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《Chinese Journal of Virology》 2003-03
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The LMP1 CTAR1 or LMP1 CTAR2 of Ep stein-Barr Virus Promotes Prolifer ation of Human HNE2 Cell Line

WANG Cheng-xing1,REN Wei2,LI Xiao-y an2,GU Huan-hua2,YI Wei2,WENG Xin -xian2,XIA Lin-qing2,DENG Xi-yun2,C AO Ya2 (1Oral and Maxillofacial Surgery, Xiangya Hospital,Central Southern University,Changsha 410078,China;2 Institute of Oncology,Xiangya Med ical College,Central Southern Uni versity,Changsha 410078,China)  
This paper was to investi gate the carcinogenic effect of differe nt regions of LMP1 gene of Epstein-Ba rr virus on proliferation of human nasopharyngeal carcinoma cell line HNE2The stable transfectant cell lines were established by introduc ing LPM1 cDNA,LMP1(1-185),LMP1(1-2 31)(LMP1 CTAR1),LMP1△187-351(LMP1 CTAR2) and the vector (pSG5) into HNE2-an EBV-negative cell line der ived from poorly differentiated na sopharyngeal carcinoma by electro porotionUsing these cell lines as models,the proliferative changes o f transfectants were measured in v itro by proliferative experiment,B rdU incorporation ratio assay,clon ing forming assay and transplantat ion in nude miceThe results showe d that the LMP1(1-231) and LMP1△187 -351 promoted the growth ability o f NPC HNE2 cells in vitroThe avera ge A ratio,BrdU incorporation rati o,cloning forming ability of the H NE2-LMP1(1-231) and HNE2-LMP1△187 -351 cells were higher than those of HNE2-pSG5 cell and HNE2 (P001)Incubation period and i nternal doubling time of transplanted tumor of HNE2-LMP1,HNE2-LMP1(1-231 ) and HNE2-LMP1△187-351 were marke dly shortenedIt suggests that LMP1 (1-231) and LMP1△187-351 can promo te the growth of NPC HNE2 cells,an d the LMP1 possibly plays an impor tant role in the development of NP C
【Fund】: 国家科技部"恶性肿瘤发生发展的基础研究(973)项目(G1998051201);; 国家自然科学基金杰出青年基金(3955022);; 美国中华医学基金(GMB96655);; 国家自然科学基金重点项目(3980410)
【CateGory Index】: R739.63
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【References】
Chinese Journal Full-text Database 1 Hits
1 LIU Lai-kui1,JIANG Hong-bing1,YIN Xin-min1,LI Xiao-qing2,YI Xin-zhu2.(1.Dept.of Oral Anatomy and Physiology,College of Stomatology,Nanjing Medical Science University,Nanjing 210029,China;2.Dept.of Oral Anatomy and Physiology,West China College of Stomatology,Sichuan University,Chengdu 610041,China);Regulation of PTHrP in Proliferation and Differentiation of Chondrocytes of Condyle in Fetal Mouse[J];West China Journal of Stomatology;2006-03
【Citations】
Chinese Journal Full-text Database 3 Hits
1 WANG Cheng-xing 2,DENG Xi-yun 1, LI Xiao-yan 1,GU Huan-hua 1,YI Wei 1,WENG Xin-xian 1,XIA Lin-qing 1,CAO Ya 1 (1.Cancer Research Institute of Xiangya Medical college,Central Southern University,Changsha 410078, China;2.Xiangya;The Proliferation of Nasopharyngeal Carcinoma Cell Line HNE2 after Transfected with Epstein-Barr Virus LMP1,CTAR1 and CTAR2[J];Chinese Journal of Virology;2001-04
2 WANG Cheng Xing,CAO Ya(Tumor Research Institute, Hunan Medical University ,Changsha 410078,China).;Signal Transduction Mediated by the Epstein Barr Virus encoded LMP-1[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1999-06
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Cheng-xing, LI Xiao-yan, CAO Ya, et al. (Cancer Research Institute of Hunan Medical University, Changsha 410078, P. R. China);Epstein-barr virus-encoded latent membrane protein 1 activates NF-kB via a mechanism involving TRAF2 in nasopharyngeal carcinoma[J];CHINESE JOURNAL OF CANCER;2000-06
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【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 Jiao Wei(Virology Laboratory. Guangxi Regional Hospital);ESTABLISHMENT OF A HUMANEPITHELIAL CELL LINE OF NASOPHARYNGEAL CARCINOMA─ CNE3 AND ITS BIOLOGICAL CHARACTERISTICS[J];ACTA ACADEMIAE MEDICINAE GUANGXI;1995-02
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