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《Chinese Journal of Virology》 1992-02
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THE CONSTRUCTION AND BIOLOGICAL PROPERTIES OF A RECOMBINANT VACCINIA VIRUS(VMS11HAV25)EXPRESSING HEPATITIS A VIRUS ANTIGENS

Gao Feng Guo Kejian Ruan Li Yi YaoLiu Chongbo Zhu Jiming (C.M. Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing  
A HAV recombinant vaccinia virus named VMS11HAV25 was constructed by inserting the entire open reading frame of HAV cDNA,driven by a vaccinia promotor P11, into Hind 1 M fragment of Tian Tan strain of vaccinia virus which had been used extensively for over 50 years as a vaccine to eradicate smallpox in China.The inserted HAV cDNA was modified by deleting the majority of 5' noncoding region to prevent the production of infectious hepatitis A viral particles.It is important to know if the insertion of HAV gene affects the biological properties of the vector virus. Results showed that there were not much difference between the recombinant virus and the parental virus. An obvious difference was that the virulence of the recombinant virus was about ten times lower than its parental virus when tested in rabbits intradermally and in mice intracerebrally.The expression of HAV antigen by the recombinant virus at the 15th passage in human lung diploid cells was as good as that at the 4th passage. Southern blot hybridization showed that the inserted HAV cDNA was still located at the correct site after the recombinant virus had undergone 20 passages in the diploid cell culture, indicating the stability of the HAV gene in the vector.
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【References】
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【Citations】
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【Co-citations】
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1 FU Wei et al(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005);Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-Mouth Disease Virus(FMDV) Type O[J];Journal of Anhui Agricultural Sciences;2008-35
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10 Shang Xiangnian Jiang Qing Ma XiaoqianZheng Huifang Li Mingfa Mao Yiping Xie WeiWang Zhaoming Liu Jihe* Zhang Jianming(Nanjing Railway Medical College, Nanjing, 210009);DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMA ACUMINATA AND PAPILLOMA FROM EXTERNAL EAR CANAL USING POLYMERASE CHAIN REACTION[J];Chinese Journal of Virology;1993-04
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1 Pan Tie-li Zhang Shou-feng Hu Rong-liang(Military Veterinary Institute,Academy of Military Medical Sciences,PLA,Changchun 130062,China);Effect of Several Physical and Chemical Factors on Infectivity of Rabies virus[A];[C];2008
2 ;Restudy on effect of main physical and chemical factors on infectivity of a rabies virus isolate[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 Guo Kejian Gao Feng Yi Yao Liu Chongbo Wang Xiaojie Ruan Li Zhu Jiming ( C.M.Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;IMMUNOGENICITY OF A RECOMBINANT VACCINIA VIRUS (VMS11HAV25) EXPRESSING HEPATITS A VIRUS ANTIGENS[J];Chinese Journal of Virology;1992-01
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【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Ren Guifang Zhang Yiming Ruan Weiqin Zhu ChunZhu JimingDepartment of Viral Genetics, Institute of Virology, China National Centre for Preventive Menicine;STABILITY, KINETICS AND BEST CONDITIONS FOR HBsAg EXCRETION FROM B16 MOUSE CELL AND ITS CIONED SUBLINE CELLS[J];Chinese Journal of Virology;1985-01
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3 Tan Wei Li Hemin Hu Zonghan(National Institute for the Control of Pharmaceutical and BiologicalProducts , Beijing );CHARACTERISTIC OF PLAQUES OF RECOM-BINANT VACCINIA VIRUSES CONTAINING HBsAg GENE AT TK INSERTION SITE[J];Chinese Journal of Virology;1990-01
4 Gao Feng Yi Yao Zhao Honglan Liu Chongbo (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);INDUTION OF HEPATITIS A VIRUS NEUTRALZIING ANTIBODY IN RABBITS IMMUNIZED WITH RECOMBINANT VACCINIA VIRUS[J];Chinese Journal of Virology;1990-03
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