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《Acta Acadimae Qingdao Umvesitatis》 2005-01
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ESTABLISHMENT OF MODEL IN DIABETES MELLITUS COMPLICATED WITH INFECTION AND CHANGES OF TUMOR NECROSIS FACTOR α IN RATS

GUO Jian, SONG Da-lin, WANG Yan-ming (Laboratory of Endocrinology, Qingdao Hiser Medical Group, Qingdao 266033, China);  
Objective To establish a rat model of diabetes mellitus(DM) complicated with infection, detect the changes of tumor necrosis factor α(TNFα) mRNA and protein in pancreas, and assess the inflammatory changes in this condition. Methods The rat model with diabetes mellitus complicated with infection was obtained by STZ-injection and CLP-operation. The rats were divided into normal, sham-operated, DM, infection, and DM complicated with infection(DMI) groups. The C-reactive protein(CRP), insulin and TNFα were measured by ELISA; semi-quantitative TNFα mRNA in pancreas was detected by RT-PCR. Results The rat models were established successfully. The levels of TNFα mRNA in pancreas and TNFα of plasma were higher than those in the normal, sham-operated and DM groups (H=7.56,q=3.25-5.26, P0.05;F=9.49, q=3.76-12.92,P0.05, 0.01),but not significantly different with those in the infection group (P0.05). The concentration of TNFα in the pancreas increased significantly in the DMI group (F=7.78,q=2.80-4.32,P0.05). Conclusion DM complicated with infection extended the inflammation reaction. The level of TNFαmay reflect the pancreatic injuries.
【CateGory Index】: R587.1
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【References】
Chinese Journal Full-text Database 1 Hits
1 Yu Dong,Song Dalin,Li Mei Department of Cardiology Qingdao Municipal Hospital,Qingdao 266011,China;Change of mRNA and protein expression of monocyte chemoattractant protein-I on patients with early diabetes mellitus[J];Medical Laboratory Science and Clinics;2006-05
【Citations】
Chinese Journal Full-text Database 2 Hits
1 YANG Yi,QIU Haibo,ZHOU Shaoxia,XU Hongyang,LIU Shaohua,ZHENG Ruiqiang,HUANG Yingzi. Department of Critical Care Medicine,Zhongda Hospital and School of Clinical Medicine,Southeast University, Nanjing 210009,Jiangsu China;Effect of inhaled nitric oxide on pulmonary inflammatory response in mice with acute lung injury[J];Chinese Critical Care Medicine;2002-12
2 SONG Da-lin1, MEI Shao-ping1, DONG Yan-hu1, Herbert Spapen2. 1. Department of Molecular Biology, Hiser Medical Center of Qingdao, Qingdao 266033, Shandong; China; 2. Intensive Care Department, Academic Hospital, Free University of Brussels, Belgium;Influence of catecholamines on myocardium in experimental septic shock[J];Chinese Critical Care Medicine;2003-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Li-fang1,SHAN Bao-en2,SHAN Tie-qiang2(1.Laboratory Department of the Second Hospital of Hebei Medical University,Shijiazhuang 050000;2.Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China);Effects of triterpenes compound of cortex periplocae on regulatory T cells function in N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in F344 rats[J];Carcinogenesis,Teratogenesis & Mutagenesis;2010-03
2 WANG Xing-man TANG Ren-shu YU Han-yang ZHAO Jun WANG Ming-li (Department of Microbiology,Anhui Medical University,Key Laboratory of Gene Resource Utilization for Genetic Diseases,Ministry of Education and Anhui Province,Hefei Anhui 230032);Potency Comparison of Recombinant Porcine Interferon-α by Two Kinds of Cells[J];;2009-24
3 HOU Wei,QIAN Dongmeng, YAN Zhiyong, et al\ Department of Microbiology, Qingdao University Medical College, Qingdao 266021\;;CONSTRUCTION OF RECOMBINANT CLONE OF SITE-SPECIFIC GENE MUTANT OF HUMAN INTERLEUKIN-2[J];Acta Acadimae Medicinae Qingdao;2003-01
4 MENG LIN,LIU MING-JUN,WANG BIN, et al(Department of Microbiology,Qingdao University Medical College,Qingdao 266021,China);CLONING AND PROKARYOTIC EXPRESSION AND ACTIVITY OF ~(88)Arg IL-2[J];Acta Academiae Medicinae Qingdao Universitatis;2006-04
5 CHEN Li-Long,ZHU Xiao-Tong,JIANG Qing-Yan **,GAO Ping,SHU Gang,FU Wei-LongCollege of Animal Science, South China Agricultural University, Guangzhou 510642, China;Effects of synthetic bursin on proliferation of chicken splenic lymphocytes and expression of IL-2 and IL-6[J];Acta Zoologica Sinica;2006-01
