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《Chinese Journal of Virology》 2003-02
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Expression of Capsid Protein VP1 of Enterovirus Type 71 in Pichia pastoris

YANG Guowei1,HE Yaqing2,XUE Ying1,ZHOU Shili1,MA Li1,JIN Qi1lecular Virology and Genetic Engineering,Beijing 100052,China;2.Shenzhen Epidemic Prevention Station,Shenzhen 518001,China)  
The gene encoding the entire capsid protein VP1 of enterovirus type 71(EV71) was amplified by RTPCR.After confirmed by sequence analysis,VP1 was expressed in Pichia pastoris (GS115).SDSPAGE showed that the geneVP1 could express product of 34kD as same as the natural protein.The density scanning of the SDSPAGE gel revealed that the target protein accounted for 65% of the total protein in the supernatant.ELISA analysis of the culture supernatant confirmed it had the desired immunogenicity of EV71.After concentrated by ammonium sulfate precipitation,the recombinant protein VP1 could specifically react with EV71 patients' sera,but not with antiCox A16 serum.By testing 156 sera with recombinant protein VP1 as antigen,it revealed that recombinant VP1 of EV71 may be an effective mean for determining EV71infection of different disease phases.
【Fund】: 国家"863"计划资助
【CateGory Index】: R373.3
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