Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Baoding Teachers College》 2006-02
Add to Favorite Get Latest Update

A Study for Isolation of Populus Chloroplast and cpDNA

CUI Bin-bin,LI Yun,FENG Da-ling,LIU Ying,FU Xiao-xia(College of Biological Science and Biotechnology,Beijing Foresty University,Beijing 100083,China)  
The method is improved by the use of high salt in combination with low pH buffer to isolate chloroplast and DNA from leaves of populus.The process include whipping,differential centrifugation,cracking and purification.It can be obtained that high quality chloroplast and higher yield 0.64 ug/g cpDNA.The technique is easy to obtain cpDNA with high yields,high purity and high quality,it avoids the time-consuming and expensive process of Cesium Chloridesucrose density gradient centrifugation or sucrose step gradients centrifugation.
【Fund】: 北京市自然科学基金(6042020)
【CateGory Index】: Q94-33
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved