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《Chinese Journal of Virology》 1993-04
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DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN CONDYLOMA ACUMINATA AND PAPILLOMA FROM EXTERNAL EAR CANAL USING POLYMERASE CHAIN REACTION

Shang Xiangnian Jiang Qing Ma XiaoqianZheng Huifang Li Mingfa Mao Yiping Xie WeiWang Zhaoming Liu Jihe* Zhang Jianming(Nanjing Railway Medical College, Nanjing, 210009)  
Oligonucleotide primers of the E6 gene region of human papilloma-virus ( HPV)6,11 and 16 types were synthesized to detect HPV DNA in condyloma acuminata and papilloma using polymerase chain reaction ( PCR ) . The tissue samples were obtained from colposcopically directed biopsies and surgically excised specimens and specimens embeded in para-ffin. The 98 patients in this study consist of 78 condyloma acuminata, 16 papilloma in external ear canal and 4 pseudocondyloma. In female patients with genital condyloma acuminata, HPV DNA was positive for 4 ( HPV 6 ) , 12 ( HPV 11 ) and 49 ( HPV 6/11 ), respectively, in 66 cases. The total positive percentage was 98.4%. Positve HPV 6 DNA was foun-ded only in one case of 4 patients with pseudocondyloma.In male palien t, condyloma acuminata were obtained from penial and perianal region. Thse positive frequency of HPV DNA was 7/12 ( HPV 11) and 4/12 ( HPV 6/ 11 ). One patient was negative for HPV DNA. Therefore, the positive percentage of HPV DNA was 91.6%. Samples from 16 male patients with external ear canal were examined by HPV specific PCR amplification. Positive results for HPV 6/11 DNA, HPV 6/16 DNA, and HPV 6/11/16 DNA were obtained in five,three and eight cases respectively. Cases with typical koilocytes and without typical koilocytes in condyloma acuminata did not differ obviously in HPV DNA positive rates.
【Fund】: 江苏省卫生厅资助
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