ISOLATION AND BIOLOGICAL CHARACTERISTICS OF UREAPLASMA UREALYTICUM
Ye Yuankang, et al( Department of Mkrobiolgy Bengbu Medical College, Anhui )
There are 3 strains of Ureaplasma urealyticum isolated from 5 vaginal specimens, befors or , niter delivery, with modified Taylor-Robinson liquid medium. Ureaplasma urealyticum grows rapidly and could break down urea into alkaline which will change the phenol red in the med ia to red color within 12-24 hours, but the liquid may remain clear. The pass of the culture through 0.45 μm membrane filter may produce the same phenomenon on transferring the filtrate into new medium. On solid media after incubating for 24 hours in a candle jar, the whole agar may become alkaline and turn into red color, tiny round colonies 15-30 μm in diameter can be seen in fine granules under microscope (×100). On prolonged incubation, colonies increase in size to 60-80 μm in diameter, with a typical appearance of fried egg. No cell wall can be found in. an individual organism under electron micrography. Growth conditions, nutritive requirements, biochemical reactions of Ureaplasma urealylicum are also discussed in this article.