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《Journal of Bengbu Medical College》 2004-03
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Apoptosis of γδT cells and αβT cells induced by ceramide

MEI Chuan zhong,SHEN Ji long,CHEN Yong,LI Bai qing (Department of Microbiology and Parasitology,Anhui Medical University,Hefei,Anhui 230032,China)  
Objective:To investigate the role of apoptosis of γδT cells and αβT cells induced by ceramide.Methods:The peripheral blood mononuclear lymphocytes of healthy donors were stimulated respectively with the low molecular peptide antigen derived from Mycobacterium tuberculosis antigen(Mtb Ag) and CD3mAb to generate Mtb Ag activated T cells(γδT cells were predominent) and CD3mAb activated T cells(αβT cells were predominent).These two T cell subsets were treated with Ceramide(C2 Cer) at different concentration;after 3hours of cultivation,the apoptosis of different subsets of T cells was measured by flowcytometry(FCM),using Annexin V FITC/PI staining;and the availability of T cells was detected by methl thiazolyl tetrazolium(MTT) colorimetric assay.Results:The percentage of γδT cells in Mtb Ag activated T cells and αβT cells in CD3mAb activated T cells were 70% to 90% and 90%,respectively.The apoptosis and the inhibition of the availability of γδT cells and αβT cells induced by lower concentration of C2 Cer(≤10 μmol/L)appeared weak in contrast to their control.There were significant differences in the role of apoptosis of γδT cells and αβT cells induced by higher concentration of C2 Cer;the percentage of the apoptotic γδT cells and αβT cells induced by C2 Cer(100 μmol/L) increased by 10.4%( P 0.05 ) and 73.5%( P 0.01) in contrast with their control,and the percentage of cells in the region live cells(R1) was 88.1%( P 0.05) and 7.8%( P 0.01) of the ones of their control;whereas the percentage of the apoptotic γδT cells and αβT cells induced by C2 Cer(500 μmol/L) increased by 71.9% and 73.8%( P 0.01) in contrast with their control,and the percentage of cells in R1 was 36.5% and 2.7%( P 0.01) of the ones of their control.Conclusions:The apoptosis of γδT cells and αβT cells can be induced by ceramide;it needs higher concentration of C2 Cer to induce the apoptosis of γδT cells than to induce the apoptosis of αβT cells.
【Fund】： 国家自然科学基金 (NO3 0 0 70 72 1)资助项目
【CateGory Index】： R392
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