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《Chinese Journal of Virology》 1993-01
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CHARACTERIZATION OF A BACULOVIRUS PROTEIN KINASE AND PHOSPHORYLATION AND DE-PHOSPHORYLATION OF THE VIRUS BASIC CORE PROTEIN

Wang Xunzhang Long Qingxin Xie Weidong Pang Yi Pu Zhelong( Instiiute of Entomology, Zhongshan University and National Loboratory of Biological Controt, Guangzhou,510275 )  
Cell-released and polyhedron-derived forms of Trichoplusia ni Nuclear Polyhedrosis Virus ( TnNPV)particles possess protein kinase activity and the activity was not enhanced by cyclic nucleotide. The Mg++ cation and pH optima for the protein kinase activity,which catalyzes the pho sphate bound to serine and threonine, was determined and found to be not particularly stringent. Phosphorylation of the virus basie core protein were also found in this study, regardless of whether the kinases used were from virus particles or infected cell extracts and whether phosphorylation was carried out in vitro or in vivo.The demonstration that phosphory1ation of the basie protein occured in most cases but not detected in purified [32p] orthophosphate-labelled virus suggests that de-phosphorylation of the protein takes place before virus packaging. The role for de-phosphorylation of the basie protein is discussed.
【Fund】: 高等学校博士学科点专项科研基金
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【Citations】
Chinese Journal Full-text Database 2 Hits
1 Wang Xunzhang Xie Weidong Long Qingxing Su Deming Zhong Jiang Pu Zhelong(Institute of Entomology, Zhongshan Univenity, Guangzhou) Virology Research Unit, Fudan University, Shanghai;A BACULOVIRUS EXPRESSION VECTOR SYSTEM WITH POLYHEDRA INCLUSION BODIES[J];Chinese Journal of Virology;1991-03
2 Wang Xun-zhang Xie Wei-dong He Dai-feng Zheng Xiao-wen Long Qing-xing Pang Yi Pu Zhe-long (Institute of Entomology, Zhongshan University, Guangzhou 510275);Characterization of Thymidine Kinase Activity Associated with the Replication of Trichoplusia ni Nuclear Polyhedrosis Virus[J];Virologica Sinica;1990-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 FU Wei et al(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005);Cloning and Prokaryotic Expression of VP1 Gene of Foot-and-Mouth Disease Virus(FMDV) Type O[J];Journal of Anhui Agricultural Sciences;2008-35
2 HE Jin et al(Guizhou Key Laboratory of Agricultural Bioengineering,Guiyang,Guizhou 550025);Isolation and Identification of One Strain Entomogenous Fungi and Preliminary Study on Its Insecticidal Activity[J];Journal of Anhui Agricultural Sciences;2009-10
3 Wang Yunxiang et al (State key laboratory of biological cortrol,Zhongshan University,Guanzhou 510275);Molecular cloning of spodoptera litura NPV(SlNPV) polyhedrosis gene[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1996-01
4 Liu Henlie Wu Zhentin Gan Changgao;STUDIES ON THE PATHOTOGICAL PHYSIOLOGY OF PIERIS RAPAE INFECTED BY BACILLUS THURINGIENSIS[J];Journal of Anhui Agricultural University;1985-02
5 Zhang Hangu (Tea Department of Anhui Agricutural College);PARASITES OF TEA PESTS AND THEIR REGULATION TO THE PESTS[J];Journal of Anhui Agricultural University;1989-02
6 LI Shi guang 1,LIN Hua feng 1,XU Qing feng 2,ZHANG Xiao mei 1 (1.Department of Plant Protection,Anhui Agricultural University,Hefei 230036; 2.College of Economics and Technology, China Science and Technology University);Studies on Pathogenicity of Several Fungal Species on Helicoverpa assulta[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-04
7 REN SHU-RONG,GAO MEI-HUA,WANG QIU-BO, et al(Department of Immunology,Qingdao University Medical College,Qingdao 266021,China);CONSTRUCTION AND TRANSFECTION OF HUMAN MUTANT CD59 EUKARYOTIC EXPRESSION SYSTEM[J];Acta Academiae Medicinae Qingdao Universitatis;2006-02
8 Gao Feng Guo Kejian Ruan Li Yi YaoLiu Chongbo Zhu Jiming (C.M. Chu )Institute of Virology, Chinese Academy of Preventive Medicine, Beijing;THE CONSTRUCTION AND BIOLOGICAL PROPERTIES OF A RECOMBINANT VACCINIA VIRUS(VMS11HAV25)EXPRESSING HEPATITIS A VIRUS ANTIGENS[J];Chinese Journal of Virology;1992-02
9 Zeng Qing Jin Dongyan Jin Qi Hu Gang Zhou Yuan Li Yuying Wang Xunzhang Hou Yunde National Laboratory of Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijng;GENERATION OF A MODIFIED INTERFERON-α2b GENE AND ITS EXPRESSION IN ESCHERICHIA COLII[J];Chinese Journal of Virology;1992-03
10 Wang Xunzhang Xie Weidong Long Qingxing Su Deming Zhong Jiang Pu Zhelong Institute of Entomology, Zhongshan University National Laboratory of Biological Control, Guangzhou;CONSTRUCTION OF BACULOVIRUS TRANSFER VECTOR PLASMIDS FOR DUAL GENE EXPRESSION[J];Chinese Journal of Virology;1992-03
China Proceedings of conference Full-text Database 2 Hits
1 Pan Tie-li Zhang Shou-feng Hu Rong-liang(Military Veterinary Institute,Academy of Military Medical Sciences,PLA,Changchun 130062,China);Effect of Several Physical and Chemical Factors on Infectivity of Rabies virus[A];[C];2008
2 ;Restudy on effect of main physical and chemical factors on infectivity of a rabies virus isolate[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Hu Yuwen Yuwen Hao Yang Xinke Hou YundeHu Gan Duan Shumin ( Institute of Virology, Chinese Academy of Preventive Medical Sciences );RESTRICTION ENDONUCLEASE SITE MUTATION WITHIN THE TERMINAL REGION OF GENOME OF VACCINIA VIRUS (TIAN TAN STRAIN)[J];Chinese Journal of Virology;1987-01
2 Pang Yi Xie Weidong Long Qingxing Chen Qijing Wang Xunzhang Pu Zhelong;Recombinant Baculovirus:Trichoplusia ni Nuclear Polyhedrosis Virus Containing a Fused Gene Encoding Escherichia coli β-Galactosidase[J];Acta Scifntiarum Naturalium Universitatis Sunyaatseni;1987-02
3 Wang Xunzhang;Xie Weidong;Long Qingxing Research Institute of Entomology;Recombinant Baculovirus——Ⅱ. Construction of a Baculovirus Transfer Vector Plasmid Carrying a Marker Gene[J];Acta Scifntiarum Naturalium Universitatis Sunyaatseni;1989-04
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