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《Journal of Safety and Environment》 2012-02
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On the acidic metabolite characterization and degradation process of the phenanthrene by Pseudomonas aeruginosa NY3

SHEN Xiao-juan,NIE Mai-qian,GE Bi-zhou,FAN Yu,WANG Fei,CHANG Hong(School of Environmental and Municipal Engineering,Xi'an University of Architecture and Technology,Xi'an 710055,China)  
This paper deals with a study on the acidic metabolite characterization and degradation process of the phenanthrene by means of Pseudomonas aeruginosa NY3.As is known,though there has been a lot of articles published on the study of metabolites,yet little has been reported on the biodegradation pathways of polycyclic aromatic hydrocarbons by pseudomonas aeruginosa.Here in this paper we would like to report the typical acidic metabolites of phenanthrene and its degrading pathway by P.aeruginosa NY3,which can help to metabolize hydrophobic compounds quickly.P.aeruginosa NY3,as a matter of fact,could grow on the phenanthrene well and its metabolites such as α-naphthol,salicylic acid,benzoic acid in regard with its sole carbon source and energy.GC-MS data indicate that it is possible to find five major metabolites of phenanthrene as the bacteria grow.They are 1-hydroxy-2-naphthoic acid,2-hydroxy-2-carboxylic acid,α-naphthol,o-hydroxyphenyl carbonyl acid,salicylic acid,and benzoic acid.Based on the analysis of metabolites,the biodegradation pathway can be deduced.First of all,the phenanthrene can be made transformed into 1-hydroxy-2-naphthoic acid after a series of oxidative metabolism steps by P.aeruginosa NY3,and later into α-naphthol through decarboxylation.Other metabolites that can be transformed from phenanthrene into 1-hydroxy-2-naphthoic acid can be metabolized so fast that it won't be possible to be found in our experiments,though it would be possible to find one-aromatic-ring metabolites like o-hydroxyphenyl carbonyl acid,salicylic acid,benzoic acid were detected was the evidence of further transformation of α-naphthol.However,it wouldn't be possible to find dihydroxynaphthalene,dihydroxybenzene and other polyphenols could as the metabolites of phenanthrene by pseudomonas aeruginosa NY3,while there were greater amount of metabolites which can be characterized as ortho-hydroxyl carboxyl compounds,such as 1-hydroxy-2-naphthoic acid and salicylic acid which were easily accumulated.This means that the decarboxylation by NY3 is not an easy step and ortho-hydroxyl carboxyl compound rarely transformed into dihenols.α-naphthol and benzoic acid were also somewhat accumulated.And therefore,o-hydroxyphenyl acetic acid has actually been the first report of this nature.
【CateGory Index】: X172
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