of Life Science,Anhui Normal University,Wuhu,Anhui 241000);Optimization of SSR-PCR System for C.oleifera[J];Journal of Anhui Agricultural Sciences;2011-23
China Proceedings of conference Full-text Database 10 Hits
1 SHI Qian-qian WANG Yan ZHOU Lin (Research Institute of Forestry,CAF,SFA Key of Breeding and Cultivation,Beijing 100091);Optimization of SRAP-PCR System and Selection of Primers for Paeonia suffruticosa Based on Capillary Electrophoresis[A];[C];2011
2 REN Yu YANG Guang-sui YIN Jun-mei (Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou 571737);Analysis of Genetic Diversity in Dendrobium Germplasm by SRAP Makers[A];[C];2007
3 ;SRAP Molecular Marker Related to Green Peel Gene in Cucumber[A];[C];2008
4 ;The Principle and Research Advancement of Sequence-Related Amplified Polymorphism[A];[C];2008
5 FAN Shu-li, YU Shu-xun, SONG Mei-zhen(Cotton Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, He′nan 455004,China);Construction of Molecular Marker Linkage Map in Short-season Cotton and Earliness QTLs Mapping[A];[C];2005
6 ;利用微卫星标记分析天然棕色棉的遗传多样性[A];[C];2008
7 PENG Bo1,2 Zhang Hong2 Hu Xingming2 (1College of Life Sciences,Wuhan University,Wuhan 430072,China ;2Industrial Crops Institute,Hubei Academy of Agriculture Sciencse,Wuhan 430070,China);SSR marker and its application in Mulberry Genetics and Breeding Research[A];[C];2009
8 WANG Zhi-yong~(1,2) YUAN Xue-jun~(1,2) LIU Jian-xiu~(1*) GUO Hai-lin~1 (1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China; 2.Department of Horticulture,Nanjing Agricultural Univeristy,Nanjing 210095,China);Optimization of SRAP-PCR System on Cynodon dactylon(L.) Pers.by orthongonal design and selection of primers[A];[C];2007
9 Pian Rui-qi (Laboratory for Biotechnology in Tropical and Subtropical Forest Science; College of forestry; South China Agricultural University Guangzhou 510642; Biological Institute, Beijing Forestry University Beijing 100083) Ni Hui-qun Chen Xiao-yang (Biological Institute, Beijing Forestry University Beijing 100083);Optimization of SRAP System and Genetic Relationship Analysis of Lespedeza[A];[C];2009
10 CHEN Gang FENG Jian WANG Qian-chun PAN Wen-li FAN Jun-gang (Liaoning Research Academy of Forestry Sciences Shenyang 110032);SRAP Marker and Its Prospect of the Application in Forest Tree Genetic Breeding[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 TAN Tan et al(College of Natural Conservation,Bejing Forestry University,Beijing 100083);Optimization of the ISSR-PCR Reaction System of Cyclocarya paliurus[J];Journal of Anhui Agricultural Sciences;2006-24
2 SUI Yi - hu, ZHANG Zi - xue, XING Su - zhi, LING Tong - zhong, JIN Gui - fang (Department of Agronomy,Auhui Technical Teachers College,Fengyang 233100,China);Research on Some Physiologic Indexes and Germination of Purple Chili Line YN99007 under NaCl Stress[J];Journal of Anhui Technical Teachers College;2004-06
3 SUI Yi-hu1,2,ZHANG Zi-xue1,HU Neng-bing1,WANG Xia1(1.College of Plant Science,Anhui Science and Technology University,Fengyang 233100,China;2.The State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210094,China);Changes of some Quality Indexes in the Process of Purple Chili mix-pickled with Cucumber or Mustard[J];Journal of Anhui Science and Technology University;2007-06
4 Zhou Chunling; Dai Silan. College of Landscape Architecture, Beijing For. Univ., 100083, P. R. China.;AFLP analysis of some Dendranthema spp.[J];Journal of Beijing Forestry University;2002-Z1
5 MING Jun; ZHANG Qixiang. College of Horticulture and Landscape Architecture, Yangtze University, Jingzhou, 434025, P. R. China.;AFLP DNA fingerprinting analysis of close genetic relationships of cultivars of Prunus mume Sieb. et Zucc[J];Journal of Beijing Forestry University;2004-05
6 Ming Jun (Institute of vegetables and Flowers, The Chinese Academy of Agricultural Sciences, Beijing, 100081)Zhang Qixiang (College of Landscape Architecture, Beijing Forestry University,100083,P.R. China);Paternity Analysis of Prunus mume‘Meiren’[J];Journal of Beijing Forestry University;2004-S1
7 Yang Chaodong 1,2 Zhang Junwei 1 Xiong Caifeng 3 Bao Manzhu 1 (1 College of Horticulture and Forestry Sciences, Huazhong Agricultural University, 430070,Wuhan,P.R.China 2 Xiaogan University, 432100,Xiaogan,P.R.China 3 China Mei Flower Research Center,430074,Wuhan,P.R.China);Rapid Identification of Parentage in Prunus mume by AFLP Assay[J];Journal of Beijing Forestry University;2004-S1
8 Wang Caiyun 1,2 Chen Jun-yu 1 Maarten A.Jongsma 3 (1 College of Landscape Architecture ,Beijing Forestry University, 100083,P.R.China 2 College of Horticulture and Forestry, Huazhong Agricultural University, 430070,Wuhan, P.R.China 3 Plant Research International, Wageningen University and Research Center, PO Box 16, 6700 AA Wageningen, The Netherlands);Molecular Evolution and Phylogeny of Florist's Chrysanthemum and Related Species[J];Journal of Beijing Forestry University;2004-S1
9 WANG Xian 1,2;ZHANG Qi-xiang2;YANG Qiu-sheng1; CHAI Yong-sheng3. 1 College of Forestry and Horticulture, Henan Agriculture Univerisity, Zhengzhou, 450002, P. R. China; 2 College of Landscape Architecture, Beijing Forestry University, 100083, P. R. China; 3 College of Resources and Environment, Beijing Forestry University, 100083, P. R. China.;Genetic relationship of Lagerstroemia indica by AFLP.[J];Journal of Beijing Forestry University;2005-01
10 Sun Yizhen et al (Beijing Agriculural College,Beijing 102206);Analysis to Zea Mays Sinesis Inbred Lines Combining Ability and to Apply RAPD Technology to Predict the Herterosis of Zea Mays Sinesis[J];Journal of Beijing Agricultural College;2001-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved