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《Chinese Journal of Virology》 2003-02
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Cloning of Spike Protein S1 Gene of Avian Infectious Bronchitis Virus H Strain and Its Expression in Pichia pastoris

HUANG Ya-dong1,ZHENG Qing1,LI Xiao-kun1,WANG Lin-chuan2,ZHAO Zheng-fen3,HUANG Zi-ran4(1.Biopharmaceutic R&D Center of Jinan University,Guangzhou 510632,China;2.Department of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;3.College of Zhengzhou Animal Husbandry,Zhengzhou 450008,China;4.Department of Sericulture,South China Agricultural University,Guangzhou 510642,China)  
Avian ?infectious ?bronchitis ?virus (IBV) H ?strain ?isolated ? from ? Guangdong ?Province ?was ?grown ?in SPF chicken embryos and purified by differential centrifugation and its RNA was extracted by SDS-proteinase K method.According to the spike glycoprotein S1 gene sequence of IBV Beaudette strain,a pair of primers were designed and synthesized.With the primers,IBV H S1 gene was successively amplified by RT-PCR.The PCR product was digested wi簦? Bst Y Ⅰ,Hae  Ⅲ and  Pst  Ⅰ separately.The result showed that the RFLP pattern of IBV H S1 gene was the same as that of M41 S1 gene.IBV H strain was considered as Mass serotype.IBV H S1 gene was cloned into pGEM-T vector and sequenced,the result showed it consisted of 1611 base pairs(from ATG to the possible cleavage site of spike glycoprotein).By comparison,the nucleotide sequence of IBV H S1 gene was 97.39% and 97.27% identical to those of IBV M41 and Beaudette respectively.IBV H S1 gene was subcloned into expression vector pPICZ-A,SDS-PAGE experiment showed it was expressed in  Pichia pastoris.The expressed product had antigen specificity by determination of Western blot with avian anti-IBV serum.
【Fund】: 广东省青年基金项目(950416);; 国家自然科学基金项目(39470538)
【CateGory Index】: S852.65
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【References】
Chinese Journal Full-text Database 3 Hits
1 TIAN Zhan-cheng 1 ,WANG Yun-feng 2 ,TONG Guang-zhi 2 ,LIU Guang-yuan 1 (1.Lanzhou Veterinary Research Institute ,CAAS, Lanzhou,Gansu,730046,China; 2.Harbin Veterinary Research Institute,CAAS,Harbin,Heilongjiang,150001,China);The Development of Infectious bronchitis virus Vaccine[J];Progress In Veterinary Medicine;2005-06
2 XIE Hong-mei 1, ZHANG Xing-xiao2, ZHU Lai-hua 3, CHANG Wei-shan1** (1.College of Animal Science and Technology,Shangdong Agricultural University, Tai'an 271018, China; 2. Shandong Binzhou PBLV Biological Technology Research Institute,Binzhou 256600, China; 3. Qingdao Entry Exit Inspection and Quarantine Bureau, Qingdao 266002, China);Expression of S1 Gene of Infectious bronchitis virus in Escherichia coli and the Preliminary Identification of Its Antigen[J];Journal of Agricultural Biotechnology;2007-01
3 TIAN Zhan-cheng1,2,SUN Yong-ke1,WANG Yun-feng1,ZHI Hai-Dong1,LIU Sheng-Wang1,WANG Mei1,TONG Guang-Zhi1*(1.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Inner Mongolia University of Agriculture,Huhhot 010018,China);Construction of recombinant fowlpox virus expressing S1 gene of infectious bronchitis virus[J];Chinese Journal of Preventive Veterinary Medicine;2006-04
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Linchuan; Liu Fuan; Lin weiqing;Xin Chaoan(Animal Medicine Department, South China Agricultural University,Guangzhou 510642,China);Polymerase Chain Reaction Amplification of the S1 Glycoprotein Gene of Avian Infectious Bronchitis Virus[J];CHINESE JOURNAL OF VETERINARY MEDICINE;1994-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YU Miao(College of Life Science,Henan Normal University,Xinxiang,Henan 453007);Research Progress in the Effect of Crowding Stress on Fish[J];Journal of Anhui Agricultural Sciences;2008-03
2 CHEN Yan(College of Life Science and Technology,Yancheng Teachers College,Yancheng,Jiangsu 224002);Effects of Different pH values on the DNA Extraction Rate[J];Journal of Anhui Agricultural Sciences;2008-05
3 Wang Linchuan 1 Liao Min 1 Wang Hongnin 2 Huang Yong 2 Xin Chaoan 1;DIFFERENTIATION OF AVIAN INFECTIOUS BRONCHITIS VIRUS ISOLATED IN CHINA USING POLYMERASE CHAIN REACTION AND RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS[J];CHINESE JOURNAL OF VIROLOGY;1997-04
4 SHEN Wei-feng, NIU Bao-long, WENG Hong-biao, MENG Zhi-qi (Sericultural Research Institute, Zhejiang Academy of Agricultural Science,Hangzhou 310021, China);Cloning 6-phosphogluconate Dehydrogenase(6PGDH)of the Silkworm Bombyx mori by Selecting EST Database[J];Bulletin of Sericulture;2005-01
5 LI Shu-hua~1, HE Jun~1, LIU Wen-juan~2, YANG Shu-qin~2, HAN Guo-min~3 (1.Key Laboratory of Agricultural Biotechnological in Ningxia, Yinchuan 750002, China; 2.Ningxia University, Yinchuan 750021, China; 3.Crop Research Institute, Ningxia Academy of Agricultural and Forestry Sciences,Yongning 750105, China);Method of DNA extracted from O.rufipogon, Avena fatua L. and Lycium barbarum L.[J];Agricultural Research in the Arid Areas;2005-03
6 GANG Jie 1,LIU Shou-yi 2,ZHAI Li-ping 1,JIANG Yang 1,WU Yun-hong 1,JIANG Ji-feng 1 (1.Department of Food Engineering,Qiqihar University, Qiqihar 161006,China;2.Chifeng of Nei Monggol Youngling Teachers School,Chifeng 024000,China);Applying the teaching method of the net to biochemistry teaching[J];Journal of Science of Teachers College and University;2002-04
7 MO Meilan,WEI Ping,LI Kangran (Animal Science and technology College, Guangxi University, Nanning 530005);Advances in research on the diagnosis and geno typing of avian infectious bronchitis virus by reverse transcriptase polymerase chain reaction and restriction frgament length polymorphism analysis[J];JOURNAL OF GUANGXI AGRICULTURED AND BIOLOGICAL SCIENCE;2000-03
8 HONG Lei, ZHANG Xiu-mei (Fishery College, Ocean University of China, Qingdao 266003, China);Effects of Environmental Stress on Physiological Function of Fish[J];Advances in Marine Science;2004-01
9 ZHAO Cang-bi, HUANG Yu-dong, LI Yan-hui(Dept. of Applied Chemistry, Harbin Institute of Technology, Harbin 150001,China, );Extraction of collagen from cattle tendons[J];Journal of Harbin Institute of Technology;2004-04
10 REN Shu-ping(Huinan Center Middle Sclool, Wuhe Anhui 233322, China);Research Progression of Glycoprotein and Disease[J];Journal of Hefei University;2004-04
China Proceedings of conference Full-text Database 1 Hits
1 ZHAO Qi-bo,KONG Qing-ping, Alifu·aierxi, NING Xin-ming, LIU Zhi-qing)(Economic Crops of Xinjiang Academy of Agricultural Sciences Cotton Breeding Team 83000,China);Study about the Relation between Ensure System of Zyme SOD,POD Activity Changing and G.barbadense L.disease resistance and It’s mechanism[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Le-yi et al(Department of Animal Science,College of Agriculture,Liaocheng University,Liaocheng,Shandong 252059);Application of the ELISA Kit Made in China and USA for Detecting Antibody of Infectious Bronchitis[J];Journal of Anhui Agricultural Sciences;2005-09
2 CHENG Tai-ping et al(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025);Isolation and Identification of Nephropathogenic Strain of Avian Infectious Bronchitis Virus[J];Journal of Anhui Agricultural Sciences;2006-23
