Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Bengbu Medical College》 2004-03
Add to Favorite Get Latest Update

Apoptosis of γδT cells and αβT cells induced by ceramide

MEI Chuan zhong,SHEN Ji long,CHEN Yong,LI Bai qing (Department of Microbiology and Parasitology,Anhui Medical University,Hefei,Anhui 230032,China)  
Objective:To investigate the role of apoptosis of γδT cells and αβT cells induced by ceramide.Methods:The peripheral blood mononuclear lymphocytes of healthy donors were stimulated respectively with the low molecular peptide antigen derived from Mycobacterium tuberculosis antigen(Mtb Ag) and CD3mAb to generate Mtb Ag activated T cells(γδT cells were predominent) and CD3mAb activated T cells(αβT cells were predominent).These two T cell subsets were treated with Ceramide(C2 Cer) at different concentration;after 3hours of cultivation,the apoptosis of different subsets of T cells was measured by flowcytometry(FCM),using Annexin V FITC/PI staining;and the availability of T cells was detected by methl thiazolyl tetrazolium(MTT) colorimetric assay.Results:The percentage of γδT cells in Mtb Ag activated T cells and αβT cells in CD3mAb activated T cells were 70% to 90% and 90%,respectively.The apoptosis and the inhibition of the availability of γδT cells and αβT cells induced by lower concentration of C2 Cer(≤10 μmol/L)appeared weak in contrast to their control.There were significant differences in the role of apoptosis of γδT cells and αβT cells induced by higher concentration of C2 Cer;the percentage of the apoptotic γδT cells and αβT cells induced by C2 Cer(100 μmol/L) increased by 10.4%( P 0.05 ) and 73.5%( P 0.01) in contrast with their control,and the percentage of cells in the region live cells(R1) was 88.1%( P 0.05) and 7.8%( P 0.01) of the ones of their control;whereas the percentage of the apoptotic γδT cells and αβT cells induced by C2 Cer(500 μmol/L) increased by 71.9% and 73.8%( P 0.01) in contrast with their control,and the percentage of cells in R1 was 36.5% and 2.7%( P 0.01) of the ones of their control.Conclusions:The apoptosis of γδT cells and αβT cells can be induced by ceramide;it needs higher concentration of C2 Cer to induce the apoptosis of γδT cells than to induce the apoptosis of αβT cells.
【Fund】: 国家自然科学基金 (NO3 0 0 70 72 1)资助项目
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 MEI Chuan-zhong1, LI Bai-qing2 (1. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu 233003, China; 2. Department of Immunology);Effect of protein kinase C on the apoptosis of human γδT cells induced by My-cobacterium tuberculosis antigens[J];;2005-06
2 MEI Chuan-Zhong, LI Bai-Qing, SHEN Ji-Long. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu 233003, China;Role of p38 and phosphatidylinositol 3 kinase(PI3K) in the signal transduction of apoptosis of activated human γδT cells induced by restimulating with Mycobacterium Tuberculosis antigen[J];Chinese Journal of Immunology;2006-03
【Citations】
Chinese Journal Full-text Database 3 Hits
1 HU Jian guo,HOU Yan qiang,CHEN Yong,MIN Hong lin,SONG Xiu yu,LI Bai qing (Department of Microbiology,Bengbu Medical College,Anhui 233003 ,China);Comparison of proliferation capacity and cytotoxicity of MtbAK,CD3AK and LAK cells in vitro[J];Journal of Bengbu Medicinae College;2001-05
2 ;Role of ceramide in the activation and apoptosis of γδT cells induced by low molecular peptide antigen of Mycobacterium tuberculosis[J];Chinese Journal of Immunology;2000-12
3 GAO Xue ping, SONG Xiu yu, CHEN Yong, et al. Department of Oncology, Bengbu Medical College, Bengbu Anhui, 233004 China;INVESTIGATION TO CYTOTOXIC ACTIVITY OF γδT LYMPHOCYTES[J];Tumor;2001-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHU Yong_mei,ZHONG Jin_yi ˇ ,ZHANG Li_hua (Institute of Nutrition,Medical College of Qingdao University,Qingdao266021,Shandong,China);The Effect of HPP on Abnormal Expression of Mice Hepatic Cells POD and Caspase-3[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-04
2 ZHU Guo-qi,TANG LI-jian,WANG Lei,SU Jing-jing,WANG Ning,PENG Dai-yin,LI Qing-lin(Anhui College of Traditional Chinese Medicine & Anhui Key Laboratory of Modern Chinese Materia Medica,Anhui Hefei 230038,China);Study on Baicalin Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231[J];Journal of Anhui Traditional Chinese Medical College;2008-02
3 ZHAO Xin, SONG Jin dan*Key Laboratory of Cell Biology,Ministry of Public Health of China,China Medical Unviersity,shenyang 110001,P.R.China;Clotrimazole Induced Apoptosis in Human Colon Cancer Cell Line CCL229[J];Chinese Journal of Cancer;2002-06
4 ZHANG Ming sheng1*, YUAN Hong yin1, XIONG Bin1, DENG Qin1, TANG Zhi jiao2, XIA Dong21.Department of Oncology, Zhongnan Hospital of Wuhan University,Wuhan 430071, P.R.China2.Department of Pathology,Medical College, Wuhan University, Wuhan 430071,;Expression and Clinical Significance of IGF-Ⅱ mRNA and Bcl-2 Protein in Colorectal Adenocarcinomas[J];Chinese Journal of Cancer;2002-11
5 Li Jin Quan1,Chen Rui Chuan2,Cai Ke Xia2,Ye Zhi Yun11.Department of Biology,2. Cancer Research Center,Life Science School of Xiamen University, Xiamen, Fujian, 361005, P.R.China;Apoptosis of Human Gastric Cancer Cell Induced by Photochemical Riboflavin[J];Chinese Journal of Cancer;2003-03
6 LIANG Tong, CHEN Mei-Jun, ZHOU Ke-Yuan, TANG Xu-Dong, WANG Xu-Guang Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang,Guangdong,524023, P.R.China;Induction of Apoptosis in Human Nasopharyngeal Carcinoma Cell Line CNE-2Z by Curcumin[J];Chinese Journal of Cancer;2004-12
7 YAN Xiao-Jian1,2, LIANG Li-Zhi1,2, ZENG Zong-Yuan1,3, SHI Zhi1,4, FU Li-Wu1,41. State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510060, P. R. China2. Department of Gynecology, Cancer Center,Sun Yat-sen University,Guangzhou, Guangdong, 510060, P. R. China2. Department of Head and Neck Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China3. Research Department, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P. R. China;Effect of Survivin shRNA on Chemosensitivity of Human Ovarian Cancer Cell Line OVCAR3 to Paclitaxel[J];Chinese Journal of Cancer;2006-04
8 HOU Yan qiang,WU Jun ying,HU Jian guo,LI Bai qing (Department of Immunology,Bengbu Medical College,Anhui 233003 ,China);Detection of intracellular cytokines in activated γδT lymphocytes by flow cytometry[J];Journal of Bengbu Medicinae College;2002-05
9 ZHANG Hai feng,LU He zuo,LI Bai qing (Department of Immunology,Bengbu Medical College,Anhui 233003,China);Clone and expression of human granulysin cDNA in E.coli[J];Journal of Bengbu Medical College;2003-04
10 CHEN Li-wen,ZHU An-you,LI Bai-qing(Department of Immunology,Bengbu Medical College,Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College,Bengbu 233003,China);Expression patterns of IL-2 and IFN-γ of activated γδT cells from human peripheral blood[J];Journal of Bengbu Medical College;2005-05
China Proceedings of conference Full-text Database 2 Hits
1 Yang Ruirui Feng Feng Guo Hongyu (Beijing Sport University,Beijing 100084);Ubiquitin Proteasome Pathway and Skeletal muscle Apoptosis in Exercises[A];[C];2007
2 XIE Jian-qun Shanghai University of Traditional Chinese Medicine(Shanghai 201032);Ulcerative colitis TCM treatment based on syndrome differentiation And research progression of Qing Chang Shuan in treating ulcerative colitis[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 9 Hits
1 YUAN Ping, OUYANG Xing-Juan , TAN Lin, DAI Guo-xiang, LUO Dian-xi (1. Department of psychiatry, Anning Hospital of Guizhou, Guiyang 550004, China; 2. Department of laboratory, Anning Hospital of Guizhou, Guiyang 550004, China; 3. Department of laboratory, The Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China);An Investigation of Immunological Function in Alcoholism Patients[J];Journal of Guiyang Medical College;2003-02
2 Wang Houping,Ma Baoli,Han Ziying,Guo Xuecui(Department of Immunology,Shanghai Second Medical University Shanghai Institute of Immunology,shanghai 200025);The Mechanism of Induction of Anergic Cells by CTLA4Ig[J];SHANGHAI J.OF IMMUNOLOGY;2000-03
3 MEI Chuan-zhong1, LI Bai-qing2 (1. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu 233003, China; 2. Department of Immunology);Effect of protein kinase C on the apoptosis of human γδT cells induced by My-cobacterium tuberculosis antigens[J];;2005-06
4 Guan Zengwei, Li Yong, Wang Shenglan, Yang Yepeng, et al. Medical Analysis Center,Beijing Medical University, Beijing 100083,China;Ethanol induced apoptosis in human HL 60 cells[J];JOURNAL OF HYGIENE RESEARCH;2000-01
5 Mei Chuanzhong, Shen Jilong, Chen Yong et al (Dept of pathology, Anhui Medical University, Hefei 230032);Effects of ethanol and dimethyl sulfoxide on the apoptosis and the availability of γδT cells and γδT cells[J];Acta Universitis Medicinalis Nahui;2004-03
6 WU Jun-ying*, LI Bai-qing, HOU Yan-qiang, CHEN Yong, ZHANG Xue-guang. *Department of Immunology, Bengbu Medical College, Bengbu 233003, China;Role of phosphatidylinositol 3 kinase (PI3K) in the intracellular signaling transduction pathways mediated by TCR and CD28 of γδT cell[J];Chinese Journal of Microbiology and Immunology;2003-02
7 ;Role of ceramide in the activation and apoptosis of γδT cells induced by low molecular peptide antigen of Mycobacterium tuberculosis[J];Chinese Journal of Immunology;2000-12
8 HOU Yan-Qiang,LI Bai-Qing,HU Jian-Guo et al.Department of Immunology, BengBu Medical College, Bengbu 233003,China;The Ras/Erk pathway in the specially activated human γδT cells by Mycobacterium tuberculosis low molecular peptide antigen[J];Chinese Journal of Immunology;2003-08
9 GAO Xue ping, SONG Xiu yu, CHEN Yong, et al. Department of Oncology, Bengbu Medical College, Bengbu Anhui, 233004 China;INVESTIGATION TO CYTOTOXIC ACTIVITY OF γδT LYMPHOCYTES[J];Tumor;2001-02
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 MIN Hong lin,SONG Xiu yu,CHEN Yong,LI Bai qing (Department of Microbiology,Bengbu Medical College,Anhui 233003 ,China) [;Anti-tumor cytotoxicity of human γδ?T cells activated by the antigen of Mycobacterium tuberculosis[J];JOURNAL OF BENGBU MEDICINAE COLLEGE;2000-05
2 Li Xinyan, Zhang Xueguang, Xie Wei, et al. (Suzhou medical College,Suzhou 215007);Studies on the Molecular Recognition of Tumor Autigens by γδ T Cells[J];Shanghai J.of Immunology;1997-04
3 Zhang Cai, Tian Zhigang, Zhang Jianhua, et al. (Shangdong Cancer Biotherapy Center, Shandong Academy of Medical Science, Jinan 250062);Comparison on the in vitro Proliferative Abilities and Tumoricidal Activities of LAK, CD3AK and PHA-LAK Cells[J];Shanghai J.of Immunology;1998-04
4 He Wei,Lin Ying,Li Gang,et al.Institute of Basic Medicine Chinese Academy of Medical Sciences & School of Basic Medicine,Peking Union Medical College,Beijing 100005;Effects of microbial antigens on the cytotoxicity and cytokine production of γδ T cells[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1998-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved