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《Journal of Bengbu Medical College》 2009-07
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Analysis on application in the crossmatching of sustainable hemodialysis' patients by microcolumn gel test and manual polybrene test

CAO Rui-hua1,HU Dao-jun2,WANG Li1,WEI Xi3(1.Department of Blood Transfusion,Fuyang Second People's Hospital,Fuyang Anhui 236015;2.The Teaching and Research Section,Department of Biochemistry and Molecular Biology,Anhui Medical University,Hefei Anhui 230032;3.Academy of Life Science and Technology,China Pharmaceutical University,Nanjing Jiangsu 210038,China)  
Objective:To compare irregular antibodies' postive rates of the first and repeated blood transfusions,and to approach applied value of the crossmatching test by microcolumn gel test(MGT)and manual polybrene test(MPT)in the sustainable hemtodialysis' patients.Methods:Ninety-six patients sustainable hematodialysis' who required blood transfusion were crossmatched by MGT and MPT.Results:In 462 cross matching tests for 96 patients,the difference of crossmatching compatibility showed statistical significance between MGT and MPT(P0.01).Among the total,62 cases of MGT major and minor incompatibility were positive by direct antiglobulin test.Moreover,with the increase of transfusion'times,the positive rate of irregular antibodies also increased by MGT and MPT(P0.01).The total positive rate was higher using MGT than using MPT,the susceptibility of MGT was obviously higher than MPT(P0.01).Conclusions:In sustainable hemtodialysis'patients,there will be line irregularity of antibodies after continual and repeated transfusions.In MGT,sensitized RBC will lead to difficulty of crossmatching test.In combination with MPT,we can further understand the nature of incompatibility,which can improve safety of transfusion.It might be of good complementarity when there is minor incompatibility of MGT in cross matching.
【CateGory Index】: R459.5
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Yi,LIU Yanchun,LIU Hongli,et al.(Jiangsu Province Blood Center,Nanjing 210042,China.);Application of microcolum gel test for serodiagnosis of hemolytic disease of newborn[J];Journal of Clinical Laboratory Science;2005-01
2 MA Shu Xuan, LIU Jing Han, LI Xi Jin, LUO Qun, CHEN Min Cai, WANG Hai Jun Department of Blood Transfusion, General Hospital of PLA, Center of Clinical Transfusion of PLA, Beijing 100853, China;Micro-Column Gel Indirect Anti-globulin Technique for Screening and Identification of Irregular Antibody[J];Journal of Experimental Hematology;2003-02
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Yi,LIU Yanchun,LIU Hongli,et al.(Jiangsu Province Blood Center,Nanjing 210042,China.);Application of microcolum gel test for serodiagnosis of hemolytic disease of newborn[J];Journal of Clinical Laboratory Science;2005-01
2 MA Shu Xuan, LIU Jing Han, LI Xi Jin, LUO Qun, CHEN Min Cai, WANG Hai Jun Department of Blood Transfusion, General Hospital of PLA, Center of Clinical Transfusion of PLA, Beijing 100853, China;Micro-Column Gel Indirect Anti-globulin Technique for Screening and Identification of Irregular Antibody[J];Journal of Experimental Hematology;2003-02
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