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《Chinese Journal of Virology》 2001-03
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Study on Malignant Transformation of Human Embryonic Liver Cells Induced by Hepatitis B Virus and Aflatoxin in Nude Mice

LAN Xiang-ying 1 GUO Xiu-chan 1 ZHOU Ling 1 ZHANG Yong-li 1 SHEN Zhong-ying 2 ZENG Yi 1 (1 Institute of Virology,CAPM,Beijing 100052,China;2 Medical College of Shantou University,Shantou 511031,China)  
In order to study the effect of HBV and aflatoxin in hepatocarcinogenesis,the human embryonic liver cells infected with HBV were transplanted into nude mice by subcutaneous injection The transplanted nude mice were divided into 4 groups Group A(HBV+AFB 1):nude mice were injected with HBV infected human embryonic liver cells,then injected with AFB 1 once a week.Group B(HBV +):nude mice were treated with HBV as Group A ,but no AFB 1 was injected. Group C(AFB + 1):nude mice were injected with normal human embryonic liver cells and treated with AFB 1 as Group A Group D:control group,nude mice were injected with normal human embryonic liver cells and no AFB 1 was used Results:The incidence of tumor in different group was,group A 27 3%(6/22),group B 0% (0/13),group C 13 3%(2/15)and group D 0% (0/14) All the tumors were proved to be human hepatocellular carcinomas(HCCs)by pathological diagnosis The tumor tissues were anthropogenetic tested by EMA monoclonal antibody The HBV-X and HBV-S genes were positive by slot hybridization and PCR amplification,indicating that HBV DNA genes had integrated into cellular DNA We have first successfully induced human HCC with HBV and AFB 1 in nude mice This study demonstrated that there is a synergetic effect between HBV and AFB 1 in human hepatocarcinogenesis and will offer an animal model for further research on liver cancer
【Fund】: 国家自然科学基金重点项目 (3 983 0 3 80 )
【CateGory Index】: R730.2
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【References】
Chinese Journal Full-text Database 1 Hits
1 GUO Xiu-chan,LAN Xiang-ying,ZHOU Ling,TENG Zhi-ping, ZHANG Yong-li,CHEN Jiong-yu,SHEN Zhong-ying,ZENG Yi (1 Institute of Virology,CAPM,Beijing 100052,China;2 Molecular Biology Lab ,Institute of Hematology, Beijing University,Beijing;Establishment of Human Hepatocellular Carcinoma Cell Line Induced by Synergetic Effect of Hepatitis B Virus and Aflatoxin[J];病毒学报;2001-03
【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZhouYuanping,PengWenwei,YaoJilu,TheThirdHospitalAffiliatedtoSunYat-senUniversityofMedicalSciences,Guangzhou510630;SynergismofThreeRiskFactorsinHepatocelularCarcinogenicity[J];中华预防医学杂志;1996-05
2 Liu Zhensheng; Li Baomin; Liu Yanfang; Zeng Yi(Institute of Virology, CAPM, Beijing, 100052) Twenty Two Balb/c nude mice were transplanted subcutancously with human fetalnasopharyngeal tissure infected with B95-8 EB vinis. Butyric acid and/or 12- 0-tetrad;STUDIES ON HUMAN NASOPHARYNGEAL MALIGNANTLYMPHOMA AND UNDIFFERENTIATED CARCINOMAINDUCED BY THE SYNERGETIC EFFECT OF EB VIRUSAND TUMOR PROMOTER[J];病毒学报;1996-01
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 YUAN Hui , YANG Bei bei, XU Ze feng Department of Otorhino laryngology, Second Affiliated Hospital of Medical School, Zhejiang University, Hangzhou 310009, China;The clinical value of quantitative analysis of plasma Epstein-Barr virus DNA in patients with nasopharyngeal carcinoma[J];中华耳鼻咽喉科杂志;2004-03
2 GUO Xiuchan ,SHENG Wang,ZHANG Yongli,et al. Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China;Malignant transformation of monkey kidney epithelial cell induced by EBV BARF1 gene and TPA[J];中华实验和临床病毒学杂志;2001-04
3 HUANG Yan-ping 2,GUO Xiu-chan 1,HE Zu-gen 2,ZHAO Jian 1, ZENG Yi 1 (1.Institute of Virology, CAPM, Beijing 100052, China; 2.Tumor Hospital, CAMS and PUMC, Beijing 100021, China);Epstein-Barr Virus Induced Thymus Malignant T Cell Lymphoma[J];病毒学报;2001-04
4 LAN Xiang-ying 1 GUO Xiu-chan 1 ZHOU Ling 1 ZHANG Yong-li 1 SHEN Zhong-ying 2 ZENG Yi 1 (1 Institute of Virology,CAPM,Beijing 100052,China;2 Medical College of Shantou University,Shantou 511031,China);Study on Malignant Transformation of Human Embryonic Liver Cells Induced by Hepatitis B Virus and Aflatoxin in Nude Mice[J];病毒学报;2001-03
5 SHEN Zhongying(Email:jshen@mailserv.stu.edu.cn) *, CHEN Minghua, CAI Weijia, SHEN Jian, CHEN Jiongyu, HONG Chaoqun, Zeng Yi *Department of Pathology, Shantou University Medical College, Shantou 515031, Guangdong Province,China;The effects of sodium butyrate on proliferation, differentiation and apoptosis in immortalized esophageal epithelial cells[J];中华病理学杂志;2001-02
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 LAN Xiang-ying 1 GUO Xiu-chan 1 ZHOU Ling 1 ZHANG Yong-li 1 SHEN Zhong-ying 2 ZENG Yi 1 (1 Institute of Virology,CAPM,Beijing 100052,China;2 Medical College of Shantou University,Shantou 511031,China);Study on Malignant Transformation of Human Embryonic Liver Cells Induced by Hepatitis B Virus and Aflatoxin in Nude Mice[J];病毒学报;2001-03
2 Lu Peixin; Zhang Qinan; Wang Jinbing; et al(Qidong Liver Cancer Institute,Qidong 226200);The Relation between Hepatitis B Virus Infection or Aflatoxin Exposure and the Incidence of Primary Liver Cancer[J];中国肿瘤;1999-07
3 Teng Zhiping Zeng Yi(Institute of Virolog,Beijing,100052);DETECTION OF LMP GENE OF EPSTEIN-BARR VIRUSIN NASOPHARYNGEAL CARCINOMA BY IN SITUHYBRIDIZATION WITH BIOTIN LABELLED PROBES[J];病毒学报;1994-02
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zeng Yi Liu Yuxi Wei Jineng Zhu Jisong Cai Shaolin Wang Peizhong Zhong Jianming Li Ruicheng Pan Wenjun Li Erjie Tan Bifan Institute of Virology, Chinese Academy of Medical Sciences. Cancer Institute, Chinese Academy of Medical Sciences. People Hospital of Guangxi Zhuang Autonomous Region. Cancer Institute of Cangwu County.;SEROLOGICAL MASS SURVEY OF NASOPHARYNGEAL CARCINOMA[J];中国医学科学院学报;1979-02
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