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《Chinese Journal of Virology》 2009-01
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Effect of the Gardenia Extracts-T9 on Viral Replication and IFN-γ mRNA in Herpes Simplex Virus Type-1 Infected Mice Brains

Shi Yu-jing1,Huang Yang1,Jiang Jing2,Guo Shan-shan1,Su Dan1,Zhao Ye1,Gao Ying-jie1,Cui Xiao-lan1*(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.Aerospace Center Hospital,Beijing,100049,China)  
RT-PCR was used to detect expression level of VP16 mRNA and IFN-γ mRNA in Herpes simplex virus type-1 infected mice brains at 4th day,7th day,10th day,14th day,21st day post infection and investigate the effects of the Gardenia extracts-T9 on viral replication and host immunity.The results showed that expression of VP16 mRNA in Gardenia extracts-T9 high dose and low dose group were both lower than that in virus control group at same time point.Relative VP16 mRNA expression in low dose group decreased at 21st day and relative VP16 mRNA expression in high dose group decreased continuously.Relative expression of IFN-γ mRNA in high dose and low dose groups were both higher than that in virus control group at all time point except the 4th day.IFN-γ mRNA in low dose group increased from the 4th day till the 14th day,and after the 14th day,the expression decreased slightly.Relative IFN-γ mRNA in high dose group maintained increasing from 4th day till 21st day.Base on these results,we conclude that Gardenia extracts-T9 might exert the inhibition effect of viral replication by upregulating expression of IFN-γ mRNA.
【Fund】: 国家自然科学基金面上项目(30672646);; 国家十一五科技支撑项目(2006BAI08B04-02)
【CateGory Index】: R285.5
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【Co-references】
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1 WANG Xue-hong et al (Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,The Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730050);Influence of Hypericum perforatum Extract on IFN-γ,TNF-α of Lung Tissue of Mice Infected with Influenza A Virus[J];Journal of Anhui Agricultural Sciences;2009-13
2 GUO Shan-shan~1,WANG Xiu-kun~1,ZHANG Yuan-yuan~2,GAO Ying-jie~1,HUANG Yang~1,ZHAO Ye~1,GONG Wen-feng~1,CUI Xiao-lan~(1*)(1.Institute of Chinese Meteria Medica,China Academy of Traditional Chinese Medicine,Beijing 100700,China;2.Chinese Institute of Materia Medica,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China);Effect of the Extract ZG from Gardenia on the Cell Adsorption of Herpes Simplex Virus Type 1(HSV-1)[J];Chinese Journal of Virology;2006-03
3 GUO Shan-shan,HUANG Yang,ZHAO Ye,GAO Ying-jie,GONG Wen-feng,CUI Xiao-lan*(Institute of Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China);Effect of Extracted ZG from Gardenia on Hep-2 Cell Membrane Post Infected with Parainfluenza Virus Type 1(PIV-1)[J];Chinese Journal of Virology;2007-05
4 Wang Zhijie Huang Tieniu Liu Yanwen et al Medical College of Wuhan University(430071)△Zhongnan University of Economics and Law;The Experimental Research of Antiherpes Simplex Virus Type-2 Effects of Total Saponins in the Root of Astragalus Membranaceus[J];Journal of Zhejiang College of Tcm;2001-05
5 Wang Zhijie,Huang Tieniu,Liu Yanwen Medical College of Wuhan University,Wuhan(430071);Pharmadynamics Analysis to Emodin in Rhizoma Polygoin Cuspidati and Total Saponins in the Root of Astragalus Membranaceus Used on Anti Herpus Virus[J];Journal of Zhejiang College of Tcm;2003-01
6 Wang Zhijie,Huang Qishan Virus Institute of Medical College in Wuhan University (430071);Pharmacodynamic Analysis on Resisting Type Ⅱ Human Herpes Virus with Resveratrol and Total Astragalin[J];Journal of Zhejiang College of Tcm;2005-06
7 ;盐酸小檗碱伍用炎琥宁治疗小儿病毒性肠炎发热型非发热型观察[J];Contemporary Medicine;2009-21
8 Han Gungyu Department of Natural Product Chemistry, Faculty of Pharmacy;STUDIES ON THE EFFECTIVE CONSTITUENTS OF ZHU-SHA-QI (POLYGONUM CILLINERVE (NAKAI)OHWI)[J];Academic Journal of Second Military Medical University;1985-01
9 LIU Sheng 2, CHEN Wan Sheng 1, QIAO Chuan Zhuo 1, ZHENG Shui Qing 1, ZENG Ming 1,ZHANG Han Ming 1, SONG Zhao Jun 1 (1. Department of Pharmacognosy, College of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2. Department;Antiviral action of Radix Isatidis and Folium Isatidis from different germplasm against influenza A virus[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;2000-03
10 LIANG Rong-Gan1, LIU Wei-Bing2, TANG Zu-Nian1, XU Qing1 1Department of Pharmacology, Guilin Medical College, 2College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China;Inhibition on respiratory syncytial virus in vitro by flavonoids extracted from the core of Litchi Chinensis[J];Journal of the Fourth Military Medical University;2006-20
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