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《JOURNAL OF BENGBU MEDICINAE COLLEGE》 1998-01
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Influence of extracellular matrix on adhesive function of endothelial cell

Zhou Xulong,Deng Yiping(Department of Pathology,Medical College of Jinan University,Guangzhou 510632)  
Objective:To promote the adhsion of endothelial cells onto polyurethane (PU) material for realization of endothelialization on artificial heart and blood vessel.Methods:The influence of elements in extracellular matrix on the adhesive function of endothelial cells onto the PU material was observed with fluorescent method.Results:It revealed that collagen I,collagen Ⅳ,FN,VN precoated on the PU material could significantly enhance the adherance of endothelial cell,among which VN seemed to be the best.In concentration of 50 mg/L the cell attachment rate in collagen I,collagen Ⅳ,FN,VN group after 15 minutes contact were 32.31%,63.77%,54.43%,42.34%(P0.05) and 27.73%(P0.01),respectively.Conclusions:It suggests that precoating of PU material with VN or with VN and other ECM elements in combination could be lead to endothelialization.
【CateGory Index】: R329.24,R313.11
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