EFFECTS OF ELECTROACUPUNCTURE AND OF INCUBATION WITH SUBSTANCE P OR 5-HYDROXYTRYPTAMINE IN VITRO ON LYMPHOCYTE RECEPTORS OF SUBSTANCE P AND 5-HYDROXYTRYPTAMINE
Liu Xiaoping, Wu Jinglan, Yu Yejun Department of Histology and Embryology
By means of immune gold -silver staining (IGSS),we studied the effects of electroacupuncture (Ea) and incubation with Substance P (SP)or 5-Hydroxytryptamnine (5-HT)on the lymphocyte receptors of SP and 5-HT. we found that there were 5-HT and SP receptors located on lymphocytes and that IGSS dark brown granules were distributed on the surface of lymphocytes, either scattered or crowded in cap-like groups. Ea or incubation with SP or 5-HT could increase IGSS reactions significantly. This suggests that Ea or incubation with SP or 5-HT may enhance the activity of the unbound SP and 5-HT receptors (P0.05). Since Naloxone(Nx) is an opioid like peptide receptor antagonist, the reverse effects of Nx may indicate that either under incubation with SP or with 5-HT in vitro their effects on lymphocytes receptors may be mediated by opioid like peptide receptors.