Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Safety and Environment》 2010-01
Add to Favorite Get Latest Update

2-DE analysis in liver of Boleophthalmus pectinirostris during acclimation to cadmium exposure

WU Hai-qing,LI Ming-yun,YE Shuai-dong,CHEN Jiong, SHI Yu-hong (Key Laboratory of Applied Marine Biotechnology,Ministry of Education, Faculty of Life Science and Biotechnology,Ningbo University, Ningbo 315211,Zhejiang,China)  
The aim of the present study is to characterize differental protein exposure profiles in the liver of Boleophlhalmus pectinirostris after cadmium (Cd) exposure through the proteomic approaches.For this purpose, we have developed a two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique for examining the response of the proteome from the liver tissue of Boleophthalmus pectinirostris to test its extremely severe cadmium toxicity.Actually, about 530 protein spots have been detected from the liver samples to a 2D-PAGE gel in the pH range 5-8.Fish of this sort exposed were supposed to be separated into three groups: an acute exposure group (5 mg/L for 3 days), a chronical exposure (0.05 mg/L for 30 days), and a control group (no Cd).The results of our examination show that the total amount of proteins found in the control group, the acute exposure group and chronical exposure group were 512±35, 509±29 and 532 ±22, respectively.The matching rate in average was 90%.For more specific purposes, 14 protein spots were found differentially ex- posed after the acute Cd exposure by two-dimensional gel elec- trophoresis (2-DE).The exposure of the 8 proteins among them were significantly down-regulated, with four of them being up-regulated, one merely seen in the control, and the one added.Seven of them were thern analyzed by using MALDI-TOF-MS.The results of our ex- amination demonstrates that the proteins were K18, prohibitin, GTP- binding protein Ypt1, two-componential system response regulator, similar to T-complex protein 1 subunil theta (TCP-1-theta) ( CCT-theta) , transcriptional regulator, CopG family and mitogen-activated protein kinase homologue, respectively.The fifteen protein spots were found differentially exposed after the chronic Cd.exposure, whereas eight spots among them were found significantly up-regulated, and five down-regulated, with one only noticed in control, and one added.In addition, 6 of them were analyzed by using MALDI-TOF-MS.The re- sults of our examination suggest that the 6 proteins were GTP-binding protein Ypt1, the two-componential system response regulator, hypo- thetical protein BT-1634, keratin 9, and inscriptional regulator CopG family and mitogen-activated protein kinase homologue, respectively. Based on their variable levels and trends on the 2D-PAGE gel, this protein exposure examination suggestes that the finding can be utilized as biomarkers to investigate cadmium pollution degrees in the halobios survival, and evaluate the degree of risk of the human fatalities.In addition, the result of this research reveal that the element cadmium can not only result in differences in protein exposure profiles of Boleophthalmus pectinirostris , but also could induce the homeostasis, even further leading to the apoptosis.
【Fund】: 长江学者和创新团队发展计划项目(IRT0734);; 宁波市重大项目(2008C10022)
【CateGory Index】: X174
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 Mo Haibo Tian Yi Zhan Yaoyao Zhao Lina Liu Haiying(Key Laboratory of Mariculture,Ministry of Agriculture,Dalian Ocean University,Dalian 116023);Preliminary Establishment of Two-dimensional Gel Electrophoresis for Gonad Tissue of Oratosquilla oratoria[J];Biotechnology Bulletin;2012-04
【Citations】
Chinese Journal Full-text Database 9 Hits
1 CHEN Dan-Ying GAO Yun-Fei LI Li-Xia ZHAI Zhong-He (Life Science College, Peking University, Beijing 100871, China);CHANGES IN KERATIN DURING APOPTOSIS OF PK15 (PORCINE KIDNEY-15) CELLS[J];Acta Zoologica Sinica;2002-01
2 ZHOU Bo 1, YANG Wei 1, ZHANG Jing-Hua 2, TONG Yuan-Peng 1, ZHAO Rui 1, JI Jian-Guo 1+*, RU Bing-Gen 1* (1. Laboratory of Proteome, College of Life Science, Peking University, Beijing 100871, China; 2. Department of Geriatric Neurology, Chinese PLA General Hospital, Beijing 100853, China);Comparative Proteome Analysis of Human Brain Occipital Lobes During the Course of Aging[J];Chemical Research In Chinese Universities;2003-12
3 Meng Xiaohong(205 Teaching and Research Section,Second Artillery Engineering Institute, Xian 710025 );The Biological Accumulation of Cd in Carp[J];TRACE ELEMENTS SCIENCE;1997-01
4 ZHANG Chun-Dan, HUANG Fu-Yong, LI Ming-Yun, LU Sun-Jie, ZHU Jun-Quan, WU Yong ( Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211);CADMIUM-INDUCED CHANGES IN THE MARKERS OF ERYTHROCYTE MINCRONULEI AND PEROXYDASES IN LIVER OF BOLEOPHTHALMUS PECTINIROSTRIS[J];Oceanologia et Limnologia Sinica;2006-01
5 ZHU Ai-Yi, XIE Jia-Yan (Marine Science and Technology School, Zhejiang Ocean University, Zhoushan, 316004);EFFECTS OF CADMIUM ON SERUM TRANSFERRIN AND ERYTHROCYTES OF BOLEOPHTHALMUS PECTINIROSTRIS[J];Oceanologia Et Limnologia Sinica;2007-06
6 LI Ming-yun 1 HUANG Fu-yong 1 ZHU Jun-quan 1 QIAN Li-jun 1 ZHANG Hai-qi 2 ( 1 Faculty of Life Science and Biotechnology, Ningbo University, 315211) ( 2 Zhejiang Fishery Technical Extension Center, Hangzhou 310012);The effects of cadmium ion on the expression of four varieties of isozymes in spleen of Boleophthalmus pectinirostris[J];Marine Fisheries Research;2005-02
7 MU Jin-Ye, CHEN Xiao-Guang(Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China);The progress of the study on the mitogen-activated protein kinase pathways[J];Chinese Bulletin of Life Sciences;2002-04
8 He Guangkai(National Marine Environmental Monitoring Center, Dalian 116023);Levels of heavy metals in molluscs from the coastal region of Bohai Sea and Huanghai Sea[J];CHINA ENVIRONMENTAL SCIENCE;1996-02
9 LI Rong, LIU Xiao-li, DU Qing-feng,ZHANG Song, LUO Rong-cheng, ZHOU Shu-yun. Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China;Proteome analysis of apoptotic K562 cells induced by harringtonine[J];Chinese Journal of Hematology;2004-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiao-hong,ZHANG Hu-fang,WANG Qing(Department of Biology,Taiyuan Teachers College,Taiyuan 030031,Shanxi,China);Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum[J];Carcinogenesis,Teratogenesis & Mutagenesis;2010-05
2 ZHANG Xiao-hong et al (Taiyuan Teachers College,Taiyuan,Shanxi 030031);Effect of Herbicide on EST Isozyme Expression in Goldfish Serum[J];Journal of Anhui Agricultural Sciences;2010-08
3 ZHAO Yue-ping et al (Department of Biology,Taiyuan Teachers College,Taiyua,Shanxi 030031);The Effects of Laundry Powder on POD and EST Isozyme in Blood of Misgurnus anguillicaudatus[J];Journal of Anhui Agricultural Sciences;2010-13
4 ZHANG Cui1,2,ZHAI Yu-xiu1,2,NING Jin-song2,3,SHANG De-rong2,3,WANG Jia-lin3(1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.National Center for Quality Supervision and Test of Fishery Products,Qingdao 266071,China;3.Ocean University of China,Qingdao 266003,China);A Review: Research on Cadmium in Aquatic Animals[J];Fisheries Science;2007-08
5 WAN Li 1,WANG Nian-bin2,DING Qian3,ZHANG Qi-yue1,YU Bao-chu1(Civil Engineering College,Dalian Fisheries University,Dalian 116023,China;2.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Open Lab of Applied Marine Biotechnology,Liaoning Ocean and Fishery Environmental Monitoring Station,Dalian 116023,China;3.College of Environmental Science and Engineering,Dalian Maritime University,Dalian 116026,China);The Distribution of Heavy Metal Pollution in Jinzhou Bay,Bohai Sea[J];Fisheries Science;2009-12
6 LIU Tian-hong,SUN Fu-xin,WANG Ying,WU Zhi-hong,SUN Yuan-qin,LI Xiao,LU Jun(Shandong Provincial Mariculture Institute,Qingdao 266002,China);The Acute Toxicity of Copper Sulfate to Scallop Chlamys farreri[J];Fisheries Science;2011-06
7 PANG Yan-hua,JIANG Wei,XUE Da-fang,HUANG Da-liang(Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116001,China);Heavy Metal Contents and Quality Evaluation in Japanese Scallop Patinopecten yessoensis in Coastal Dalian[J];Fisheries Science;2012-03
8 YANG Ping, JIA Yu-hua, LI Jie, ZHOU Feng-hua, LI Li-jun Southern Medical University, Guangzhou 510515, China;Anti-oxidation of Tanshinone Ⅱ_A and Prohibitin on Cardiomyocytes[J];中草药(英文版);2010-03
9 ZHAO Yan-lin1,PENG Long1,ZHAO Jin1,LIU Meng-jun2(1.College of Life Science,Agricultural University of Hebei,Baoding 071001,China;2.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding 071001,China);Cloning and analysis of MAPK gene fragment from Ziziphus jujuba Mill.[J];Journal of Agricultural University of Hebei;2010-06
10 SUN Wei-ping1,2,PAN Jian-ming1,LIU Xiao-ya1,HUANG Yong-xiang3,ZHENG Min-hui1(1.Second Institute of Oceanography,SOA,Laboratory of Ecosystem and Biogeochemistry,SOA,Hangzhou 310012,China;2.Institute of Environment and Biogeochemistry,Zhejiang University,Hangzhou 310027,China;3.Department of Science and Engineering,Jinhua Telecasting University,Jinhua 321000,China);Study of the content of heavy metals in the mollusks from the near-shore of Zhejiang Province[J];Journal of Marine Sciences;2010-04
China Proceedings of conference Full-text Database 3 Hits
1 Chen Da-rui 1 Shen Zhong-yuan 1,2,3* Tang Xu-dong 1,2,3 Xu Li 1,2,3 Zhu Feng 2 Tao Heng-ping 2 Guan Rui 2 (1 Jiangsu University of Science and echnology,Zhenjiang Jiangsu 212018,China;2 The Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China);The study of the inhibition effects of albendazole on Nosema bombycis β-tubulin gene expression[A];[C];2010
2 Ruan Jinshan1,Zhong Shuoliang1,Lin Houxiang2,Yang Miaofeng1, Zheng Shenghua1,Qian Xiaoming1 (1.Fujian Fishery Research Institute,Xiamen 361013;2.Oceanic and Fishery Bureau of Lianjiang county,Lianjiang,Fujian,350500);Quality security assessment of shellfish-culture Lianjiang southeast sea area,Fujian[A];[C];2010
3 ;The Mitogen-Activated Protein Kinase and Its Signal Transport in Plants[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YU Shui-lan,SONG Gao-chen,YU He,et al(1.Heilongjiang University of Traditional Chinese Medicine,Heilongjiang Harbin 150040;2.Mudanjiang Medical University,Heilongjiang Mudanjiang 157011);Study on the Effects of GAPSGF on the Expression of Soluble Proteome of Mouse HepA Carcinoma by 2-DE Technology[J];Journal of Chengdu University of Traditional Chinese Medicine;2009-01
2 HU Zhong-ze, JIN Guang-ming, WANG Li-ke, YANG Jiu-feng(Department of Animal Science, Anhui Technical Teachers College, Fengyang 233100, China);Effect of Tea Polysaccharides on Immune Functions and Antioxdative Activity in Broilers[J];Journal of Tea Science;2005-01
3 CHEN Qing-hua LIU Zhu-ying HE Jian-hua(College of Animal Science and Technology,Hunan Agriculture University,Changsha 410128,China);Effects of Achyranthes Bidentate Polysaccharide on the Proliferation of Lymphocytes and Concentrations of Cytokine in Peripheral Blood Mononuclear Cells of Piglets[J];Chinese Journal of Animal Nutrition;2008-06
4 LIANG Ying1 JIANG Ning2 HE Wenjuan3 REN Chengcai2 WANG Deli1 ZHENG Wenfeng1 WANG Juntao1(1.College of Arts and Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;3.Xilingol Vocational College,Xilinhaote 026000,China);Effects of Astragalus Polysaccharide on Growth Performance and Immune Function of Broilers[J];Chinese Journal of Animal Nutrition;2010-04
5 ZHANG Meng jie 1, REN Qing guang 2, CHEN Yong qing 1(1 Department of Microbiology,Fudan University, Shanghai 200433,China; 2 Research Center for Analysis & Measurement,Fudan University, Shanghai 200433,China);Studies on the Antitumor Effect of Glycoproteins from Enterobacter Cloacae[J];Journal of Fudan University;2002-04
6 Bao Xiao-Dong1,2,Huang Lin1,2,Huang He-Qing 1,2,3,Cai Zong-Wei4 1(The Key Laboratory of Ministry of Education for Cell Biology and Tumo r Cell Engineering,School of Life Sciences;2The Key Laboratory of Chemical Biology of Fujian Province,State Key Laboratory of Physical Chemistry of Solid Surface Collage;3Chemistry & Chemical Engineering,Xiamen University,Xiamen 361005)4(Department of Chemistry,Hong Kong Baptist University,Hong Kong);Selection and Identification of Acute Proteins with Proteomic Techniques in Rhinencephalon of Cyprinus Carpio after Wounded Acutely[J];Chinese Journal of Analytical Chemistry;2008-04
7 ZHOU Bo 1, YANG Wei 1, ZHANG Jing-Hua 2, TONG Yuan-Peng 1, ZHAO Rui 1, JI Jian-Guo 1+*, RU Bing-Gen 1* (1. Laboratory of Proteome, College of Life Science, Peking University, Beijing 100871, China; 2. Department of Geriatric Neurology, Chinese PLA General Hospital, Beijing 100853, China);Comparative Proteome Analysis of Human Brain Occipital Lobes During the Course of Aging[J];Chemical Research In Chinese Universities;2003-12
8 ZHAO Yan-Feng,YE Neng-Sheng,ZHANG Rong-Li,FENG Xue,LUO Guo-An~*,WANG Yi-Ming(Analysis Center,Department of Chemistry,Tsinghua University,Beijing 100084,China);Different Protein Expression of Myocardium from Chinese Mini-swine Model of Myocardial Infarct[J];Chemical Journal of Chinese Universities;2006-08
9 FANG Cai-Wang1,HUANG Qing-Yu1,2,LING Xue-Ping1,3,KE Cai-Huan2,HUANG He-Qing1,2,3 (1.Key Laboratory of Cell Biology and Tumor Cell Engineering,Ministry of Education,School of Life Sciences,2.State Key Laboratory of Marine Environmental Science,3.Department of Chemical Biology,College of Chemistry & Chemical Engineering,Xiamen University,Xiamen 361005,China);Stress Proteins of Gill Tissue in Patinopecten yessoensis Exposed to Cadmium Salt[J];Chemical Journal of Chinese Universities;2010-03
10 CEN Lu,CHEN Lei,ZHANG Xiao-li,ZHANG Shi-hui,LIU Jian-qiang,LU He-feng(Guangzhou Ever-rich Animal Health Co.Ltd,Guangzhou 510830,China);Study on Improvement Effect of Probiotics and Astragalus Polysaccharide in Growth Performance of Piglets[J];China Animal Husbandry & Veterinary Medicine;2009-12
