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《Journal of Bengbu Medical College》 2003-03
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Comparison of the micro clonogenic,MTT and the clonogenic assays for SF_2 testing in human nasopharyngeal carcinoma cell lines

JIANG Hao,XIA Yun fei,QIAN Jian yang,CUI Nian ji,LIANG Yong ju(Department of Radiotherapy, Cancer Center,Sun yat sen University, Guangzhou 510060,China)[  
Objective:To compare the micro clonogenic,MTT and the clonogenic assays for SF 2 testing in human nasopharyngeal carcinoma cell lines. Methods:The nasopharyngeal carcinoma cell lines CNE1, CNE2, SUNE, SUNE1 were selected and the SF 2 of each cell line was detected three times with the above three assays, respectively. Results:There were obvious positive correlations between the SF 2 values of the four cell lines estimated with the three methods. The linear correlation coefficients were 0.973, 0.994 and 0.973( P 0.01), respectively. Conclusions:The MTT assay and micro clonogenic assay can replace the traditional clonogenic assay for detecting SF 2 of cell lines, and both methods are economical,convenient and reasonable. [
【Fund】: 中山大学 (原中山医科大学 ) 2 11工程资助 (编号 :980 5 9)
【CateGory Index】: R739.63
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