Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Virology》 1993-01
Add to Favorite Get Latest Update

PURIFICATION AND CHARACTERIZATION OF A NOVEL ENGINEERED MUTANT OF HUMAN α1-TYPE INTERFERON--IFN-α1/86D

Zhou Yuan Jin Dongyan Zhang Zhiqing Wang Wei Li Zhiliang Chi Jie Li Yuying Hou Yunde( National Laboratory of Molecular Biology and Genetic Engineering, Institute of Virology, Chtnese Academy of Preventive Medicine, Beijing 100052)  
The kinetics of the expression of a novel engineered mutant of hu-man α1-type interferon ( IFN-α1/86D)in E.coli grown in shaken flasks or fermentor was studied. The target protein was then purified to95% homogeneity through cation exchange chromatograpy ( CM-Sepharose ) and affinity chromatography ( monoclonal antibody against IFN-α1 ) , the purified protein with a specific activity of 2.3×107 IU/mg protein, appeared as a single band on the SDS-PAGE electrophoretogram and a single peak on the HPLC chromatogram. The N-terminal amino acid se-quence of purified recombinant human IFN-α1/86D was determined on Applied Biosystems 477A protein/peptide sequencer and was shown to be correct. The purified protein was demonstrated to be heterogenous, co mprising of two sequences which differ merely by the N-terminal Met residue-25% Met-plusspecies and 75% Met-minus.
【Fund】: 国家863高科技生物技术领域资助
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 5 Hits
1 Wu Aizhen\ Sun Yukun (Shanghai center of Biotechnology of Chinese Academy of Sciences,Shanghai\ 200233);Study on the Fermentation Technology of the Engineered Bacteria Escherichia coli[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1996-S1
2 Wu Jiajin\ Li Wuju\ Wang Jiaxi (Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing\ 100850);Analysis of the Factors of the Level of Expression of a Heterologous Gene in Prokaryocyte[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1996-S1
3 Yin Hongzhang et al (National Institute for the Control of Pharmaceutical and Biological Products, Beijing);Study on Murine McAbs to GM-CSF[J];Chinese Journal of Biologicals;1994-02
4 ZHANG Qing yi 1,2 , ZHANG Jing hai 1, HU Jin dong 2, HU Tao 2, CHEN Chang 2 (1. School of Pharmaceutical Engineering, Shenyang Pharmaceutial University, Shenyang 110016, China; 2. Shenyang Sunshine Pharmaceutical Co., Ltd., Shenyang 110027, China);Study on the isolation and purification pilot process of interferon α2a[J];Journal of Shenyang Pharmaceutical University;2003-04
5 RAN Xue-qin,WU Yong-jun,ZHU Wen-shi,WANG Jia-fu(College of Life Science,Guizhou University,Guiyang 550025,China);Expression of human interferon gamma-encoding gene in Esherichia coli[J];Southwest China Journal of Agricultural Sciences;2003-04
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Zhiqing Yao Lihong Hou Yunde(National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing );CONSTRUCTION AND APPLICATION OF A HIGH LEVEL EXPRESSION VECTOR CONTAINING P_RP_L PROMOTER[J];Chinese Journal of Virology;1990-02
2 Wang Wei Hou Yunde( National Laboratory of Molecular Virology and Genetic Engineering, Beijing);CONSTRUCTION OF AN INTERFERON MUTANT ( IFN-αⅠ/86D ) AND ITS BIOLOGICAL ACTIVITIES[J];Chinese Journal of Virology;1990-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Guang-cun1,YANG Yu1,WANG Xiu-li1,GAO Chang-yong1,CHEN Li-rong1,ZHANG Bin1,BI Yu-ping1,WANG Yi2(1Shandong Academy of Agricultural Sciences, Jinan 250100, China;2CIP Liaison Office in Beijing, Beijing 100081,China);Expression of potato virus Y coat protein gene in E.coli HB101[J];Shandong Agricultural Sciences;2003-01
2 FU Wei et al(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005);Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-Mouth Disease Virus(FMDV) Type O[J];Journal of Anhui Agricultural Sciences;2008-35
3 REN SHU-RONG,GAO MEI-HUA,WANG QIU-BO, et al(Department of Immunology,Qingdao University Medical College,Qingdao 266021,China);CONSTRUCTION AND TRANSFECTION OF HUMAN MUTANT CD59 EUKARYOTIC EXPRESSION SYSTEM[J];Acta Academiae Medicinae Qingdao Universitatis;2006-02
4 Zeng Weilan Lui Chongbai (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);HIGH LEVEL EXPRESSION OF HBV e ANTIGEN IN E.coli[J];Chinese Journal of Virology;1991-04
5 Gao Feng Guo Kejian Ruan Li Yi YaoLiu Chongbo Zhu Jiming (C.M. Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;THE CONSTRUCTION AND BIOLOGICAL PROPERTIES OF A RECOMBINANT VACCINIA VIRUS(VMS11HAV25)EXPRESSING HEPATITIS A VIRUS ANTIGENS[J];Chinese Journal of Virology;1992-02
6 Zeng Qing Jin Dongyan Jin Qi Hu Gang Zhou Yuan Li Yuying Wang Xunzhang Hou Yunde National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijng;GENERATION OF A MODIFIED INTERFERON-α2b GENE AND ITS EXPRESSION IN ESCHERICHIA COLII[J];Chinese Journal of Virology;1992-03
7 Wan Xiaoyu Wu Shuhua Huang Liwen Zhang Qiuwang Zhang Lilan Hou Yunde( National Laboratory of Molecular Virology and Genetic Engineering, Institut, of Virology, Chinese Academy of Preventive Medicine, Beijing, 1 0052);EXPRESSION OF HUMAN TUMOR NECROSIS FACTOR-α BY VACCINIA VIRUS VECTOR IN ANIMAL CELLS AND A STUDY OF ITS PROPERTIES[J];Chinese Journal of Virology;1993-01
8 Wang Xunzhang Long Qingxin Xie Weidong Pang Yi Pu Zhelong( Instiiute of Entomology, Zhongshan University and National Loboratory of Biological Controt, Guangzhou,510275 );CHARACTERIZATION OF A BACULOVIRUS PROTEIN KINASE AND PHOSPHORYLATION AND DE-PHOSPHORYLATION OF THE VIRUS BASIC CORE PROTEIN[J];Chinese Journal of Virology;1993-01
9 Zhang Zhiqing Zhang Ying Lu Xiuhua Wang Shicong Shao Kui Ma Lijuan Li Yuying Zhou Yuan Yao Lihong He Bingkun Hou YundeNational Laboratory of Molecular Virology and Genetic Engineering,Institute of Virology,Chinese Academy of Preventive Medicine, Beijing, 100052 Research Institute, North China Pharmaceutical Company, Shijiazhuong, 050015;INCREASED EXPRESSION OF HUMAN GM-CSF cDNA IN E.COLI BY 5' TERMINAL NUCLEOTIDE SEQUENCE MODIFICATION[J];Chinese Journal of Virology;1993-02
10 Shang Xiangnian Jiang Qing Ma XiaoqianZheng Huifang Li Mingfa Mao Yiping Xie WeiWang Zhaoming Liu Jihe* Zhang Jianming(Nanjing Railway Medical College, Nanjing, 210009);DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMA ACUMINATA AND PAPILLOMA FROM EXTERNAL EAR CANAL USING POLYMERASE CHAIN REACTION[J];Chinese Journal of Virology;1993-04
China Proceedings of conference Full-text Database 2 Hits
1 Pan Tie-li Zhang Shou-feng Hu Rong-liang(Military Veterinary Institute,Academy of Military Medical Sciences,PLA,Changchun 130062,China);Effect of Several Physical and Chemical Factors on Infectivity of Rabies virus[A];[C];2008
2 ;Restudy on effect of main physical and chemical factors on infectivity of a rabies virus isolate[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Cui Hong Hou Yunde(Institute of Virology, China National Centre for Preventive Medicine, Beijing);INFLUENCE OF HUMAN IFN-αD cDNA 3' NON-CODING SEQUENCE UPON GENE EXPRESSION IN E.COLI[J];Chinese Journal of Virology;1985-02
2 Jin Qi Yuwen Hao Hou Yunde (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);NUCLEOTIDE SEQUENCING OF A HUMANα-1 INTERFERON cDNA CLONE ESTABLISHED FROM NDV-F-INDUCED CORD BLOOD LEUCOCYTES[J];Chinese Journal of Virology;1987-03
3 Zhang Zhiqing Hou Yunte Li Yuying Zhao XiaoxiaZhou Yuan Duan Shumin Yao Lihong(Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);HIGH LEVEL EXPRESSION OF HUMAN INTERFERON-Y cDNA IN E.coli WITH A PLASMID CONTAINING PRPl PROMOTER AND Clts857 GENE[J];Chinese Journal of Virology;1988-02
4 Wu Shuhua Zhang Li Xing Zheng Hou Yunde Song Gan Zhang Lilan(Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);A COMPARATIVE STUDY ON THE ANTIVIRAL ACTIVITIES OF DIFFERENT TYPES OF HUMAN RECOMBINANT INTERFERONS[J];Chinese Journal of Virology;1988-03
5 Zhang Xiangming Tong Kuitang Liu Lan Hou Yunde National Institute for the Control of Pharmaceutical and BiologicalProducts.National Vaccine and Serum Institute, Shanghai.Institute of Virology, Chinese Academy of Preventive Medidne, Beijing.;A STUDY ON SOME BIOCHEMICAL FEATURES OF PURIFIED RECOMBINANT HUMAN INTERFERON ALPHA 1[J];Chinese Journal of Virology;1988-03
6 Zhang Dezhen Hou Yunde Wu Shuhua Su Chengzhi (Institute of Virology, Chinese Academy of Preventive Medicine,Beijing);PURIFICATION OF RECOMBINANT HUMAN INTERFERON GAMMA[J];Chinese Journal of Virology;1989-01
7 Li Yan Hou Yunde (Institute of Virology, Chinese Academy of Preventive Medicine,Beijing);AN ELISA FOR RECOMBINAT INTERFERON[J];Chinese Journal of Virology;1989-02
8 Wang Xiaoming Li Yuying Jin Qi Zhang Zhiqing Zhao Xiaoxia Hou Yunde(Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);CONSTRUCTION OF AN IFN-γ MUTANT (rlFN-γ 132-PGIL ) AND CHARACTERIZATION OF ITS ANTIVIRAL ACTIVITY[J];Chinese Journal of Virology;1989-04
9 Zhang Zhiqing Yao Lihong Hou Yunde(National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing );CONSTRUCTION AND APPLICATION OF A HIGH LEVEL EXPRESSION VECTOR CONTAINING P_RP_L PROMOTER[J];Chinese Journal of Virology;1990-02
10 Wang Wei Hou Yunde( National Laboratory of Molecular Virology and Genetic Engineering, Beijing);CONSTRUCTION OF AN INTERFERON MUTANT ( IFN-αⅠ/86D ) AND ITS BIOLOGICAL ACTIVITIES[J];Chinese Journal of Virology;1990-04
【Secondary References】
Chinese Journal Full-text Database 8 Hits
1 ZHANG Xue-xia CHEN Jing-chun (College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Molecular design and characterization of recombinant fusion protein of human IFNα_(2a)-THYα_1[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2006-01
2 WANG Rong 1,ZHAO Yingyi 2,ZHANG Yunkai 1,WEI Yutuo 1,LIN Ying 2 (1 Biotechnology Research Center,Guangxi Univ.,Nanning 530005,China; 2 College of Biotechnology and Sugarcane engineering,Guangxi Univ.,Nanning 530004,China);The study of fermentation conditions of genetic engineering microorganism[J];Journal of Guangxi Agricultured and Biological Science;2002-01
3 Liao Mei_de 1 Xie Qiu_ling 2 Lin Jian 2 Hong An 2 Sun Fen_yong 2 Liang Shi_zhong 1 (1. College of Food and Bioengineering, South China Univ. of Tech., Guangzhou 510640, China; 2. Institute of Bioengineering, Jinan Univ. Guangzhou 510632, Chi;Study on the Cultural Conditions of High Expression of Human Basic Fibroblast Growth Factor by Recombinant Escherichia coli hbFGF/BL21(DE3)pLysS[J];Journal of South China University of Technology(Natural Science);2002-06
4 DUAN Ming-hua1,PEI Jin1,XU Ren-hua2,JIAO Luo-ping2,JIN Lei2 (1.School of Pharmacy,Jilin University,Changchun 130012,China;2.Genescience Pharmaceuticals Co.,Ltd.,Changchun 130022,China);Preparation of recombinant human interferon α-2b solution[J];Journal of Shenyang Pharmaceutical University;2009-05
5 ZHOU Peng,ZHA Xiang-dong(School of Life Science Anhui University,Hefei 230039,China);Analysis of the level of expression of antimicrobial domains of granulysin in E.coli[J];Journal of Biology;2010-03
6 TAN Zhou-Jin~(1) XIE Bing-Yan~(2**) XIAO Qi-Ming~(3) YANG Yu-Hong~(2) FENG Lan-Xiang~(2)(Hunan Agricultural Universituy,Changsha 410128)~(1)(Institute of Vegetable and Flower,China Academy of Agricultural Sciences, Beijing 100081)~(2)(College of Biosafety Science and Technology,Hunan Agricultural Universituy,Changsha 410128)~(3);Studies on the Prokaryotic Expression Conditions of groEL from Endosymbionts in Bemisia tabaci[J];Microbiology;2005-04
7 LI Ying-Tao(Shanghai Hualian Pharmaceutical Co. Ltd., Shanghai 200083);Purification of Recombinant Human Interferon α-2b by Two-step Column Chromatographic Process[J];Chinese Journal of Pharmaceuticals;2004-03
8 BAI Jun jie, MA Jin, JIAN Qing, LI Xin hui, LOU Jian ren, LIN Wen hui, ZHANG Hong jun (Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Lab. of Tropical and Subtropical Fish Breeding and Cultivation Ministry of Agr;Study on modification of fish GH cDNA and its expression in Escherichia coli[J];JOURNAL OF FISHERY SCIENCES OF CHINA;2000-01
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Zhang Zhiqing Hou Yunte Li Yuying Zhao XiaoxiaZhou Yuan Duan Shumin Yao Lihong(Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);HIGH LEVEL EXPRESSION OF HUMAN INTERFERON-Y cDNA IN E.coli WITH A PLASMID CONTAINING PRPl PROMOTER AND Clts857 GENE[J];Chinese Journal of Virology;1988-02
2 Hou Yunde, Zhang Zhiqing, Yang Xinke, Mu Shuhua, Li Yuying, Peng Jinzhi, Li Yingxia, Yang Chongtai, Fu Qiulin, Hu Yuwen, Zhao Haiyan, Wang Lanzhi, Duan Shumin, Su Xiaoling, Zhang Xiuzheng, Zhang Lilan, Shi Lianyong and Liu Xiaorui(Department of Antivirals, Institute of Virology,Beijing);Cloning of Human a Interferon cDNA from Umbilical Cord Leucocytes and Its Expression in E. coli[J];Acta Academiae Medicinae Sinicae;1982-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved