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《Journal of Safety and Environment》 2005-03
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On Zn~(2+) transfer across cell membrane by using bilayer lipid membranes supported by salt bridge

ZHU Xian, SUN Dang-ru, ZHOU Jian-cheng(School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200072, China)  
This paper aims to introduce the authors' simulation of the cell on the bilayer lipid (phosphatidylcholine or cephalin) membranes (BLM) inlaid with cholesterol supported by salt bridge. To this end, we have prepared a great variety of such membranes (whose stability is to exceed 100 hours) by using the octane as solvent, a different ratio of phosphatidyl choline or cephalin to the cholesterol as solute. Our experimental results show that pure lipid can not transfer heavy metal ions while the cholesterol can. The experiments also show that the adsorption really exists between cholesterol and heavy metal ions, and not chemical reaction. The paper has also made a study of the stability of bilayer lipid membranes, the membrane permeation of ions, and the effects of cholesterol on the membrane permeation of ions by means of electrochemical analytical devices. Thus, we have established an adsorption-diffusion simulation model for Zn~(2+) transfer across BLM and calculation of its transfer in conformity with the experimental results.
【Fund】: 国家自然科学基金资助项目(20176026)
【CateGory Index】: Q25
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