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《Journal of Safety and Environment》 2006-06
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Effects of physical and chemical factors on the hemolysis of the Anabaena variabilis hemolysin

SONG Xiu-kai~1,WANG Wei~2,LIU Yun-zhang~2,ZHAO Yuan-yuan~2,RU Shao-guo~2(1 Marine Fisheries Research Institute of Shandong,Yantai 264006,Shandong,China;2 Department of Marine Biology,Ocean University of China,Qingdao 266003,Shandong,China)  
The present paper aims at reporting the authors' research on the ways to determine the hemolytic activities of 20-fold concentrated cell-free supernatants of Anabaena variabilis OL S1 and the effects of some physical and chemical factors on the hemolytic activities by taking the hemolysis of rabbit erythrocytes as an assay.The results show that a hemolytic activity may come about in the cultured medium at the mid-exponential growth phase,gradually intensifying itself with the cell growth,and,then,getting to its maximum size at the stationary phase,which suggests that the hemolytic toxin has been secreted actively into the medium by A.variabilis cells,rather than simply leaking from the ruptured dead cells.The above depicted process also implies that the hemolytic activity of A.variabilis OL S1 is affected significantly by temperature,pH and various metal ions.For example,lower temperature(4 ℃) would almost make the hemolytic activity totally inhibited during the entire 300 min reaction period.Though the hemolytic activities can be enhanced quickly with the increase of temperature from 16 ℃ to 37 ℃ during the first 30 min,they may reach a similar value in 60 min.Moreover,the activity would be intensified in the acid condition(pH 5.0-6.0),and then presents itself little difference in pH 7.2-9.0,thus implying that higher concentration of H~+ helps to facilitate the hemolytic activities.In addition,supplementary metal ions of 4.76(mmol·L~(-1)) Mg~(2+),Ca~(2+) also helps to increase the hemolytic activity,though 4.76(mmol·L~(-1)) Cu~(2+),Mn~(2+) may decrease it significantly.However,4.76 mmol·L~(-1) Ge~(2+),Fe~(3+),Zn~(2+) suggest little effect on it.And,finally,adding 7.62 mg·L~(-1) cholesterol,sphingomyelin and lecithin may distinctively inhibit the hemolysis of A.variabilis hemolytic toxin,thus indicating it is possible to make it combined competitively with the hemolysin, acting as potential receptors of the hemolysin on the erythrocyte membrane.
【Fund】: 国家自然科学基金资助项目(30300034)
【CateGory Index】: X173
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