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《Journal of Baoding Teachers College》 2005-02
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Common Methods of Total RNA Isolation from Plant Tissues and Its Optimization Strategies

GU Shou-qin1, XIE Ling-jun2, FAN Yong-shan3 (1.Department of Biology, Baoding Teachers College, Baoding 071000, China;2.Th e Dingzhou branch school of Baoding Teachers College, Dingzhou 073000, China;3. Department of Biology, Tangshan Teachers College, Tangshan 063000, China)  
The common methods and some detection technologies of the total RNA isolation from plant tissues have been summarized. The methods about eliminating some plant tissue components interfering the total RNA isolation, such as pheno lic compound, polysacchrides, lipids, proteins, were also introduced. About how to reduce the decomposition of RNA and the effect of RNase had been illuminated too.
【CateGory Index】: Q943.2
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