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 ZHANG Peng 1 , LI Cheng-Hua 1 , LI Ye 1 , SU Xiu-Rong 1 , JIN Chun-Hua 1 , LI Tai-Wu 2 (1. School of Marine Sciences, Ningbo University, Ningbo, 315211; 2. Ningbo City College of Vocational Technology, Ningbo, 315100);ISOLATION AND CHARACTERIZATION OF DIFFERENTIAL EXPRESSED PROTEINS FROM SKIN ULCERATION SYNDROME APOSTICHOPUS JAPONICUS[J];Oceanologia et Limnologia Sinica;2013-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Lan YANG Xiu-Qing WANG Qian WANG Ding-Xing (Life Science,Shanxi University,Taiyuan 030006,China);THE ACCUMULATION OF Cd~(2+) AND THE EFFECT ON EST IN FIVE TISSUES AND ORGANS OF ERIOCHEIR SINENSIS[J];Acta Zoologica Sinica;2001-S1
2 LI Rong, LIU Xiao-li, DU Qing-feng, TIAN Hong, SONG Lan-lin, ZHANG Yan, YANG Yi, ZHOU Shu-yun Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510015, China;Experimental study of K562 cell apoptosis induced by harringtonine[J];Journal of First Military Medical University;2002-09
3 DIAO Shu-yong~(1),ZHANG Li-zhi~(2) ,YUAN Hui~(1) (1.College of Animal Science & Technology, Hunan Agricultural University, Changsha,Hunan,410128,China; 2.Iinstutute of Animal Husbandry & Veterinary ,Jinzhou Medicine College,Jinzhou,Liaoning,121001,China);Progress in the Toxicity Mechanism of Cadmium[J];Progress In Veterinary Medicine;2005-05
4 LIU Qian qi CHEN Rong hua QIN Rui ZHANG Jian ning (Department of Pediatrics,Nanjing Medical University,Nanjing 210029,China.);Ultrastructure of splenic immune cell in rats with long term low level lead exposure[J];Journal of Chinese Electron Microscopy Society;2000-06
5 YU Wen bin 1,LI Gu 2,WANG Xue hui 3 (1.Department of Animal Science,Hubei Agricultural College,Jingzhou,Hubei 434103,China; 2.Yangtze River Fisheries Institute,Chinese Academy of Fishery Science,Jingzhou,Hubei 434000, China;3.Hechuan Fisheries Sch;The effect on Micronucleus Rate of Peripheral Blood Erythrocyte of a Few Varieties of Freshwater Fish Induced by Cadmium[J];JOURNAL OF HUBEI AGRICULTURAL COLLEGE;2000-04
6 YE Li qun (Guangzhou habour bureau environmental monitoring station,Guangzhou 510700);Discussion on the Flux of Heavy Meatals into the sea from the Pearl River[J];Environment and Exploitation;2001-02
7 ZHAO Wei Hong, HONG Wan Shu, ZHANG Qi Yong, MA Xi Lan (Department of Oceanography, Xiamen University, Xiamen 361005);STUDIES ON 17α-P AND PGs LEVELS DURING MATURATION AND SPAWNING IN FISHES BOSTRICHTHYS SINENSIS AND BOLEOPHTHALMUS PECTINIROSTRIS[J];Oceanologia Et Limnologia Sinica;2004-01
8 SONG Lin Sheng, JI Yan Bin  , CAI Zhong Hua, FANG Min, SU Jian Guo, CUI Zhao Xia, LI Tai Wu  (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071)  (Life Science and Technology School, Ningbo University, Ningbo, 315211);THE IMMUNOCHEMICAL VARIATION OF MITTEN HAND CRAB ERIOCHEIR SINENSIS AFTER THE INCREMENT OF TEMPERATURE[J];Oceanologia Et Limnologia Sinica;2004-01
9 ZHANG Chun-Dan, HUANG Fu-Yong, LI Ming-Yun, LU Sun-Jie, ZHU Jun-Quan, WU Yong ( Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211);CADMIUM-INDUCED CHANGES IN THE MARKERS OF ERYTHROCYTE MINCRONULEI AND PEROXYDASES IN LIVER OF BOLEOPHTHALMUS PECTINIROSTRIS[J];Oceanologia et Limnologia Sinica;2006-01
10 WEI Xue-tao,SONG Xi ao-ming,XUE Bin(Department of Toxicology,Health Science Center of Peking University,Beijing100083,China);Effect of Cadmium Chloride on the Immunocyte Proliferation and Cell Apoptosis[J];Journal of Labour Medicine;2003-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved