Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anqing Teachers College(Natural Science Edition)》 2009-02
Add to Favorite Get Latest Update

Studying on the Interaction Between Zn~(2+) and Polyphenol Oxidase from Apple

XIAO Hou-rong,WANG Zhong-feng,YANG Hong,WU Qian-qian,ZHANG Jie,CAI Jing-min (Department of Biological and Environmental Engineering,Hefei University,Hefei 230022,China)  
The effect of exo—Zn~(2+) on the structure and activity of Polyphenol Oxidase(PPO) is studied by employing enzymatic activity assay,differential absorption spectrum,synchronous fluorescence spectra and far—UV CD spectra, which could be used to explore the relations between their structure and function.The results show that the activity of PPO is enhanced by trial Zn~(2+),reaching its maxium at[Zn~(2+)]/[PPO]2.0,but inhibited when Zn~(2+) is further added. Trial Zn~(2+) can also increase and theα—helix content.Zn~(2+) may coordinate with histidine(s) in PPO,and the absorptions of(ImH)σ_N+σ_N→Zn~(2+),(ImH)σ→Zn~(2+),(ImH)π_2→Zn~(2+),(ImH)π_1→Zn~(2+) occur at 227,241,265,335nm,respectively. Synchronous fluorescence spectra exhibit that microenvironment of Trp residue in PPO is more hydrophobic than that of free Trp in water,and that both fluorescences of Trp and Tyr are quenched by Zn~(2+),but the microenvironments of both Trp and Tyr residues in PPO undergo no changes with Zn~(2+) addition.
【Fund】: 合肥学院引进人才资金(600805)资助
【CateGory Index】: S661.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 GONG Wu-Xia,LI Shu-Yan,JIANG Jun,CHEN Fang, ZHANG Yan,HU Xiao-Song,WU Ji-Hong(College of Food Science and Nutritional Engineering,China Agricultural University; Key Laboratory of Fruits and Vegetables Processing,Ministry of Agriculture; Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education,Beijing 100083,China);Effect of Pressure Combined with Metal Ions on Polyphenol Oxidase (PPO) Activity from Raspberry[J];Chinese Journal of High Pressure Physics;2012-05
【Citations】
Chinese Journal Full-text Database 9 Hits
1 Chou Ju,Du Jiangyan,Feng Yuying,Yang Xiujuan,Lu Tianhong (Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022) (College of Chemistry and Enironment Science,Nanjing Normal University,Nanjing 210097);Synchronous Fluorescence Spectra of Myoglobin[J];Chinese Journal of Analytieal Chemistry;2001-02
2 FENG Xi-Zeng;JIN RuiXiang;QU Yun; HE Xi-Wen;(Department of Chemistry,Nankai University,Tianjin,300071);Studies on the Ions' Effect on the Binding Interaction Between HP and BSA[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-06
3 HE Jin-tian 1,WANG Gai-zhen 2 1 Enterprise Technology Centre,Shijiazhuang Pharm.Group,Shijiazhuang 050041,China; 2 College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China;Study on the LMCT Band of Zinc-bovine Serum Albumin[J];Spectroscopy and Spectral Analysis;2001-05
4 YI Ping gui 1,LIU Jun feng 1,SHANG Zhi cai 2,and YU Qing sen 1 1 Department of Chemical Engineering,Xiangtan Polytechnic University,Xiangtan 411201,China 2 Department of Chemistry,Zhejiang University,Hangzhou 310027,China;Study on the Interaction between Methylene Blue and Bovine Serum Albumin by Fluorescence Spectroscopy[J];Spectroscopy and Spectral Analysis;2001-06
5 ZHANG Chaoping and XIA Min(Department of Chemistry,Guizhou University, 550025 Guiyang);THE FORMATION AND SPECTRA OF THE PROTEINS-SILVERCOPPER COMPLEXES[J];SPECTROSCOPY AND SPECTRAL ANALYSIS;1996-05
6 Tianzhi YU Department of Chemistry,Northwest Normal University,730070 Lanzhou Zuyi TAO Department of Modern Physics,Lanzhou University,730000 Lanzhou;Fluorescence Study on the Interaction of Salicylic Acid and Human Serum Albumin[J];SPECTROSCOPY AND SPECTRAL ANALYSIS;1999-03
7 [KH*2D〗Liang Xian Zhen (Shanghai Institute of Materia Medica,Chinese Academy of Science,Shanghai 200032) Li Lian Zhi Hu Xu Ying Zhou Yong Qia Shen Pan Wen (The Department of Chemistry,Nankai University,Tianjin 300071);The Study on the UV Spectra of the Active Center of Superoxide Dismutase of Bovine Erythrocytes[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1998-02
8 XIAO Hou-Rong SHI Chun-Hua XIA Bing-Le SHENG Liang-Quan ZHANG Yan-Ge LIU Qing-Liang? ?Department of Chemistry, University of Science and Technology of China, Hefei 230026);Study on the Interaction between Cu~(2+)and Polyphenol Oxidase from Nicotinna Tobaccum[J];Chinese Journal of Inorganic Chemistry;2003-06
9 Li Xiaojing; Wang Zhiqiang; Chen Ji; Zhang Shugong(Laboratory of Rare Earth Chemistry and Physics,Changchun Institute ofApplied Chemistry,Chinese Academy of Sciences,Changchun 130022);UV Spectral Study on the Interaction of RE Ions with BSA[J];CHINESE JOURNAL OF APPLIED CHEMISTRY;1998-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU De-chuan et al (Agriculture College,Anhui Agricultural University,Hefei,Anhui 230026);Researeh Advances in Polyphenol Oxidase in Tobacco Plants and the Relations between PPO and Tobacco Leaf Quality[J];Journal of Anhui Agricultural Sciences;2006-07
2 LIU Wei et al(Hainan Key Lab.of Tropical Medicinal Herb and Chemistry,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,Hainan 571158);Study on the Interaction between Aspirin and DNA by Spectrometric Method[J];Journal of Anhui Agricultural Sciences;2009-15
3 LIU Wei et al(College of Chemistry and Chemical Engineering,Hainan Normal University,Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,Haikou,Hainan 571158);Research on the Interaction of Resveratrol and DNA[J];Journal of Anhui Agricultural Sciences;2009-28
4 QU Ran ZHANG Xiao ZHANG Peng(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);The interaction between L-citrulline and bovine serum albumin[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2012-01
5 Wang Teng1 He Xiaonan1 Zhang Zhihong1 Guo Xiaoyan1Zhang Lihui1 Zhang Yi1 Xiang Bingren2(1.Department of Pharmaceutical Engineering,Beijing Institute of Petro-chemical Technology,Beijing 102617,China; 2.Center for Instrumental Analysis,China Pharmaceutical University,Nanjing 210009,China);Spectroscopic Studies on the Interaction of Azithromycin and Human Serum Albumin[J];Journal of Beijing Institute of Petro-Chemical Technology;2011-03
6 GAO Wen-jing,YIN Fan(School of Chemistry and Materials Engineering,Changshu Institute of Technology,Changshu 215500,China);A Study of Interaction between Salicylic Acid and Bovine Serum Albumin by Fluorescence Spectrographic Methods[J];Journal of Changshu Institute of Technology;2010-08
7 HAN Tian-tian,LING Jing,DAI Xiao-li,LEI Yan,LI Rui-ping(Engineering Research Center of Eco-environment in Three Gorges Reservoir Region of Ministry of Education,China Three Gorges University,Yichang 443002,China);Interactions between Heavy Metal Ions and Enoxacin by Fluorescence Spectroscopy[J];Environmental Science & Technology;2012-04
8 Liu Yongming *, Li Guizhi, Sun Xifen (College of Chemistry, Yantai University, Yantai 264005);Study on the Interaction between Colchicine and Bovine Serum Albumins by Fluorescence Method[J];Chinese Journal of Analytical Chemistry;2004-05
9 Chang Xijun *, Huang Yan, Wang Sui (Department of Chemistry and Chemical Engineering , Lanzhou University , Lanzhou 730000);Interaction between Pd(Ⅱ) and Gamma Seroglobulinum Humanum[J];Chinese Journal of Analytical Chemistry;2005-01
10 Wang Qun *1, Shi Jing 2, Zhang Huan 2, Ni Jiazuan 3 1(Pharmaceutical College, Shanghai Jiaotong University, Shanghai 200030) 2(Changchun Life Gene Pharmaceutical Limited Company, Changchun 130022) 3(Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022);Study on the Interaction between Recombinant Endostatin with Aloe by Fluorescence and Circular Dichroism Methods[J];Chinese Journal of Analytical Chemistry;2005-07
