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Effect of macrophage conditioned media on the neurites outgrowth in ventral part of the spinal cord

Zhao Li,Qin Jianqiang,Chen Shiwen (Department of Anatomy,Bengbu Medical College,Anhui 233003 )  
Objective:To provide data for treatment of peripheral nerve injury.Methods:The effect of macrophage conditioned mediums on neurites outgrowth in the ventral part of the spinal cord was observed with culturation and AChE histochemcal staining.Results:Macrophage conditioned media could promote the neurites outgrowth of the spinal ventral neuron.The length of outgrowth was 145.90±31.48 μm in 6 days culture,in experimental group and 60.44±16.66 μm in control group( P 0.001).Conclusions:If the microenvironment is infiltrated with macrophage artificially or with macrophage derived neurotrophic factor in peripheral nerve injury,especially old injury,motor nerve regeneration will be promoted.
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