3 Jiang Guotuo Liu Siguo Bu Zhigao Kang Lijuan Lu Jingliang (State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001);EFFECT OF SITE MUTATION IN SPIKE 1 PROTEIN GENE ON IMMUNOGENICITY OF THE AVIAN INFECTIOUS BRONCHITIS VIRUS[J];CHINESE JOURNAL OF VIROLOGY;1999-03
4 ZHOU Ji-yong,ZHANG De-yong,DING Hong-mei,CHENG Li-qin,CHEN Ji-gang (Institute of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China);Genetic Variance Analysis of Nucleocapsid Protein Gene of Infectious Bronchitis Viruses with Different Tissue Tropism[J];Chinese Journal of Virology;2003-01
5 DAI Ya-bin 1,2,CHEN De-sheng 1,DING Chan 2,PAN Jie-yan 1,LIU Xing-you 1,LU Yin-hua 1, LIU Mei 2,CHEN Pu-yan 1,CAI Bao-xiang 1 (1. KeyLaboratory of Animal Disease Diagnosis and Immunology,Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095,China;2 Jiangsu Institute of Poultry Science,Yangzhou 225003,China);Construction of Recombinant Baculovirus Expressing S1 Glycoprotein of Infectious Bronchitis Virus Isolate JS/95/03[J];Chinese Journal of Virology;2003-01
6 CHEN De-sheng,PAN Jie-yan,CAO Rui-bing,DAI Ya-bin,CHEN Pu-yandiagnosis and Immunology,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China);Study on Molecular Epidemiology of Avian Infectious Bronchitis Viruses Isolated in China[J];Chinese Journal of Virology;2003-02
7 DU En-qi~2,LIU Sheng-wang~1,KONG Xia n-gang~1,TONG Guang-zhi~1,YANG Zeng- qi~2in Veterinary Medicine,Chinese Aca demy of Agricultural Sciences,Heilongjiang,Haerbin 150001,China;2.Col lege of Animal Science and Technolo gy,Northwest Sci-Tech University o f Agriculture and Forestry, Shaanxi, Yanglin g 712100,China);Expression of Avian Infectious Bron chitis Virus Nucleocapsid Protein Gene in E.coliand the Study of It s Antigenicity[J];Chinese Journal of Virology;2003-04
8 LIU Sheng wang 1,WANG Wei 2,LIU Yu fen 3,KONG Xian gang 1,TONG Guang zhi 1 (1.National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin,Heilongjiang 150001 China;2.College of Veterinary Medicine,Xinjiang Agricultural University,Xinjiang, Ulumuqi 83000, China;3.College of Veterinary Medicine,North east Agricultural University,Harbin,Heilongjiang 150030, China);Molecular Characteristics of Spike Gene of Infectious Bronchitis Virus LX4 Isolated in China[J];Chinese Journal of Virology;2004-01
9 ZHANG Qing-xia,CHEN Jian-fei,HAN Zong-xi,ZHOU Yu-chang,SHAO Yu-hao,RONG Jun-gong,LIU Sheng-wang,KONG Xian-gang(National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,Heilongjiang,China);Genetic Variations of Nucleocapsid Gene of Infectious Bronchitis Virus Strains Isolated in China during 1995-1999[J];Chinese Journal of Virology;2006-03
10 JIAO Hong-mei1,JIAO Xin-an1,2*,TIAN Hua-rong1,PAN Zhi-ming1,YIN Yue-lan1,CUI Yi-chen1,HUANG Jin-lin1 (1.Jiangsu Key Laboratory of Zoonosis;2.Animal Infectious Disease Laboratory of Ministry of Agriculture,Yangzhou University,Yangzhou 225009,China);DNA Vaccine of Infectious Bronchitis Virus s1 Gene Delivered by Attenuated Salmonella Typhimurium and Its Protective Efficacy[J];Chinese Journal of Virology;2007-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 JIAO Hong-mei1,JIAO Xin-an1,2*,TIAN Hua-rong1,PAN Zhi-ming1,YIN Yue-lan1,CUI Yi-chen1,HUANG Jin-lin1 (1.Jiangsu Key Laboratory of Zoonosis;2.Animal Infectious Disease Laboratory of Ministry of Agriculture,Yangzhou University,Yangzhou 225009,China);DNA Vaccine of Infectious Bronchitis Virus s1 Gene Delivered by Attenuated Salmonella Typhimurium and Its Protective Efficacy[J];Chinese Journal of Virology;2007-03
