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《Acta Academiae Medicinae Qingdao Universitatis》 2009-01
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THE EFFECT OF HIGH-DOSE VITAMIN C ON PERIPHERAL BLOOD CELL ACTIVITY

XUE MEI-LAN,ZHANG XIU-ZHEN,MA AI-GUO,et al (Department of Biochemistry and Molecular Biology,Qingdao University Medical College,Qingdao 266021,China)  
Objective To investigate the effects of high-dose vitamin C(VC) on peripheral blood lymphocyte(PBL) proliferation,anti-DNA oxidative damage activity and erythrocyte membrane fluidity.Methods Fifty Wistar rats were randomly divided into four groups: control group,and groups A,B,and C,which were fed with forage containing 0,2 000,5 000,and 10 000 mg/kg of VC,respectively,for eight weeks.Upon completion of the experiment,the rats were sacrificed and blood samples obtained.The levels of plasma VC,SOD,MDA and membrane GSH-Px were determined.The erythrocyte membrane fluidity was detected by fluorescence polarization method,lymphocyte transformation rate by MTT method and DNA oxidative damage by comet assay.Results Compared with the control group,plasma SOD in group A was significantly increased(F=2.987,q=4.24,P0.05),while MDA decreased(F=4.176,q=4.23,P0.01).The erythrocyte membrane fluidity and lymphocyte proliferation of group A were significantly increased(F=2.278-2.763,q=3.67-3.84,P0.05).The plasma VC and MDA in group C were markedly higher than that for the control(F=3.235,q=4.63,P0.01;q=4.08,P0.05),while SOD and GSH-Px inerythrocyte membrane in group C were significantly lower than the control(q=3.68-4.13,P0.05).The H2O2-induced DNA damages in groups B and C were more serious than that of the control(F=4.137;q=3.94,4.25;P0.05).Conclusion A higher dose(2 000 mg/kg) of VC supplementation can enhance the antioxidative activity in rats and improve erythrocyte membrane fluidity and lymphocyte proliferation.However,the oxidative damage of lymphocyte will be caused instead when the dose of VC exceeds 5 000 mg/kg.
【CateGory Index】: R363
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