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《Acta Academiae Medicinae Qingdao Universitatis》 2006-04
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CLONING AND PROKARYOTIC EXPRESSION AND ACTIVITY OF ~(88)Arg IL-2

MENG LIN,LIU MING-JUN,WANG BIN, et al(Department of Microbiology,Qingdao University Medical College,Qingdao 266021,China)  
Objective To construct clone of ~(88)Arg IL-2 and express it in Escherichia coli. Methods Site-specific mutated primers were designed according to natural IL-2 DNA sequence.~(88)Arg IL-2 gene was amplified using PCR.Then the ~(88)Arg IL-2 gene was inserted into expression vector pGEX4T-2 and induced by IPTG to express the protein in E.coli DH5α.(Results The) molecular weight of fusion protein was 42 000.Western blotting test revealed that it had good specificity to anti-IL-2.The result of MTT assay indicated that ~(88)Arg IL-2 could stimulate the proliferation of CTLL-2 which was IL-2-dependent cell strain.(Conclusion ~(88)Arg) IL-2 has been cloned;the fusion protein is expressed in prokaryotic expression system with biologic activity.
【CateGory Index】: Q78
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