China Proceedings of conference Full-text Database 3 Hits
1 Hongmin Ma, Jin Wang, Shuyuan Liu, Tao Yan, Wei Yu, Yan Li and *Qin Wei School of Chemistry & Chemical Engineering, Jinan University, 250022, Jinan;Thermodynamic Studies on the Interaction between Lumogallion and Bovine Serum Albumin by Fluorescence Quenching Technique[A];[C];2006
2 Xiao Hourong~1 Zhao Bing~2 Li Qiang~2 Wei Jiaxin~2 Yang Chuanmin~3 (1 Tobacco Research Centre, University of Science and Technology of China, Hefei, 230026) (2 Technology and Research Center, Nanyang Cigarette Factory, Henan, 473007) (2 Technology and Research Center, Chuzhou Cigarette Factory, Anhui, 239000);Studies on the Interaction Mechanism for Cystine and Polyphenol Oxidase from Nicotaina Tobacum[A];[C];2004
3 AODENG Gao-wa,WANG Xiao-xia~* College of Chemistry and Chemical Engineering,Inner Mongolia University,Huhhot 010021,China;Studies on the Interaction between Phenylbutazone,Copper(Ⅱ) and Protein by Fluorometry[A];[C];2010
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CI Zhi-juan,CHEN Xue-sen~*,ZHANG Li-jie,LIANG Qing,ZHANG Chun-yu,LIU Xiao-li(Biological Laboratory of Fruit Trees,Shandong Agricultural University,Taian 271018,China);Preliminary Study on the Mechanism of Enzymatic Browning in Apricot Fruits[J];Shandong Agricultural Sciences;2006-06
2 QIAO Fang et al(Applied Biotechnical Department,Shenzhen Polytechnic,Shenzhen,Guangdong 518055);Research Advance in the Enzymatic Browning and its Inhibition Method of Fruit and Vegetable[J];Journal of Anhui Agricultural Sciences;2007-24
3 ZHAO Yan et al(Department of Life Science,Chifeng College,Chifeng,Inner Mongolia 024000);Activity Determination and Property Analysis on Peroxydase in Onion[J];Journal of Anhui Agricultural Sciences;2011-03
4 ZHANG Jing-zheng,QI Yong-shun,WANG Tong-kun,LI Xiao-li(Dept.of Horticulture and Landscape Architecture,Hebei Normal University of Science & Technology,Changli,066600,China);A New Method for Measuring Browning of Chestnut Using Digital Camera[J];Northern Horticulture;2008-04
5 GAO Hong-yan,XU Qiang,YANG Zhi-huai(Dept.Phys.& Infom.Tech.,Baoji Univ.Arts & Sci.,Baoji 721007,Shaanxi,China);Research on protein secondary structure by Fourier transformation infrared spectroscopy[J];Journal of Baoji University of Arts and Sciences(Natural Science Edition);2009-03
6 WANG Qing-zhang,LI Jie(Food Science and Technology Department,Central China Agricultural University,Wuhan 430070,China);Effect of Package Materials on Keeping Quality of Lotus Root[J];Storage and Process;2002-02
7 YAN Shou -lei,WANG Qing -zhang,PENG Guang -hua (Food Science Department,Huazhong Agricultural University,Wuhan 430073,China);Exploration of Boiled Lotus Root Slices Processing Technology[J];Storage and Process;2002-03
8 CHENG Jian jun,REN Yun hong,YANG Yong li LI Mei,GAO Chan (Northeast Agricultural University Food Science College Harbin Heilongjiang 150030,PRC);Recent progress in inhibition of enzymatic browning of fruit and vegetable[J];Journal of Northeast Agricultural University;2000-04
9 GAO Hui,RAO Jing-ping (College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China);Effect of Chilling Injury on Membrane Fatty Acid and Enzymatic Activity of Stored Nectarines[J];Acta Botanica Boreali-Occidentalia Sinica;2007-04
10 Lü Hai-Tao,Sun Hai-Feng,Qu Bao-Han,Dai Hong-Yi (Qingdao Agricultural University,Qingdao 266109);Simultaneous Determination of Six Phenolic Compounds in Apple Juice by High Performance Liquid Chromatography[J];Chinese Journal of Analytical Chemistry;2007-10
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 JIANG Qin-ren1,MA Ya-mei1,MA Yong-qiang1,XIONG Hui-yan2,DUAN Rui-jun1(1.Department of Biological Sciences,Qinghai University,Xining,Qinghai 810016;2.College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai 810016);Study on Changes of Related Physiological Index of Ruaner Pear at Frozen Storage Process[J];Northern Horticulture;2013-03
2 ZHAO Yun-feng 1,2,LIN Yu 3,WU Ling-yan 1(1.Yancheng Institute of Technology,Yancheng 224051;2.Fujian Agriculture and Forestry University,Fuzhou 350002;3.Department of Resources and Engineering,Minxi Vocational and Technical College,Longyan 364021);Effects of heat treatment on browning and metabolism of phenolics in cold-stored eggplant fruits[J];Food Science and Technology;2012-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Feng Xizeng.He Xiwen (Department of Chemistry,Nankai University,Tianjin 300071);Interaction of Metal Ions-Hematoporphyrin Under Physiological Condition by Ultraviolet Spectrophotometry[J];Chinese Journal of Analytical Chemistry;1993-11
2 Zhou Yong-Qia; ZHANG Xi-Quan; HE Jin-Tian; LIANG Xian-Zhen; OUYANG Di(Department of Chemistry, Nankai University, Tianjin, 300071)HU Tian-Dou, XIE Ya-Ning(Beijing Electron Position Collider National Laboratory, Beijing, 100039);Studies on the Structure of Metal Serum Albumin(Ⅷ)──A Fluorescence EXAFS Study of the Zinc Ion Centers of Zn(Ⅱ)-HSA and Zn(Ⅱ)-BSA Complexes[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-06
3 ZHANG Bao-Lin; WANG Wen-Qing; BAI Feng-Lian(Coordination Chemistry Institute of Nanjing University,State Key Laboratory of Coordination Chemistry, Nanjing, 210008) (Technical Physics Department of Peking University) (Institute of Chemistry,Chinese Academy;Studies on the Binding of Anthraquinones and Flavonoids to Human Serum Albumin[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1994-03
4 HE Xi-WEn; FENG Xi-Zeng; ZHANG Gui-Zhu; YANG Wan-Long;YU Gui-Ying; SHI Hui-Ming(Department of Chemistry, Nankai University, Tianjin, 300071);Studies on the State of Hematoporphyrin(HP) in Physiological Condition Solution[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1994-04
5 FENG Xi-Zeng;JIN RuiXiang;QU Yun; HE Xi-Wen;(Department of Chemistry,Nankai University,Tianjin,300071);Studies on the Ions' Effect on the Binding Interaction Between HP and BSA[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1996-06
6 Yiping TONG,Wei LI and Yanwen LIN Department of Bio Chemistry,Hanshan Teacher′s College,521041 Chaozhou;Study of Conformation of Serum Albumin by FTIR[J];SPECTROSCOPY AND SPECTRAL ANALYSIS;1999-05
7 LU Jin-Xin(Department of Pharmacy, Tianjin Madical University, Tianjin ,300203)ZHANG Gui-Zhu ZHAO Peng HE Xi-Wen SHI Hui- Ming(Department of Chemistry, Nankai University, Tianjin, 300071);Studies on Interaction between Adriamycin and Serum Albumin as well as Effect of Ions on the Reaction[J];Acta Chimica Sinica;1997-09
8 MA Chun - Qi LI Ke - An ZHAO Feng - Lin TONG Shen - Yang (Department of Chemistry, Peking University, Beijing, 100871);A Study on the Reaction Mechanism between Chrome-Azurol S and Bovine Serum Albumin[J];Acta Chimica Sinica;1999-04
9 YI Ping - Gui SHANG Zhi - Cai YU Qing - Sen SHAO Shuang LIN Rui - Sen (Department of Chemistry, Zhejiang University, Hangzhou, 310027) ( Department of Chemical Engineering, Xiangtan Polytechnic University, Xiangtan, 411201);Studies on the Interaction between Mytomycin C and Bovine Serum Albumin[J];Acta Chimica Sinica;2000-12
10 YANG Binsheng (Institute of Molecular Science,Shanxi University,Taiyuan 030006) Wesely R.HARRIS (Department of Chemistry,University of Missouri St Louis,St Louis,MO 63121,USA);Fluorescent Study on the Binding of Terbium Ion with Apotransferrin[J];CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved