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《Chinese Journal of Virology》 1993-04
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Shang Xiangnian Jiang Qing Ma XiaoqianZheng Huifang Li Mingfa Mao Yiping Xie WeiWang Zhaoming Liu Jihe* Zhang Jianming(Nanjing Railway Medical College, Nanjing, 210009)  
Oligonucleotide primers of the E6 gene region of human papilloma-virus ( HPV)6,11 and 16 types were synthesized to detect HPV DNA in condyloma acuminata and papilloma using polymerase chain reaction ( PCR ) . The tissue samples were obtained from colposcopically directed biopsies and surgically excised specimens and specimens embeded in para-ffin. The 98 patients in this study consist of 78 condyloma acuminata, 16 papilloma in external ear canal and 4 pseudocondyloma. In female patients with genital condyloma acuminata, HPV DNA was positive for 4 ( HPV 6 ) , 12 ( HPV 11 ) and 49 ( HPV 6/11 ), respectively, in 66 cases. The total positive percentage was 98.4%. Positve HPV 6 DNA was foun-ded only in one case of 4 patients with pseudocondyloma.In male palien t, condyloma acuminata were obtained from penial and perianal region. Thse positive frequency of HPV DNA was 7/12 ( HPV 11) and 4/12 ( HPV 6/ 11 ). One patient was negative for HPV DNA. Therefore, the positive percentage of HPV DNA was 91.6%. Samples from 16 male patients with external ear canal were examined by HPV specific PCR amplification. Positive results for HPV 6/11 DNA, HPV 6/16 DNA, and HPV 6/11/16 DNA were obtained in five,three and eight cases respectively. Cases with typical koilocytes and without typical koilocytes in condyloma acuminata did not differ obviously in HPV DNA positive rates.
【Fund】: 江苏省卫生厅资助
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