CHARACTERIZATION AND APPLICATION OF MONOCLONAL ANTIBODIES( McAb )TO PSEUDORABIES VIRUS ( PrV )
Cheng Youquan Li Yiying Wu PingLing Tianlong Zhuang Xiangsheng Huang NinFujian Academy of Agricultural Sciences, Fuzhou
Twenty three hybridoma cell liaes secreting McAbs against PrV have been established with 4 PrV strains as immunogens. On the basis of their immunobiological characters, the McAbs could be divided into 4 classes( 1 ) positive by FA, ELISA and Western blot, ( 2 ) positive by FA and ELISA; ( 3 ) positive by FA and Western blot; ( 4 ) positive by FA only. The results of experiments showed that five PrV strains could be classified into 2 different antigenic groups with the McAblO by ELISA and Western blot. Three methods, FA, reverse passive hemaggiutination(RPHA) and ELISA, have been used for detecting PrV antigen. And the results indicated that FA and RPHA are more sensitive;their concordance rates with virus isolation ( VI)were 88.2% and 76.5%, respectively. With the advantage of simplicity in performance, and no need of sophisticated equipment, RPHA is a suitable test for the diagnosis of pseudora bies in the field.