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《Chinese Journal of Virology》 2002-02
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A Preliminary Studies on Relationship Between Gene Variation and Function of NSP4 of Human Rotaviruses

XU Shu shen,WANG Jian wei,HE Ya qing,JIANG Hui ying,HONG Tao (Institute of Viral Disease Control and Prevention,China CDC,Beijing 100052,China)  
A comparative study was carried out between the NSP4 cDNA derived from the rotavirus strains isolated from infants with light or heavy clinical symptoms.We found that there were remarkable variations in the presumed "virulent"region of aa 131-146 as reported in the literatures by others.Two NSP4 (from"light symptom"strain B53 and "heavy symptom"strain SZ1) were endogenously expressed in recombinant baculovirus infected Sf9 cells to see whether there was any influence upon intracellular metabolism of calcium by using confocal microscopy.The results demonstrated a parallel calcium increase with 3.1 to 3.4 times at 48h,and 5.6 to 5.8 times at 96h post-infection, but without noticeable difference.It is worthwhile to notify the fact that the above original observation had identified the calcium increasing ability of the NSP4 of the human rotavirus. What is more,the variation we observed in the "heavy symptom"strain SZ1 seemed not to elevate its virulence. The increased virulence of the heavy symptom strain seemed possibly irrelevant to variation of NSP4.
【Fund】: 国家自然科学基金资助项目 (编号 :3 0 0 0 0 14 5 ) ;; 国家863计划生物和现代农业技术领域资助项目 (编号 :2 0 0 1AA2 15 0 11)
【CateGory Index】: R373
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