2 ZHANG Jian-chen (Shandong Vocational Animal Science and Veterinary College,Weifang 261061,China);Isolation of Avian Infectious Bronchitis Nephritis Virus and Cloning and Sequencing of the Virus N gene[J];Poultry Science;2008-09
3 ZHANG Qiu-wei,LIU Dong,CHEN Shu-ming(College of Animal Science and Technology,Shanxi Agricultural University,Taigu,Shanxi,030801,China);Advance in Prevention and Cure Technology of the Nephropathogenic infectious bronchitis in Chicken[J];Progress in Veterinary Medicine;2008-01
4 FAN Chun-bo1,2,WU Jian-hua2,AI Xiao-jie 1(1.Shanghai Jiaotong University Agricultural and Biological Technology,Shanghai,200240,China2.Fengxian District Animal Health Supervision,Fengxian,Shanghai,201400,China);Progress on IBV Immune Mechanisms and Immune Prevention and Control[J];Progress in Veterinary Medicine;2008-09
5 SHI Xing-ming,WANG Yun-feng,WANG Mei,LIU Sheng-wang,TONG Guang-zhi,XU Ling-long,HE sun (Division of Avian Infectious Diseases,National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China);Protection immunization of avian infectious bronchitis vaccine expressed by recombinant fowlpox virus after challenged with different IBV LHB strain[J];Journal of Guangxi Agricultural and Biological Science;2006-S1
6 Wang Changyuan1,Zhou Qifei2 ,Xue jun3,Wang Jichang4,Wen Xiaobo1(1.Heilongjiang August First Land Reclamation University,Daqing 163319;2.Heilongjiang Agricultural Engineering Professional Department;3.Guangming Veterinary Medicine Factory,JiNan;4.8510 Farm Veterinary Hospital of Heilongjiang Province);Clone and sequence analysis of N Gene of an Avian Infectious Bronchitis Virus[J];Journal of Heilongjiang August First Land Reclamation University;2008-03
7 JIAO Hong-mei,JIAO Xin-an,YIN Yue-lan,HUANG Jin-lin,PAN Zhi-ming,SUN Lin,ZENG Xian-ying,GU Zhi-qiang(Lab of Zoonosis and Immunol,Yangzhou Univ,Yangzhou,225009,China);Eukaryotic expression of S1 gene from infectious bronchitis virus and immunogenicity of its product[J];Journal of Yandzhou University;2006-02
8 WANG Yun-feng1,2,SHI Xing-ming2,WANG Mei2,LIU Sheng-wang2,TONG Guang-zhi2*,CUI Hong-yu2,MU Gu-li2,XU Ling-long2,HE Lai2,HE Sun2,GE Jun-wei1,LI Yi-jing1 (1.College of Veterinary Medicine,Northeast Agricultural University, Harbin 150030,China;2.Division of Avian Infectious Diseases,National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China);Partial Biological Character Detection of Fowlpox Virus Recombinant Co-expressing the IBV-S1 Gene and the Chicken IFNγ Gene[J];Chinese Journal of Animal and Veterinary Sciences;2007-10
9 SHI Xing-ming1,WANG Yun-feng1,WANG Mei1,LAN De-song1,REN Gang3,LI Ji-song1,ZENG Wei-wei1,SUN Yan1,LIU Sheng-wang1,TONG Guang-zhi2(1.Division of Avian Infectious Diseases,National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Shanghai Institute of Animal Parasitology,Chinese Academy of Agricultural Sciences,Shanghai 200232,China;3.Dongguan Extry Exit Inspection and Quarantine,Dongguang 523071,China);Safety Evaluation and Minimum Immune-doses Analysis of the Recombinant Fowl Pox Virus Expressing S1 Gene of Infectious Bronchitis Virus and IFN-γ Gene[J];Chinese Journal of Animal and Veterinary Sciences;2009-05
10 LI Chun - ming, PENG Yuan - yi (. College of Resources and Environment, Southwest University, Chongqing 400716, China; college of Animal Science and Technology, Southwest University, Chongqing 400716, China );PURIFICATION AND CHARACTERIZATION OF PHYTASE FROM ASPERGULLUS NIGER A 3214 AND A. NIGER N 14[J];Journal of Southwest Agricultural University;2006-04
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