Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Safety and Environment》 2006-06
Add to Favorite Get Latest Update

Effects of physical and chemical factors on the hemolysis of the Anabaena variabilis hemolysin

SONG Xiu-kai~1,WANG Wei~2,LIU Yun-zhang~2,ZHAO Yuan-yuan~2,RU Shao-guo~2(1 Marine Fisheries Research Institute of Shandong,Yantai 264006,Shandong,China;2 Department of Marine Biology,Ocean University of China,Qingdao 266003,Shandong,China)  
The present paper aims at reporting the authors' research on the ways to determine the hemolytic activities of 20-fold concentrated cell-free supernatants of Anabaena variabilis OL S1 and the effects of some physical and chemical factors on the hemolytic activities by taking the hemolysis of rabbit erythrocytes as an assay.The results show that a hemolytic activity may come about in the cultured medium at the mid-exponential growth phase,gradually intensifying itself with the cell growth,and,then,getting to its maximum size at the stationary phase,which suggests that the hemolytic toxin has been secreted actively into the medium by A.variabilis cells,rather than simply leaking from the ruptured dead cells.The above depicted process also implies that the hemolytic activity of A.variabilis OL S1 is affected significantly by temperature,pH and various metal ions.For example,lower temperature(4 ℃) would almost make the hemolytic activity totally inhibited during the entire 300 min reaction period.Though the hemolytic activities can be enhanced quickly with the increase of temperature from 16 ℃ to 37 ℃ during the first 30 min,they may reach a similar value in 60 min.Moreover,the activity would be intensified in the acid condition(pH 5.0-6.0),and then presents itself little difference in pH 7.2-9.0,thus implying that higher concentration of H~+ helps to facilitate the hemolytic activities.In addition,supplementary metal ions of 4.76(mmol·L~(-1)) Mg~(2+),Ca~(2+) also helps to increase the hemolytic activity,though 4.76(mmol·L~(-1)) Cu~(2+),Mn~(2+) may decrease it significantly.However,4.76 mmol·L~(-1) Ge~(2+),Fe~(3+),Zn~(2+) suggest little effect on it.And,finally,adding 7.62 mg·L~(-1) cholesterol,sphingomyelin and lecithin may distinctively inhibit the hemolysis of A.variabilis hemolytic toxin,thus indicating it is possible to make it combined competitively with the hemolysin, acting as potential receptors of the hemolysin on the erythrocyte membrane.
【Fund】: 国家自然科学基金资助项目(30300034)
【CateGory Index】: X173
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Feng(School of Life Science and Technology,Dalian Fisheries Univ.,Dalian 116023,China);Progresses in research on defence mechanism of echinoderms[J];Journal of Dalian Fisheries University;2005-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 QIN Wen-di et al (Department of Applied Biology, Lishui University, Lishui, Zhejiang 323000);Toxicity Effect of Six Carbamic Ester Pesticides on Anabaena Flos-aquae[J];Journal of Anhui Agricultural Sciences;2005-03
2 LIANG Wen-yu (College of Life Sciences,Ningxia University,Yinchuan,Ningxia 750021);Extraction of Phycocyanin from Nostoc commune Vanch.[J];Journal of Anhui Agricultural Sciences;2008-01
3 CHEN JIEKANg DING GENGWU (Research Institute of Marine fisheries, Liaoning Province);ON THE NEMATOCYSTS OF THE VARIOUS STAGES OF DEVELOPMENT OF RHOPILEMA ESCULENT A KISHINOUYE (CNIDARIA, SCYPHOZOA)[J];Acta Zoologica Sinica;1981-04
4 HUAJinbiao ZONGZhixiang(Department of Biology,Peking University,Beijing,100871);Experimental Research on Formation of Algae Bloom in Yanghe Reservoir[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1994-04
5 LI Xing,ZHAO Liang,YANG Yan-ling(Beijing Key Laboratory of Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China);A Study on the Inactivation of Microcystis Aeruginosa by Manganese-Copper Composite Algaecide[J];Transactions of Beijing Institute of Technology;2009-10
6 NIE Fei1,XIAO Liang1,ZHANG Jing2,HE Qian3,FAN Jun-wen1,LI Yue1,ZHANG Li-ming1 1.Department of Chemical Defense Medicine,Faculty of Naval Medicine,Second Military Medical University,Shanghai 200433,China 2.Department of Pharmacology,School of Pharmacy,Second Military Medical University,Shanghai 200433 3.Department of Gynecology and Obstetrics,Changhai Hospital,Second Military Medical University,Shanghai 200433;Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors[J];Academic Journal of Second Military Medical University;2008-01
7 WANG Cui-hua1,2,LI Guo-feng1,LI Jie1,WU Yan11.Institute of Electrostatic and Special Power,Dalian University of Technology,Dalian 116024,China;2.East China Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Shanghai 200090,China;Study of inactivation effect of Microcystis aeruginosa by pulse discharge[J];Journal of Dalian University of Technology;2009-01
8 HUANG Tinglin,CHAI Beibei(School of Environmental and Municipal Engineering,Xi′an University of Architecture and Technology,State Key Laboratory of Architecture and Technology in Western of China,Xi′an 710055,China);Advances in the Study of Controlling Mechanics and Technology for Source Water Reservoir Quality[J];Advances in Earth Science;2009-06
9 ZENG Xiao\|ping 1,LIU Jann yeng 2,LIN Yun\|fang 1,WANG Ji\|yi 3,XU Chun\|rong 4 (1 Institute of Geophysics, CSB, Beijing 100081, China;2 Institute of Space Science,National Central University,Chungli 320,Taiwan,China;3 Seismological Bureau o;THE POSSIBLE CONNECTION OF THE MANTLE PLUME PHENOMENON WITH THE SEISMO-HYDROMAGNETIC EFFECT PRIOR TO EARTHQUAKES ——Seismo-hydromagnetic effect and a planar current model[J];Earth Science Frontiers;2001-02
10 XU Xingang,ZHANG Yaoxiang,ZHANG qi,ZHANG Qiangzhe,XING Fushan,LI Jianqiang(College of Animal Science and Technology,NorthWest Scietech University of AgriForistry,Yangling,Shaanxi,712100,China);Research of Actinobacillus suis Haemolytic Exotoxin[J];Progress In Veterinary Medicine;2003-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 JIANG Tao a,TENG De-qiang a,JIANG Tian-jiu a,L Song-hui a,YANG Wei-dong b(a.Research Center for Harmful Algae and Aquatic Environment;b.Department of Biology,Jinan University,Guangzhou 510632,China);Advances in Hemolytic Toxins of Marine Microalgae[J];Journal of Tropical and Subtropical Botany;2012-03
2 DONG Ting-ting1,ZHANG Bao-shan1,,SUN Juan1,ZHENG Zhan-wei1,YAO Xiao-wei2 (1.College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710062,China;2.School of Electronical & Machanical Engineering,Xidian University,Xi'an 710071,China);Research progress in edible single cell protein[J];Science and Technology of Food Industry;2012-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved