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《Journal of Bengbu Medical College》 2006-02
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The applying of the flow cytometer UF-100 in screening asymptomatic urinary tract infection in early pregnancy

LIN Ping~1,SUN Yang~2,ZHANG You-ling~2,LI Feng-yun~3 (1.Department of Clinical Laboratories,2.Labratory of Bacteria,Minghang District Centreal Hospital,Ruijin Hospital Group,Shanghai 201100;3.Department of Microbiology and Parasitology,Bengbu Medical College,Bengbu 233030,China)  
Objective:To study the reliability of flow cytometer UF-100 in detecting asymptomatic urinary tract infections(AUTI) in early pregnancy.Methods:Three-hundred and nine fresh midstream clean-catch urine sample were collected in accordance with standard guideline.10 μl of cleancatch midstream urine specimen was examined by cultures(colony counts of ≥10~() CFU/ml) the other portion was tested with UF-100 for presence of bacteria count and white blood cell.Results:Twenty-one patient(6.8%) had cultures of 10,000 or more colony-forming units of a uropathogen per milliliter.The sensitivities of the UF-100 test in identifying patients with positive cultures was 71.4%,and the specificities was 94.1%.Six patients(28.6% 6/21) had negative UF-100 screening tests but positive urine cultures,and other seventeen patients had positive UF-100 screening tests but negative urine cultures,The positive predict value(PPV) of urine UF-100 evaluation was 46.9%.There was a significant difference in positive rate between the two methods(P0.05),but there was a significant correlation in test results between the two methods(P0.005).Conclusions:Flow cytometer UF-100 tests are not sufficiently sensitive to be of use in the screening for AUTI and therefore many patients would be missed.Bacteriuria culture must be done in diagnosing AUTI.
【Fund】: 上海市闵行区科委资助项目(编号03-119)
【CateGory Index】: R714.2
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