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《Acta Acadimae Medicinae Qingdao》 2003-01
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CONSTRUCTION OF RECOMBINANT CLONE OF SITE-SPECIFIC GENE MUTANT OF HUMAN INTERLEUKIN-2

HOU Wei,QIAN Dongmeng, YAN Zhiyong, et al\ Department of Microbiology, Qingdao University Medical College, Qingdao 266021\;  
Objective\ To construct recombinant clone of site specific mutagenesis(88ArgIL 2) of human interleukin 2(IL 2) gene.\ Methods\ Nested PCR was applied to amplify gene coding for IL 2 from PHA stimulated human tonsil cell cDNA. Then the specific primers containing mutated base were synthesized to obtain the target fragments by means of recombinant PCR. The amplified fragments were cloned to pGEM T Easy vector and transformed into E.coli JM109. The positive clone was obtained by blue/white screening. \ Results\ The mutated IL 2 gene clone was obtained successfully. \ Conclusion\ The construction of the recombinant clone has laid a foundation for its prokaryotic and eukaryotic expressions and preparing a low toxic, high efficient drug.
【Fund】: 山东省自然科学基金重点资助项目 (E99C0 2 )
【CateGory Index】: Q784
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