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《Chinese Journal of Virology》 1989-04
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PROMOTER-LIKE FUNCTION OF VACCINIA VIRUS DNA FRAGMENTS IN E.COLI

Tsao Hsu Liu Genqi Zhu Jiming(Chu Chiming) (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing )  
During our study using β-galactosidase (Lac Z) gene as a marker for the selection of recombinant vaccinia viruses, we observed that blue bacterial colonies were formed on agar medium containing X-gal after E. coli was transfected by plasmids containing a vaccinia virus fragment with promoters for 11kd or 25kd proteins inserted upstream of the Lac Z gene.The relative level of β-galactosidase expressed by 11kd and 25kd promoters in E.coli did not run parallel to that expressed in recombinant vaccinia viruses. Lac Z gene inserted into different restriction sites of the F fragment of vaccinia virus DNA may also be expressed to different extent in E. coli,either in one orientation or in both orientations. These results showed that certain vaccinia virus DNA fragments may possess a promoter-like function in E.coli. The possible nature of this phenomenon and suggestions for further studies are discussed.
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【References】
Chinese Journal Full-text Database 3 Hits
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Yuwen Hao Hou Yunde Li Zhiliang Institute of Virology, Chinese Academy of Preventive Medicine, Beijing Department of Biology, Zhong Shan University, Guangzhou;USE OF ESCHERICHIA COLI PLASMID FOR DETECTION AND ISOLATION OF PROMOTERS FROM VACCINIA VIRUS GENOME[J];Chinese Journal of Virology;1987-03
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 Yuwen Hao Hou Yunde(Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);CONSTRUCTION OF A NEW SET OF VACCINIAVIRUS EXPRESSION VECTORS USING ITS HEMAGGLUTININ GENE AS A SELECTION MARKER[J];Chinese Journal of Virology;1988-04
2 Liu Zhewei Wu Shuhua Jin Qi Xue Shuixing Pan Wei Sun Guoxian Hou Yunde National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;A DNA FRAGMENT FROM THE UPSTREAM REGULATING REGION OF HUMAN PAPILLOMAVIRUS TYPE 6b GENOME POSSESSES ENHANCER ACTIVITY IN Escherichia coli[J];Chinese Journal of Virology;1992-03
3 Pan Wei Wu Shuhua Jin Qi Yu Wenhao Liu Zhewei Jin Dongyan Du Ping Hou Yunde (National Laboratory of Molecular Virology and Genetic Enginering, Beijing);STUDY ON THE ENHANCER-LIKE ELEMENT IN ESCHERICHIA COLI[J];Chinese Journal of Biotechnology;1990-04
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【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Yuwen Hao Hou Yunde Li Zhiliang Institute of Virology, Chinese Academy of Preventive Medicine, Beijing Department of Biology, Zhong Shan University, Guangzhou;USE OF ESCHERICHIA COLI PLASMID FOR DETECTION AND ISOLATION OF PROMOTERS FROM VACCINIA VIRUS GENOME[J];Chinese Journal of Virology;1987-03
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3 Gao Feng Liu Chongbai (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);STUDY ON PROTEINS SYNTHESIZED BY DIFFERENTSTRAINS OF HEPATITIS A VIRUS AT EARLY STAGEOF INFECTION IN CELL CULTURE[J];Chinese Journal of Virology;1989-02
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Chinese Journal Full-text Database 10 Hits
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