6 WT5”BZ] LIU Xiao yong et al.Faculty of Stomatology,Capital University of Medical Science,Beijing 100050 [WT5”HZ];EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION AND ALKALINE PHOSPHATASE EXPRESSION OF HUMAN PERIODONTAL LIGAMENT CELLS.[J];Beijing Journal of Stomatology;2001-02
7 CHEN Cui-li~1,SHI Ding-ji~2(1.College of Arts and Science of Beijing Union University,Beijing 100083,China;2.China Institute of Botany,The Chinese Academy of Science, Beijing 100093,China);Construction of Anabaena Transferred the Gene Encoding the Human Granulocyte Colony-stimulating Factor[J];Journal of Beijing Union University;2005-03
8 CHEN Su-juan, DONG Xiao-jun, SUN Xiao-li, et al. (Centre of Science and Technique Development, General Hospital of PLA, Beijing 100853, China);Establishment and Primary Application of Interleukin-4 Radioimmunoassay[J];Labeled Immunoassays and Clinical Medicine;2002-01
9 NIU Ying-peng(College of Physical Education Science of Henan University,Kaifeng 475001,Henan China);The Effect of EGb on the Activities of CK,LDH and IL-4 in Blood of Rats[J];Journal of Beijing Sport University;2006-10
10 Yao Huiqin,Wang Yang,Zheng Jie,et al (Beijing Tongren Hospital,Beijing 100730);Study on levels of IL-8 in serum and urine of patients with acute urinary tract infection[J];Beijing Medical Journal;2001-01
China Proceedings of conference Full-text Database 6 Hits
1 ZHAO Dan-ping1,TAO Bin1,CHEN Li-gong,LI Yan-qin,SONG Qin-ye2,LI Tan-qing,YANG Fang,LI Hong-yuan,YUAN Hong-xin(Experimental Station of Animal Pathogen Biology,Ministry of Agriculture /Agricultural University of Hebei,Baoding Hebei 071000,China);INFLUENCE OF PORCINE CIRCOVIRUS TYPE 2 INFECTION ON IMMUNE RESPONSE OF PIGS TO CLASSICAL SWINE FEVER VACCINE[A];[C];2012
2 Wang Yingnian , Liu Jiahong , Sun Rongli, et al. The Research Institute of Tuberculosis and Respiratory Disease in Qingdao Center Hospital, Qingdao 266042;A series of Studies on Diagnostic Value of Detection of P-selectin, IL-1 α, sFas, sFasL, AM and Combined Detection of CEA in differentiating Tuberculous from Malignant pleural effusions[A];[C];2005
3 CHEN Xiao,CHEN Li,CHEN Ya Qing.YANG Zhen Hua (Department of Gynecology And Obstetrics,The Affiliated Hospital of Medical College of Chinese People's Armed Police Forces,Tianjin 300162,China);Effect of HCG secretion and expression of PCNA and Bcl-2 in trophoblast cells treated with T_(H1)-type/T_(H2)-type cytolines[A];[C];2010
4 LEI Jian-Yong, ZHANG Lian-Feng YANG Jian-Liang, JIN Jian (Department of Pharmaceupical and Molecular Biotechnology, School of Biotechnology, Southern Yangtze University, Wuxi, PR China 214036 );Secretory expression of the fusion protein IFN β-HSA in Pichia pastoris[A];[C];2006
5 ;Experimental study on the effect of cryopreservation on cord immuno-characteristic of cord blood monounclear cells[A];Chinese association of Refrigeration[C];2006
6 ;IL-2穴位注射对佐剂性关节炎大鼠炎症局部POMCmRNA和PENKmRNA表达的影响[A];[C];2007
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YANG Pei ,CHEN Wei ,WEN Ai qing (Department of Laboratory,Daping Hospital,the Third Military Medical University ,Chongqing 400042);The effect of serum lipase combined with CRP, IL-6 in rapid diagnosis and prognosis of patients with acute pancreatitis[J];Chongqing Medical Journal;2002-11
2 WANG Wen-meng,HU Kai-hua, HUANG Jing, et al.Dapartment of Internal Medicine, Hunan Armed Police Force Hospital,Changsha,410006,China;Changes of Chemotactic Factor Monocyte Chemotactic Protein-1 (MCP-1) Expression in Patients with Congestive Heart Failure[J];Journal of Chinese Physician;2003-04
3 XIN Shi-meng 1,LIU Yuan-hong 2,NIE Zhi-yu 1 [WT5BX](1.Department of Neurology,the Second Affiliated Hospital,Dalian Medical University,Dalian 116023,China; 2.Department of Neurology,Puyang People Hospital,Puyang 457000,China). [WT5HZ];The relation of rat MCAO model quality to thread diameter,length and body weight of the rats[J];JOURNAL OF DALIAN MEDICAL UNIVERSITY;2000-02
4 YUAN Xiao-mei,MA Kang-hua(Department of Cardiology,The First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China);IGF-1 and PKC expressions in left ventricular remodeling in rats after myocardial infarction[J];Acta Academiae Medicinae Militaris Tertiae;2006-12
5 ZHENG Hui,YU De-min.Department of Neurology,Metabolic Disease Hospital Affiliated to Tianjin Medical University,Tianjin 300070,China;The pathogenesis and diagnosis of diabetic cardiomyopathy[J];International Journal of Endocrinology and Metabolism;2006-02
6 ZHAO Lin-shuang,XIANG Guang-da,Tang Ying,et al. Department of Endocrinology,Wuhan General Hospital,Guangzhou Military Command,Wuhan Hubei 430070,China;Association of Gln 223Arg Variant in Leptin Receptor Gene in Type 2 Diabetes in Wuhan "Han" Population[J];Military Medical Journal of South China;2008-02
7 YANG Li,WEN Ye,WANG Xiao-kun,et al.Department of Medical Experiment,Wuhan General Hospital,Guangzhou Military Command,Wuhan Hubei 430070,China;Protection Effect of QiLian Soup on T2DM Rats[J];Military Medical Journal of South China;2008-03
8 LI Xia , ZHOU Zhi guang *, QI Hai ying, CHEN Xiao yan, HUANG Gan(Department of Endocrinology, Second Xiangya Hospital, Central South University, Changsha 410011, China);Replacement of insulin by fasting C-peptide in modified homeostasis modelassessment to evaluate insulin resistance and islet β cell function[J];;2004-04
9 Chen Qunli,Yang Wubiao,Ma Lingjun,(Department of Biochemistry and Molecular Biology,Medical College of Henan Science and Technology University,Henan Luoyang471003,China);The establishment of expertimental rat model with diabetic foot[J];Henan Journal of Preventive Medicine;2004-01
10 SONG Lili, FU Jianliang, SHAO Fuyuan Department of Neurology, Changzheng Hospital, Shanghai 200003;Establishment of vascular dementia rat model with diabetes mellitus[J];Journal of Zhengzhou University(Science Medical);2004-06
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 WANG Yu1,NING You1,2,WANG Pei-yu1,3,YANG Yong-qing1.1.Molecular Biology(Acupuncture) Laboratory,Shanghai University of Traditional Chinese Medicine Research Institute of Acupuncture and Meridian,Shanghai 200030,China;2.Fudan University Huashan Hospital Research Institute of Combined Traditional Chinese and Western Medicine,Shanghai 200040,China;3.Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China;Method of Placing a Rat on an Elevated Platform for Acupuncture[J];Shanghai Journal of Acupuncture and Moxibustion;2012-05
2 Guo Yue,Zhang yang,Sun Xiaolei,et al.Tianjin Orthopaedic Hospital,Tianjin(300211),China;Repairing role of Myogenic Elephatis Corium Cream to Ulcer Wound with Tendon Injury Model in Diabetic Rats[J];Chinese Journal of Surgery of Integrated Traditional and Western Medicine;2011-06
3 GUO Jian~* SONG Da-lin KANG Wei-qiang Department of Molecular Laboratory,Qin~dao Blood Center,Qingdao 266071,China;Clinical study on change of chemokine mRNA and protein in patients with diabetes mellitus complicated infection[J];Chinese Journal of Laboratory Medicine;2007-10
4 TAO Mao-can,CAO Yi,XU Jing-lun,HUANG Ping(Zhejiang Chinese Medical Hospital,Hangzhou 310006,Zhejiang,China);The Establishment of Diabetic Foot Rat Model with Blood Stasis Syndrome[J];Chinese Archives of Traditional Chinese Medicine;2009-02
5 Guo Xuejun1,Wang Sicheng2,Zou Yihai1,Zhang Yongbin1,Xie Lingling1(1.Guangzhou University of Traditional Chinese Medicine,Guangzhou,510405,China;2.State Administration of Traditional Chinese Medicine,Beijing,100029 China);Laboratory Animal Model Studies and Analysis of Literature Statistics on Core Journal of Chinese Medicine from 2005 to 2009[J];Chinese Medicine Modern Distance Education of China;2011-12
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Qiu Haibo,Chen Dechang,Pan Jiaqi,et al.Department of Critical Care Medicine,Nanjing Railway Medical College Hospital, Nanjing 210009;Effects of nitric oxide inhibition on inflammatory response in acute lung injury[J];JOURNAL OF EMERGENOY MEDICINE;1999-03
2 QIU Haibo ,ZHOU Shaoxia,CHEN Dechang. Department of Critical Care Medicine,Nanjing Railway Medical College Hospital,Nanjing 210009;Effect of nitric oxide on inflammatory response of alveolar macrophages[J];CHINESE JOURNAL OF ANESTHESIOLOGY;1999-08
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