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《Chinese Journal of Virology》 1991-01
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Zhou Weisong Liu Xiufan( Department of Animal Science and Veterinary Medicine, Jiangsu Agricultural College )  
A panel of 23 hybridoma cell lines were used to prepare McAb specific for Newcastle disease virus ( XDV ). Nine of them showed neutralizing activity. All the McAbs inhibiting hemolysis had neutralizing capability, indicating that virus infectivity is correlated with the ability of virus to induce cell fusion. Incubation of NDV with neutralizing McAbs resulted in various degree of neutralization. Neutralizing titers in plaque redaction neutralization, cytopathic effect ( CpE ) inhibition and chick embryo neutralization assays were found to be parallel. The infectious cell fluorescent focus formation units ( ICFFU ) was reduced up to 77.6% when virus adsorbed cells were incubated with L15-6 for lhr at 4℃. CPE was inhibited by 4 McAbs(10-1, 15-1, L15-6 and LA15-7) when they were added to NDV-infected Vero cells. Immunofluorescent assay showed that virus antigen still existed in the cytoplasm of CpE inhibited cells, and CpE re-emerged in 40hr after replacement with medium without McAbs. It was postulated that McAbs could neutralize NDV by inhibiting virus adsorbtion and penetration as well as by releasing adsorbed virus from cells. Virus present in the cells could not be completely neutralized by McAbs. McAbs 15-1 and L15-6 were capable of protecting Sp F chicks aganist F48-Es virulent strain challenge at a dose of 100 LD50. McAb 15-1 showed promising therapeutic effect ia chicks even after clinical signs of experi-mantal Newcastle disease ( ND ) had appeared.
【Fund】: 国家“七五”重点攻关项目
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1 Dong Wei Wen Qiyi Wu Yantao Zhang Rukuan Liu Xiufan (Dept. of Vet Med., Ani. Sci. and Vet. Med. Coll., Yangzhou Univ., Yangzhou 225009);IDENTIFICATION OF POLYPEPTIDES EPITOPE WITH NDV McAbs BE TO[J];JIANGSU AGRICUTURAL RESEARCH;1999-03
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1 Xu Xiulong Liu Xiufan(Department of Animal Science and Veterinary Medicine, Jiangsu Agricultural College);MONOCLONAL ANTIBODIES TO NEWCASTLE DISEASE VIRUS AND THEIR USE IN THE DETECT10N OF ANTIGENIC VARIATION[J];Chinese Journal of Virology;1988-01
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1 Xu Fuliang Zhang Rukuan Liu Xiufan (Animal Infections Disease Laboratory, Jiangsu Agric. Coll., Yangzhou 225009);THE CONDITIONS OF CHICKEN FLOCK SHEDDING VIRULENT NEWCASTLE DISEASE VIRUS AND EGG PRODUCTION IN AN INFECTED IMMUNE CHICKEN FLOCK[J];;1998-02
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1 Cheng Youquan Li Yiying Ling Tianlong Huang Nin Zheng Qi Song Tieying Wu Ping( Institute of Animal Husbandry and Veterinary Medicine, Fujian Acodemyof Agricultural Sciences );CHARACTERIZATION AND APPLICATION OF MONOCLONAL ANTIBODIES TO NEWCASTLE DISEASE VIRUS[J];Chinese Journal of Virology;1987-04
2 Xu Xiulong Liu Xiufan(Department of Animal Science and Veterinary Medicine, Jiangsu Agricultural College);MONOCLONAL ANTIBODIES TO NEWCASTLE DISEASE VIRUS AND THEIR USE IN THE DETECT10N OF ANTIGENIC VARIATION[J];Chinese Journal of Virology;1988-01
3 Chen Boquan Wu Meiying Ye Qunrui (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);COMPARATIVE STUDIES OF DIFFERENT PURIFICATION METHODS FOR MONOCLONAL ANTIBODY ( McAb )[J];Chinese Journal of Virology;1990-02
4 Luo Tingrong Yu Kelun Wei Jiahuai(Guangxi Agricultural College);DETECTION OF NEWCASTLE DISEASE VIRUS ( NDV ) BY ABC-ELISA[J];Chinese Journal of Virology;1990-03
5 Sun Yi Chen Boquan ( Institute of Virology, Chinete Academy of Preventive Medicine, Beijing );STUDY ON THE "EARLY DEATH" SYNDROME IN MICE BY USING MONOCLONAL ANTIBODIES TO RABIES VIRUS[J];Chinese Journal of Virology;1991-02
6 Cheng Youquan Li Yiying Wu PingLing Tianlong Zhuang Xiangsheng Huang NinFujian Academy of Agricultural Sciences, Fuzhou;CHARACTERIZATION AND APPLICATION OF MONOCLONAL ANTIBODIES( McAb )TO PSEUDORABIES VIRUS ( PrV )[J];Chinese Journal of Virology;1992-02
7 $$$$Wang Zhiliang;Peng Daxin;Zhang Rukuan;Jiao Xinan;Cui Hong; Hou YundeL Liu Xiufan (Department of Veterinary Science ,Yangzhou University,Yangzhou 225009)On the basis of cloned nonessential regions of fowlpox virus (FPW) DNA from Chinesevaccine stiain 2;CONSTRUCTION OF RECOMBINANT FOWLPOX VIRUS FROM CHINESE VACCINE STRAIN 282M AND EXPRESSION OF MAREKS DISEASE VIRUS gB GENE[J];Chinese Journal of Virology;1996-01
8 WU Yan tao,NI Xue xia,WAN Hong quan,LIU Wen bo,LIU Xiu fan (Laboratory of Animal Infectious Diseases,Yangzhou University,Yangzhou 225009,China);Molecular Epidemiological Characterization of Newcastle Disease Virus Isolates from China[J];Chinese Journal of Virology;2002-03
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【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 LI Jie-feng1,XIE Chun-fu1,CAO He2,SUN Ji-guo3,LIU Hong1,LI Qing-suo1(1.Hebei Provincial Husbandry and Veterinary Research Institute, Baoding, Hebei,071000, China;2. Tianjin Dairy Cattle Center, Tianjin, 300221, China;3.College of Animal Science and Technology, Hebei Agricultural University, Baoding, Hebei,071001, China);Cloning and Genetic Variation Analysis of the HN Gene of Newcastle disease virus Isolates[J];Progress in Veterinary Medicine;2009-03
2 JIANG Yan 1,ZHANG Chang yin 2,HUANG Wei jian 1,CHEN Pu yan 1 (1 Key Laboratory of Animal Diseases Diagnostic and Immunology,Ministry of Agriculture, Nanjing Agric Univ,Nanjing 210095,China;2 Jiangsu Entry Exit Inspection and Quarantine Bureau,Nanjing 210001,China);Construction of a pseudorabies virus deletion mutant containing GFP-Lac Z genes[J];Journal of Nanjing Agricultural University;2003-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Huijiao( Control Institute of Veterinary Bioproducts and Pharmaceuticals Ministry of Agriculture, Beijing );STUDIES ON BIOLOGICAL PROPERTIES OF ISOLATES OF NEWCASTLE DISEASE VIRUS IN CHINA[J];Chinese Journal of Virology;1985-04
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