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《CHINESE JOURNAL OF VIROLOGY》 1998-03
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STUDIES ON in Vitro ANTI-INFLUENZA A VIRUS ACTIVITY OF ANTISENSE PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES

Chen Zhongbin Wang Shengqi Zhu Baozhen Sun Zhixian (Department of Biochemistry and Molecular Biology,Institute of Radiation Medicine,Beijing 100850)  
Synthetic oligonucleotides represent the promising antiviral therapeutic agents.For developing antisense oligonucleotides as potential antiviral therapeutic agents against influenza A virus, phosphorothioate oligodeoxynucleotides(PS-ODN)targeted to part of the 3′and 5′end sequences which are common to the eight RNAs of type A influenza virus were synthesized.Antiviral activity of these PS-ODNs was evaluated by hemagglutination titer assay using cultured MDCK infected by A/JingFang/86-1(H 1N 1).Oligonucleotides,IV4 #,complementary to 5′ terminal conserved sequence exhibited the most potent antiviral activity with reduced hemagglutination titer about 50% at the concentration of 1μmol/L,and over 70% at 10μmol/L.The antiviral activity of IV4 # was in a dose-and sequence-dependent manner.Incorporation of hydrocarbon chains onto IV4 # showed no increased inhibitory activity.In the presence of lipofectin,IV4 # and its hydrocarbon chain linked analog exhibited markedly increased antiviral activity with inhibitory activity from 56.2% to 75% and from 30% to 57.5% respectively at the concentration of 1μmol/L.IV4 # may provide a candidate antisense oligonucleotides for the treatment of human influenza.
【Fund】: 国家“863”计划基金 总后卫生部“9.5”重点项目基金
【CateGory Index】: R373.13,
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