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《CHINESE JOURNAL OF VIROLOGY》 1994-02
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IDENTIFICATION OF THE GENOTYPE OF VP4 AND VP7 OFROTAVIRUSES CIRCULATING IN SEVERAL AREASOF CHINA

Yuan Lijuan1 Qian Yuan1 Liu Jun1 Zhang You1 Xiong Zaohui1 Guan Dehua2 Liu Changaing3 Deng Linfei3 Guo Yuru4 Xie Ruonan5 Zhang Zhenhua1 Zhao Huixin1 Wang Zhiliang11. Capital Institute of Pediatrics ,Beijing Municipal Laboratory of In fection and Immunity,B  
tool specimens were collected form children with diarrhea in the autumn and winter of 1991 form Beijing ,ChangchunmXingtai and Shantou,located in the north,northeast and south of China.out of 5540 specimens collected,334 (60.7% ) were positive for rotaviruses as deter-mined by polyacrylamide gel electrophoresis (PAGE).VP4 and VP7 genotypes of the viruseswere identified by Northern blot hybridization analysis using 32P labeled VP4 and VP7genotype specific cDNA clones as probes.The data indicated that most of the rotavirusescirculating in this country belong to the genotype combination of VP4 1A and VP7Thirty percent of the virusens belong to VP4 1B and VP7 2.Viruses with VP4 1A and VP7 3 were only found in specimens from Beijing.Rotavirus with VP7 9 was detected from one stool specimen collected in Beijing.Coinfections by rotaviruses with different VP4 and VP7genotypes were identified by Northern blot hybridization as well as by nested polymerase chainreaction(PCR).
【CateGory Index】: R373.24
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5 Yuan Lijuan1 Qian Yuan1 Liu Jun1 Zhang You1 Xiong Zaohui1 Guan Dehua2 Liu Changaing3 Deng Linfei3 Guo Yuru4 Xie Ruonan5 Zhang Zhenhua1 Zhao Huixin1 Wang Zhiliang11. Capital Institute of Pediatrics ,Beijing Municipal Laboratory of In fection and Immunity,B;IDENTIFICATION OF THE GENOTYPE OF VP4 AND VP7 OFROTAVIRUSES CIRCULATING IN SEVERAL AREASOF CHINA[J];病毒学报;1994-02
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