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《Chinese Journal of Virology》 1992-03
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GENERATION OF A MODIFIED INTERFERON-α2b GENE AND ITS EXPRESSION IN ESCHERICHIA COLII

Zeng Qing Jin Dongyan Jin Qi Hu Gang Zhou Yuan Li Yuying Wang Xunzhang Hou Yunde National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijng  
A modified human IFN-α2b gene was generated from that of IFN-α2a by substitution of the codon AAA for lysine as amino acid residue 23 with CGT for arginine via oligonucleotide-mediated site-directed mutage nesis. The resulting gene was characterized by digestion with restriction enzymes and the mutation was confirmed by DNA sequencing of the complete coding region. The IFN-α2b gene was expressed efficiently in E. coli with a vector pBV220 under the control of bacteriophage λpRpL tandem promoters and a comparison between the antiviral activities of several subtypes and sub-subtypes of IFN-α which are all abundantly synthesized in the pBV220-E.coli system has been carried out.
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