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Detection of reference limits of the reticulocyte counts using CELL-DYN 3700 system

XU Wen long,NI Ke ming,ZHANG Guo xiang,ZHANG Qun wei,ZHANG Jing hua (Department of Clinical Laboratory,Xiaoshan Maternity and Parameter Child Health Hospital,Zhejiang 311200,China)  
Objective:To establish a reference limit of the reticulocyte counts using CELL DYN 3700 system.Methods:The reticulocytes in 102 apparently healthy subjects of both sexes were counted by CELL DYN 3700 system and the data were statistically analysed.The subjects were divided into two groups according to sex.Results:In men aged 21 to 39 yr old,the percentage of reticulocyte in total erythrocytes(RET%) was 1.80± 0.35 ; in women aged 20 to 39 yr old,the RET% was 1.76±0.31.The difference was not obvious( P 0.05).In men,the reticulocyte absolute value RET#(×10\+9/L) was 91.4±28.3;in women,the RET# was 72.3± 25.6 .The difference was obvious( P 0.01).In men,the immature reticulocyte fraction(IRF) was 0.14± 0.06 ;in women,the IRF was 0.13±0.05.The difference was not obvious( P 0.05).Conclusions:Reference limits of the reticulocyte counts(percentage,absolute value and immature reticutocyte fraction),according to sex and analysis,are provided.
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