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《青岛医学院学报》 1998-04
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THE EFFECT OF LEAD NITRATE ON CONFORMATION AND CATALYTIC ACTIVITY OF HUMAN PLACENTAL ALKALINE PHOSPHATASE

Geng Fangsong,Liu Hongming,Wang Xiuli, et al Department of Biochemistry, Qingdao Medical College, Qingdao 266021  
Objective To study the effect of lead on human placental alkaline phosphatase(PLAP)and discuss the molecular machanism of lead affecting the development of fetus. Methods Spectrophotometry was used to study the activity of PLAP under different concentrations of Pb(NO_3)_2.Fluorometry was used to observe the change of the fluorescence spectra.The inhibition type of Pb(NO_3)_2 on PLAP was determined by using the double reciprocal plot. Results No obvious change of PLAP activity was observed at the concentrations of Pb(NO_3)_2 lower than 0.5mmol/L,whereas its fluorescence emission intensity was slightly increased and its emission maximum was blueshifted.When the concentration of Pb(NO_3)_2 was 0.75mmol/L,both the PLAP activity and emission intensity were rapidly decreased. When the concentration of Pb(NO_3)_2 was higher than 0.75mmol/L, the PLAP activity and emission maximum were gradually decreased, while the emission maximum was less blueshifted.At concentration of 10.00mmol/L, the PLAP activity was completely lost and the fluorescence was quenched. Pb(NO_3)_2 was a uncompetitive inhibitor of PLAP and its inhibition constant (Ki) is 1.40mmol/L. Conclusion The PLAP activity was inhibited and its conformation was changed by Pb(NO_3)_2.This may be one of the important molecular mechanisms that Pb(NO_3)_2 influences the development of fetus. 
【Fund】: 山东省卫生厅科研基金
【CateGory Index】: Q754,R595.2
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