Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Bengbu Medical College》 2006-04
Add to Favorite Get Latest Update

The effects of sodium butyrate on proliferation and apoptosis of esophageal carcinoma Eca-109 cells

SHEN Rong,TAO Yi-sheng (Department of Pathology,Bengbu Medical College,Bengbu 233030,China)  
Objective:To investigate the effects of sodium butyrate(NaB) on proliferation and apoptosis of esophageal carcinoma Eca-109 cells.Methods:Eca-109 cells was treated with NaB in concentration of 1,2.5,5 mmol/L.Morphological changes of Eca-109 cells were observed by inverted microscope.The morphology of apoptotic cells was investigated by transmission electronic microscope.The inhibition rate of cell growth was assessed by MTT assay.TUNEL assay was used for estimating apoptotic rate.Results:Treatment of Eca-109 cells with NaB resulted in significant inhibition of cell growth in time-dependent and dose-dependent manners.Cells became small and round,many of them fell off from the wall under light microscopy.NaB could reduce the solidity of nuclei,make the chromatin condense and get together into karyotheca.NaB inhibited the growth rate of cells obvioursly,the growth rates of cells treated with NaB in concertrations of 1,2.5,5 mmol/L for 24 h,48 h and 72 h were 13.38%,34.15%,39.02%;14.04%,33.33%,46.49%;and 31.11% ,40.74%,48.15%,respectively(P0.05-P0.01).The rates of cell apoptosis in the group treated with 2.5 mmol/L NaB for 24 h and control group were 5.5% and 1.6%,respectively(P0.05).Conclusions:Sodium butyrate could inhibit the proliferation and promote the apoptosis in esophageal carcinoma Eca-109 cells in time-dependent and dose-dependent.
【Fund】: 安徽省教育厅自然科学研究资助项目(2002kj209)
【CateGory Index】: R735.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 CAI Dong-hong(Department of Obstetrics and Gynecology,Affiliated Hospital of Guangdong Medical College Zanjiang 524001,China);Growth-inhibiting effects of sodium butyrate on human chorionic carcinoma JAR cells and mechanism[J];Journal of Hainan Medical College;2008-06
2 XIE Jing,et al (Department of Gastroenterology,the Second Affiliated Hospital,Chongqing Medical University);Effects of trichostatin A(TSA) on the proliferation and apoptosis of hepatoma cell lines and the the replication of hepatitis B virus(HBV)[J];Journal of Chongqing Medical University;2008-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Shu-kui , ZHOU Zhen-ying, WANG Zi-zheng, et al. (Central Laboratory of Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing 210006, China);An Improved Classification Method for DNA Ploidy and Its Clinical Application[J];Labeled Immunoassays and Clinical Medicine;2005-02
2 DI Qian 1, LI Yang 1, ZHAO Xue-jian 1 , MIYAGI Taeko 2 (1. Department of Pathophysiology , School of Basic Medical Sciences,Jilin University,Changchun 130021, China;2. Miyagi Prefectural Cancer Center,Miyagi 981-1293,Japan);Effects of sodium butyrate on cell growth inhibition and apoptosis in PC-3M cell lines[J];Journal of Jilin University of (Medicine Edition);2004-05
3 XU Qin (Department of Gastrointestinal Surgery,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China);Metabolism and application of short chain fatty acids in intestinal surgery[J];PARENTERAL & ENTERAL NUTRITION;1999-04
4 LI Kezhou, LI Ling, LI Jieshou, BAO Yang, LI Yousheng (Research Institute of General Surgery Jinling Hospital,Nanjing 210002,Jiangsu,China);The effect of short-chain fatty acids(SCFA) on graft morphology and absorption after rat small bowel transplantation[J];PARENTERAL & ENTERAL NUTRITION;2000-03
5 YAN Min 1 , CHEN Er-zhen 2 , CAO Wei-xin 2 ,FEI Xu-feng 2, CHEN Xue-hua 3 ( 1. Department of Surgery; 2. Department of Clinical Nutrition, Ruijin Hospital Affiliated to Shanghai Second Medical University; 3. The Institute of Digestive Surgery of Shanghai, Shanghai 200025, China);Experimental study on the effects of butyrate on the apoptosis of colon cancer cell line[J];Parenteral & Enteral Nutrition;2004-05
6 Ji Qunsheng and Chen Hanyuan Department of Biology;Preliminary studies on effects of sodium butyrate on ultrastructure of human gastric adenocarcinoma cell line[J];Journal of First Military Medical University;1991-04
7 LI Fu-yan~(1),CHEN Yao-xing~(1),WANG Zi-xu~(1),WANG Qing-li~(1),GUO Yu-ming~(2),ZHANG Long-chao~(2)(1.College of Veterinary Medicine,China Agricultural University,Beijing,100094,China;2.College of Animal Science and Technology,China Agricultural University,Beijing,100094,China);Effect of Three Feed Additives on Number of Mast Cells in Small Intestine of Broiler[J];Progress in Veterinary Medicine;2006-02
8 Li Peiguo (Dept of Animal Science,HVTTC,Changli,066600);CHICKEN COCCIDIOSIS AND ITS CONTROL(SUMMARY)[J];JOURNAL OF HEBEI VOCATION-TE CHNICAL TEACHERS COLLEGE;2000-01
9 Zhang Litian, Yao Yongming, Lu Jiaqi et al. Burns Institute, 304 Div ision, General Hospital of PLA(Formerly 304 Hospital of PLA), Beijing 100037, Ch ina;Antagonistic effect of sodium butyrate against high mobility group box 1 protein in rats with sepsis[J];Medical Journal of Chinese People's Liberation Army;2005-10
10 SHI Song-lin~(1,2),WANG Yong-yu~(1,2),LIANG Ying~(1,2),LI Qi-fu~(1,2*)(1.Laboratory of Cell Biology,School of Life Science;2.the Key laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University,Xiamen361005,China);EFFECTS OF TACHYPLESIN AND N-SODIUM BUTYRATE ON THE MORPHOLOGY AND ULTRASTRUCTURE CHANGES OF HUMAN GASTRIC ADENOCARCINOMA CELLS LINE BGC-823[J];Acta Anatomica Sinica;2006-03
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 WANG Li,LIU Xiao-yan,GONG Shu,et al.(Laboratory of Medical Molecular Biology,Luzhou Medical College,Luzhou,Sichuan 646000,China);Effect of TSA on the expression of FHIT in the human hepatoma cell line HepG2[J];Chongqing Medicine;2010-15
2 CAI Dong-hong(Department of Obstetrics and Gynecology,Affiliated Hospital of Guangdong Medical College Zanjiang 524001,China);Growth-inhibiting effects of sodium butyrate on human chorionic carcinoma JAR cells and mechanism[J];Journal of Hainan Medical College;2008-06
3 Liu Xiaoyan,et al The Laboratory of Molecular Biology of Luzhou Medical College;PROTEOMIC ANALYSIS OF HEPG2 STIMULATED WITH TRICHOSTATIN A[J];Journal of Luzhou Medical College;2011-02
4 HUANG Gang-ya,WANG Li,DUAN Cheng-gang,et al.Leshan Vocational and Technical College,Leshan 614000 China;Effect of Histone Deacetylase Inhibitor TSA on Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell Line HepG2 and the Study of Its Mechanism[J];Chinese General Practice;2010-30
5 Wen Qiao, Xiao-Dan Yang, Dan Wang, Department of Gastroenterology, the First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China;Trichostatin A inhibits the growth of human gastric cancer SGC-7901 cells[J];World Chinese Journal of Digestology;2009-35
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved