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《Chinese Journal of Virology》 1990-02
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PURIFICATION AND PHYSICO-CHEMICAL CHARACTERISTICS OF A GOSLIN PLAGUE VIRUS

Li Maoxiang Li Junbao Zheng Yumei Tianjin Poultry Disease Diagnosis Centre, Tianjin Jiangsu Agricultural College, Yangzhou, Jiangsu  
The procedures for the concentration and purification of the Goslni plague virus causing goslin hepatitis and hemorrhagic enteritis with a highly fatality were described. Some physico-chemical properties of the virus were studied.The viral exists in the major band of 1.31-1.35g/ml in CsCl and consists of full and empty particles 20-22nm in diameter with a sedimentation cceffecient of 90.5S in 15% Sucrose and 0.9% NaCl. The virus purified by Sepharose 4B column chromatography has a pI of 4.3 and contains three major structural polypeptides, Vp1, Vp2 and Vp3, with molecular weights of 85,000, 61,000 and 57,500 respectively.
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