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《Journal of Safety and Environment》 2010-01
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2-DE analysis in liver of Boleophthalmus pectinirostris during acclimation to cadmium exposure

WU Hai-qing,LI Ming-yun,YE Shuai-dong,CHEN Jiong, SHI Yu-hong (Key Laboratory of Applied Marine Biotechnology,Ministry of Education, Faculty of Life Science and Biotechnology,Ningbo University, Ningbo 315211,Zhejiang,China)  
The aim of the present study is to characterize differental protein exposure profiles in the liver of Boleophlhalmus pectinirostris after cadmium (Cd) exposure through the proteomic approaches.For this purpose, we have developed a two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique for examining the response of the proteome from the liver tissue of Boleophthalmus pectinirostris to test its extremely severe cadmium toxicity.Actually, about 530 protein spots have been detected from the liver samples to a 2D-PAGE gel in the pH range 5-8.Fish of this sort exposed were supposed to be separated into three groups: an acute exposure group (5 mg/L for 3 days), a chronical exposure (0.05 mg/L for 30 days), and a control group (no Cd).The results of our examination show that the total amount of proteins found in the control group, the acute exposure group and chronical exposure group were 512±35, 509±29 and 532 ±22, respectively.The matching rate in average was 90%.For more specific purposes, 14 protein spots were found differentially ex- posed after the acute Cd exposure by two-dimensional gel elec- trophoresis (2-DE).The exposure of the 8 proteins among them were significantly down-regulated, with four of them being up-regulated, one merely seen in the control, and the one added.Seven of them were thern analyzed by using MALDI-TOF-MS.The results of our ex- amination demonstrates that the proteins were K18, prohibitin, GTP- binding protein Ypt1, two-componential system response regulator, similar to T-complex protein 1 subunil theta (TCP-1-theta) ( CCT-theta) , transcriptional regulator, CopG family and mitogen-activated protein kinase homologue, respectively.The fifteen protein spots were found differentially exposed after the chronic Cd.exposure, whereas eight spots among them were found significantly up-regulated, and five down-regulated, with one only noticed in control, and one added.In addition, 6 of them were analyzed by using MALDI-TOF-MS.The re- sults of our examination suggest that the 6 proteins were GTP-binding protein Ypt1, the two-componential system response regulator, hypo- thetical protein BT-1634, keratin 9, and inscriptional regulator CopG family and mitogen-activated protein kinase homologue, respectively. Based on their variable levels and trends on the 2D-PAGE gel, this protein exposure examination suggestes that the finding can be utilized as biomarkers to investigate cadmium pollution degrees in the halobios survival, and evaluate the degree of risk of the human fatalities.In addition, the result of this research reveal that the element cadmium can not only result in differences in protein exposure profiles of Boleophthalmus pectinirostris , but also could induce the homeostasis, even further leading to the apoptosis.
【Fund】: 长江学者和创新团队发展计划项目(IRT0734);; 宁波市重大项目(2008C10022)
【CateGory Index】: X174
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5 WAN Li 1,WANG Nian-bin2,DING Qian3,ZHANG Qi-yue1,YU Bao-chu1(Civil Engineering College,Dalian Fisheries University,Dalian 116023,China;2.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Open Lab of Applied Marine Biotechnology,Liaoning Ocean and Fishery Environmental Monitoring Station,Dalian 116023,China;3.College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China);The Distribution of Heavy Metal Pollution in Jinzhou Bay,Bohai Sea[J];Fisheries Science;2009-12
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8 ZHAO Yan-Feng,YE Neng-Sheng,ZHANG Rong-Li,FENG Xue,LUO Guo-An~*,WANG Yi-Ming(Analysis Center,Department of Chemistry,Tsinghua University,Beijing 100084,China);Different Protein Expression of Myocardium from Chinese Mini-swine Model of Myocardial Infarct[J];Chemical Journal of Chinese Universities;2006-08
9 FANG Cai-Wang1,HUANG Qing-Yu1,2,LING Xue-Ping1,3,KE Cai-Huan2,HUANG He-Qing1,2,3 (1.Key Laboratory of Cell Biology and Tumor Cell Engineering,Ministry of Education,School of Life Sciences,2.State Key Laboratory of Marine Environmental Science,3.Department of Chemical Biology,College of Chemistry & Chemical Engineering,Xiamen University,Xiamen 361005,China);Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt[J];Chemical Journal of Chinese Universities;2010-03
10 CEN Lu,CHEN Lei,ZHANG Xiao-li,ZHANG Shi-hui,LIU Jian-qiang,LU He-feng(Guangzhou Ever-rich Animal Health Co.Ltd,Guangzhou 510830,China);Study on Improvement Effect of Probiotics and Astragalus Polysaccharide in Growth Performance of Piglets[J];China Animal Husbandry & Veterinary Medicine;2009-12
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Peng 1 , LI Cheng-Hua 1 , LI Ye 1 , SU Xiu-Rong 1 , JIN Chun-Hua 1 , LI Tai-Wu 2 (1. School of Marine Sciences, Ningbo University, Ningbo, 315211; 2. Ningbo City College of Vocational Technology, Ningbo, 315100);ISOLATION AND CHARACTERIZATION OF DIFFERENTIAL EXPRESSED PROTEINS FROM SKIN ULCERATION SYNDROME APOSTICHOPUS JAPONICUS[J];Oceanologia et Limnologia Sinica;2013-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Lan YANG Xiu-Qing WANG Qian WANG Ding-Xing (Life Science,Shanxi University,Taiyuan 030006,China);THE ACCUMULATION OF Cd~(2+) AND THE EFFECT ON EST IN FIVE TISSUES AND ORGANS OF ERIOCHEIR SINENSIS[J];Acta Zoologica Sinica;2001-S1
2 LI Rong, LIU Xiao-li, DU Qing-feng, TIAN Hong, SONG Lan-lin, ZHANG Yan, YANG Yi, ZHOU Shu-yun Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510015, China;Experimental study of K562 cell apoptosis induced by harringtonine[J];Journal of First Military Medical University;2002-09
3 DIAO Shu-yong~(1),ZHANG Li-zhi~(2) ,YUAN Hui~(1) (1.College of Animal Science & Technology, Hunan Agricultural University, Changsha,Hunan,410128,China; 2.Iinstutute of Animal Husbandry & Veterinary ,Jinzhou Medicine College,Jinzhou,Liaoning,121001,China);Progress in the Toxicity Mechanism of Cadmium[J];Progress In Veterinary Medicine;2005-05
4 LIU Qian qi CHEN Rong hua QIN Rui ZHANG Jian ning (Department of Pediatrics,Nanjing Medical University,Nanjing 210029,China.);Ultrastructure of splenic immune cell in rats with long term low level lead exposure[J];Journal of Chinese Electron Microscopy Society;2000-06
5 YU Wen bin 1,LI Gu 2,WANG Xue hui 3 (1.Department of Animal Science,Hubei Agricultural College,Jingzhou,Hubei 434103,China; 2.Yangtze River Fisheries Institute,Chinese Academy of Fishery Science,Jingzhou,Hubei 434000, China;3.Hechuan Fisheries Sch;The effect on Micronucleus Rate of Peripheral Blood Erythrocyte of a Few Varieties of Freshwater Fish Induced by Cadmium[J];JOURNAL OF HUBEI AGRICULTURAL COLLEGE;2000-04
6 YE Li qun (Guangzhou habour bureau environmental monitoring station,Guangzhou 510700);Discussion on the Flux of Heavy Meatals into the sea from the Pearl River[J];Environment and Exploitation;2001-02
7 ZHAO Wei Hong, HONG Wan Shu, ZHANG Qi Yong, MA Xi Lan (Department of Oceanography, Xiamen University, Xiamen 361005);STUDIES ON 17α-P AND PGs LEVELS DURING MATURATION AND SPAWNING IN FISHES BOSTRICHTHYS SINENSIS AND BOLEOPHTHALMUS PECTINIROSTRIS[J];Oceanologia Et Limnologia Sinica;2004-01
8 SONG Lin Sheng, JI Yan Bin  , CAI Zhong Hua, FANG Min, SU Jian Guo, CUI Zhao Xia, LI Tai Wu  (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071)  (Life Science and Technology School, Ningbo University, Ningbo, 315211);THE IMMUNOCHEMICAL VARIATION OF MITTEN HAND CRAB ERIOCHEIR SINENSIS AFTER THE INCREMENT OF TEMPERATURE[J];Oceanologia Et Limnologia Sinica;2004-01
9 ZHANG Chun-Dan, HUANG Fu-Yong, LI Ming-Yun, LU Sun-Jie, ZHU Jun-Quan, WU Yong ( Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211);CADMIUM-INDUCED CHANGES IN THE MARKERS OF ERYTHROCYTE MINCRONULEI AND PEROXYDASES IN LIVER OF BOLEOPHTHALMUS PECTINIROSTRIS[J];Oceanologia et Limnologia Sinica;2006-01
10 WEI Xue-tao,SONG Xi ao-ming,XUE Bin(Department of Toxicology,Health Science Center of Peking University,Beijing100083,China);Effect of Cadmium Chloride on the Immunocyte Proliferation and Cell Apoptosis[J];Journal of Labour Medicine;2003-06
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