Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Virology》 2012-02
Add to Favorite Get Latest Update

Genetic Variation and Pathogenicity Analysis of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Strain TJ in the Course of Attenuation

LENG-Xue1,LI Zhen-guang1,LI Xi-yu2,WANG Feng-xue1,ZHANG Xiu-hua1,WU-Hua1,2*(1.Division of Zoonosis,Institute of Special Economic Animal and Plant Sciences,Chinese Academy of Agricultural Science,Jilin 132109,China;2.Sinovet Beijing Biotechnology Co.,Ltd.,Beijing 100085,China)  
To develop an attenuated vaccine against the highly pathogenic porcine reproductive and respiratory syndrome(HP-PRRS) virus,the HP-PRRS virus strain TJ was attenuated by serial passages and plaque cloned every 5 to 10 passages in Marc-145 cells.Genetic variation and pathogenicity of HP-PRRSV strain TJ in the course of attenuation were analyzed.The results showed that the strain TJ sustained various sequence changes during the course of attenuation.Fifty-eight amino acids changes and a new continuous 120 amino acids deletion after the discontinuous 30 amino acids deletion(sites 481 and 533~561) occurred in strain TJ passages 140,and the position of 120 amino acids deletion was between 628 to 747 according to VR-2332.Animal test showed that the pathogenicity of strain TJ passages 20 was attenuated obviously,so we presume that genetic variation in nonstructural protein nsp2-nsp5,nsp7 and structural protein GP5 during the attenuation provides the molecular bases for the observed attenuated phenotype.
【Fund】: 科技部“十一五”科技支撑计划项目(2009BADB4B00)
【CateGory Index】: S852.65
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LENG Xue,WEN Yong-jun,QI Qiao-ling,LI Zhen-guang,WU Hua (Institute of Special Wild Economic Animal and Plant Sciences,CAAS,Jilin 132109,China);Isolation and Identification of the Very Virulent Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) TJ Strain[J];Journal of Jilin Agricultural University;2008-06
2 Guo Baoqing(Harbin Veterinary Research Institute,the Chinese Academy of Agricultural Sciences);Isolation and Identification of Porcine Reproductory and Respiratory Syndrome(PRRS) Virus from aborted fetuses suspected of PRRS[J];;1996-02
3 ZHOU Yan-jun,TIAN Zhi-jun,HAO Xiao-fang,AN Tong-qing,YU Hai,LI Guo-xin,CHEN Jin,PENG Jin-mei,TONG Guang-zhi (1.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;2.Harbin Veterinary Research Institute,CAAS,Harbin 150001,China);MOLECULAR CHARACTERIZATION OF HIGHLY PATHOGENIC PORCINE REPRODUCTIVE AND RESOIRATORY SYNDROME VIRUS(HuN4) AND ITS ATTENUATED STRAIN[J];Chinese Journal of Animal Infectious Diseases;2011-01
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 GAO Zhi-qiang,GUO Xin,CHEN Yan-hong,CHA Zhen-lin,YANG Han-chun rinary Medicine of Ministry of Agriculture,China Agricultural University,Beijing 100094,China);Molecular Characteristics of Complete Genome of a Chinese Porcine Reproductive and Respiratory Syndrome Virus HB-1(sh)/2002[J];Chinese Journal of Virology;2004-03
2 Yang Hanchun Huang Fangfang Guo Xin Gao Yun Li Hua Chen Sheng (College of Veterinary Medicine,Key laboratory of Preventive Medicine of Ministry of Agriculture, China Agricultural University,Beijing 100094);Sequencing of Genome of Porcine Reproductive and Respiratory Syndrome Virus isolate BJ-4[J];Journal of Agricultural Biotechnology;2001-03
3 GAO Zhi-qiang, GUO Xin, YANG Han-chun *, CHEN Yan-hong, ZHA Zhen-lin (Preventive Veterinary Medicine Key Laboratory of Ministry of Agriculture, China Agricultural University, Beijing 100094,China);Genomic Characterization of a Mutant of Porcine Reproductive and Respiratory Syndrome Virus with Deletion[J];Chinese Journal of Animal and Veterinary Sciences;2005-06
4 WANG Qiuquan,DENG Zhongping (DaAn Gene Diagnostic Center of Sun Yat-sen University, Guangdong, Guangzhou 510665, China);Development of real-time quantitative RT-PCR assay for detection of highly pathogenic porcine reproductive and respiratory syndrome virus[J];Journal of Molecular Diagnostics and Therapy;2009-01
5 Guo Baoqing(Harbin Veterinary Research Institute,the Chinese Academy of Agricultural Sciences);Isolation and Identification of Porcine Reproductory and Respiratory Syndrome(PRRS) Virus from aborted fetuses suspected of PRRS[J];;1996-02
6 TONG Guang-zhi ZHOU Yan-jun HAO Xiao-fang TIAN Zhi-jun QIU Hua-ji PENG Jin-mei AN Tong-qing (Division of Swine Infectious Diseases,National Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China);Identification and molecular epidemiology of the very virulent porcine reproductive and respiratory syndrome viruses emerged in China[J];Chinese Journal of Preventive Veterinary Medicine;2007-05
7 Qiu Huaji Guo Baoqing Tong Guangzhi  Liu Wenxing Yu Li (State Key Lab of Vet Biotechnology, Harbin Vet Res Inst, CAAS, Harbin 150001);Genotyping of Porcine Reproductive and Respiratory Syndrome Virus Strain CH 1a Isolated in China by RT PCR and Indirect Immunofluorescence Test[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1998-02
8 Zhao DemingWang AipingGan MenhouZhang ZhongzhiHe WeiyongSun ZhongqiLiu Zhencheng (China Agricultural University,Beijing 100094);Studies on Pathological Characteristics of Porcine Reproductive and Respiratory Syndrome(PRRS)[J];CHINESE JOURNAL OF VETERINARY MEDICINE;1996-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved