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《Chinese Journal of Virology》 1993-01
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EXPRESSION OF HUMAN TUMOR NECROSIS FACTOR-α BY VACCINIA VIRUS VECTOR IN ANIMAL CELLS AND A STUDY OF ITS PROPERTIES

Wan Xiaoyu Wu Shuhua Huang Liwen Zhang Qiuwang Zhang Lilan Hou Yunde( National Laboratory of Molecular Virology and Genetic Engineering, Institut, of Virology, Chinese Academy of Preventive Medicine, Beijing, 1 0052)  
Two recombinant vaccinia viruses named RVJTNF and RVJTNFPCR were constructed by inserting the modified and unmodified hTNF-α cDNA, driven by the promoter of 11kD protein of vaccinia virus, into the tk region of Tian Tan strain of vaccinia virus respectively. The modified hTNF-α cDNA was obtained by deleting the 5' and 3' noncoding reions using PCR technique. Southern blot hybridization showed that hTNF-α cDNA were inserted into the tk region of recombinant vaccinia virus correctly. Expression of TNF protein was assayed by cytotoxic activity for actinomycin D-treated L929 cell. Cultures infected by recombinant vaccinia virus secreted as muca as 1.6×104 units/ml of TNF of culture medium. Synthesis of TNF protein was confirmed by neutra-lizing cytotoxic activity of infected culture supernatant with a monoc-lonal antibody to TNF-α and by immunoprecipitation of 17kD protein from infected culture supernatant. Compared with the unmodified hTN F-α cDNA, the expression Ievel of modified hTNF-α cDNA increased 3 folds approximately. The antiviral activity of TNF-α expressed in mam-malian celis was higher than that of TNF-a expressed in E.coli.
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Chen, LIU Zhi-wei,GUO Yong (Department of Biology,Jiaying University, Meizhou 514015,China; College of Food Engineering and Biotechnology,South China University of Technology, Guangzhou 510640, China);Research Progress of Tumor Necrosis Factor and Its Genetic Engineering[J];Journal of Jiaying University;2002-06
2 Chen Hongyu, Ma Hongjin, Wei Shumin. (Beijing Institute for Cancer RESEARCH , Beijing 100034);Molecular cloning and expression of human CD28 gene in vaccinia virus system[J];Chinese Journal of Microbiology and Immunology;1996-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Tsao Hsu Liu Genqi Zhu Jiming(Chu Chiming) (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing );PROMOTER-LIKE FUNCTION OF VACCINIA VIRUS DNA FRAGMENTS IN E.COLI[J];Chinese Journal of Virology;1989-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FU Wei et al(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005);Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-Mouth Disease Virus(FMDV) Type O[J];Journal of Anhui Agricultural Sciences;2008-35
2 REN SHU-RONG,GAO MEI-HUA,WANG QIU-BO, et al(Department of Immunology,Qingdao University Medical College,Qingdao 266021,China);CONSTRUCTION AND TRANSFECTION OF HUMAN MUTANT CD59 EUKARYOTIC EXPRESSION SYSTEM[J];Acta Academiae Medicinae Qingdao Universitatis;2006-02
3 Ruan Li Zheng Haoqiang Xu Shuichan Wang Shuangping Tsao Xu Xie Yanxang Zhu Jiming (C. M. Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;EXPRESSION OF BOTH GLYCOPROTEIN F AND G OF RESPIRATORY SYNCYTIAL VIRUS ( RSV ) IN A SINGLE RECOMBINANT VACCINIA VIRUS[J];Chinese Journal of Virology;1992-02
4 Gao Feng Guo Kejian Ruan Li Yi YaoLiu Chongbo Zhu Jiming (C.M. Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;THE CONSTRUCTION AND BIOLOGICAL PROPERTIES OF A RECOMBINANT VACCINIA VIRUS(VMS11HAV25)EXPRESSING HEPATITIS A VIRUS ANTIGENS[J];Chinese Journal of Virology;1992-02
5 Zeng Qing Jin Dongyan Jin Qi Hu Gang Zhou Yuan Li Yuying Wang Xunzhang Hou Yunde National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijng;GENERATION OF A MODIFIED INTERFERON-α2b GENE AND ITS EXPRESSION IN ESCHERICHIA COLII[J];Chinese Journal of Virology;1992-03
6 Zhou Yuan Jin Dongyan Zhang Zhiqing Wang Wei Li Zhiliang Chi Jie Li Yuying Hou Yunde( National Laboratory of Molecular Biology and Genetic Engineering, Institute of Virology, Chtnese Academy of Preventive Medicine, Beijing 100052);PURIFICATION AND CHARACTERIZATION OF A NOVEL ENGINEERED MUTANT OF HUMAN α1-TYPE INTERFERON--IFN-α1/86D[J];Chinese Journal of Virology;1993-01
7 Wang Xunzhang Long Qingxin Xie Weidong Pang Yi Pu Zhelong( Instiiute of Entomology, Zhongshan University and National Loboratory of Biological Controt, Guangzhou,510275 );CHARACTERIZATION OF A BACULOVIRUS PROTEIN KINASE AND PHOSPHORYLATION AND DE-PHOSPHORYLATION OF THE VIRUS BASIC CORE PROTEIN[J];Chinese Journal of Virology;1993-01
8 Shang Xiangnian Jiang Qing Ma XiaoqianZheng Huifang Li Mingfa Mao Yiping Xie WeiWang Zhaoming Liu Jihe* Zhang Jianming(Nanjing Railway Medical College, Nanjing, 210009);DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMA ACUMINATA AND PAPILLOMA FROM EXTERNAL EAR CANAL USING POLYMERASE CHAIN REACTION[J];Chinese Journal of Virology;1993-04
9 CHEN Li, ZHANG Li bi, HOU Xiao hui, LI Jie (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China);Complete Nucleotide Sequence of New Enterovirus Isolated from the Cerebrospinal Fluid of Meningoencephalitis Patients[J];Chinese Journal of Virology;2000-04
10 DI Biao1,HE Li-juan1,ZHOU Duan-hua 1,GAO Yang1,WU Xin-wei1,YANG Wei-l u1,WANG Ming1,DU Lin1,LIU Yu-fei 1,QIU Ji-chun1,CHEN Xiao-shuang1,CHE N Fang1,ZHANG Wei1,LU En-jie1,YANG Huan-ming2,BI Sheng-li3(1.Guangzhou Center for Disease Co ntrol and Prevention,Guangzhog 51 0080,China;2.Human Genomic Center, Institute of Heredity,Chinese Acad emy of Sciences,Beijing 100101,Chi na;3.National Institute for Viral D isease Control and Prevention,Chin a CDC,Beijing 100052,China);Coronavirus the Main Cause for Epidemic of Severe Acute Respiratory Syndrome in Guangzhou Region[J];Chinese Journal of Virology;2003-03
China Proceedings of conference Full-text Database 2 Hits
1 Pan Tie-li Zhang Shou-feng Hu Rong-liang(Military Veterinary Institute,Academy of Military Medical Sciences,PLA,Changchun 130062,China);Effect of Several Physical and Chemical Factors on Infectivity of Rabies virus[A];[C];2008
2 ;Restudy on effect of main physical and chemical factors on infectivity of a rabies virus isolate[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Ruan Li Zheng Haoqiang Xu Shuichan Wang Shuangping Tsao Xu Xie Yanxang Zhu Jiming (C. M. Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;EXPRESSION OF BOTH GLYCOPROTEIN F AND G OF RESPIRATORY SYNCYTIAL VIRUS ( RSV ) IN A SINGLE RECOMBINANT VACCINIA VIRUS[J];Chinese Journal of Virology;1992-02
2 Wang Zhengfang; Zhang Qing; Lu Yong; Lu Haiyan (Second Institute of Oceanography, SOA, Hangzhou, 310012);The effects of Nutrients, Vitamins and Trace Metals on the growth of the red tide organism Prorocentmm micans[J];DONGAHI MARINE SCIENCE;1996-03
3 Zheng Suiping Guo Yong ( Bioengineering Institute, South-China University of Technology, Guangzhou 510641);Effects of staple nutrient on cell growth and anthocyanin production in suspension culture of Hibiscus sabdariffa L[J];GUIHAIA;1998-01
4 Chen Yifeng Zhou Xie (Department of Agronomy,Nanjing Agricultural University,Nanjing 210095);Effect of Choline Chloride on Membrane Stability of Crop Plants under Multiple Stresses[J];ACTA AGRICULTURAE BOREALL—SINICA;1997-02
5 WANG Chang-hai; OUYANG Fan(Inst. Marine Biochem. Eng., Yantai University, Yantai 264005, China)(State Key Lab. Biochem. Eng., Inst. Chem. Metall., CAS, Beijing 100080, China);The Cultures of Porphyridium cruentum in Photobioreactor[J];ENGINEERING CHEMISTRY & METALLURGY;2000-01
6 . Hu Qinhai et al.(Dept.of Environ.Sci.,Zhejiang Agricultural University,Hangzhou 310029);Effects of Rare-Earth Elements on Growth and Reproduction of Chlorella pyrenoides[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1996-02
7 Zhang Yiming Guo Siyuan (Light Industry and Chemical Eng. Reseach Institute, South China Univ. of Tech.);MIXOTROPHIC BATCH AND FED BATCH CULTURE OF SPIRULINA PLATENSIS[J];JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE);1999-09
8 Wang Jie Jiang Yuehua * Guo Yong Shi Dingji ** Zhang Hongbin * Liu Fenglong ** (Institue of Biotechnology, South China Univ. of Tech., Guangzhou 51064);CONSTRUCTION OF SHUTTLE VECTOR EXPRESSING THE RECOMBINANT HUMAN TUMOR NECROSIS FACTOR[J];JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE);1998-04
9 Qin Song; Yan Xiaojun; Zeng Chengkui (C. K. Tseng)(Institute of Oceanology, Chinese Academy of Science, Qingdao 2660071);TWO-YEAR REVIEW ON ALGAL MOLECULAR BIOTECHNOLOGY──MOLECULAR GENETICS AND GENETIC ENGINEERING[J];Oceanologia Et Limnologia Sinica;1996-01
10 Wu Jianqiu,Wang Xihua, Song Qin and C. K. Tseng (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071);STUDIES OF SELECTIVE MARKER FOR KELP TRANSFORMATION[J];Marine Sciences;1995-05
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Hu Yuwen Yuwen Hao Yang Xinke Hou YundeHu Gan Duan Shumin ( Institute of Virology, Chinese Academy of Preventive Medical Sciences );RESTRICTION ENDONUCLEASE SITE MUTATION WITHIN THE TERMINAL REGION OF GENOME OF VACCINIA VIRUS (TIAN TAN STRAIN)[J];Chinese Journal of Virology;1987-01
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