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《CHINESE JOURNAL OF VIROLOGY》 1999-01
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EXPRESSION OF TYPE G1 VP7 FROM A GROUP A ROTAVIRUS FIELD STRAIN IN BACULOVIRUS SYSTEM

He Xiangjun Qian Yuan Jing Yu Li Guohua (Capital Institute of Pediatrics, Beijing Municipal Laboratory of Infection and Immunity, Beijing, 100020)  
Reverse transcription and polymerase chain reaction(RT PCR) were used to amplify the coding region of VP7 of a G1 type group A rotavirus field strain CR188 circulated in Beijing. The amplified cDNA was cloned into the downstream of the polyhedrin promoter of transfer Vector pBacPAK8. Sequence analysis showed 95.7% homology of the deduced amino acid between VP7 of CR188 and that of G1 prototype Wa. Insect cells were cotransfected with plasmid pBacPAK8 CR188 and Bsu36I digested BacPAK6 viral DNA to produce recombinant baculoviruses by homologous recombination. Recombinant viruses containing CR188 VP7 gene were identified by PCR using the forward primer on the polyhedrin promoter and the downstream primer of VP7. Specific VP7s with four different molecular weights were detected by Western blot using hyperimmune serum against Wa when the VP7 of CR188 was expressed in insect cells.
【Fund】: 国家自然科学基金 卫生部科研基金 北京市自然科学基金
【CateGory Index】: Q939.403
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【References】
Chinese Journal Full-text Database 3 Hits
1 LI Yuxian;GUAN Lili;YANG Jing;LIU Xiuming;YANG He;ZHOU Yonggang;WANG Hongyu;LI Haiyan;LI Xiaokun;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University;Traditional Chinese Medicine Department,Jilin Agricultural Science and Technology College;College of Traditional Chinese Medicine,Jilin Agricultural University;Wenzhou University;;Expression and immunogenicity analysis of the VP7 gene of rotavirus in transgenic Arabidopsis thaliana[J];黑龙江畜牧兽医;2017-09
2 LI Yu-xian;GUAN Li-li;ZHOU Yong-gang;LIU Xiu-ming;YANG Jing;GUO Yong-xin;YANG He;WANG Hong-yu;LI Hai-yan;LI Xiao-kun;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University;Traditional Chinese Medicine Department,Jilin Agricultural Science and Technology College;College of Traditional Chinese Medicine,Jilin Agricultural University;Wenzhou University;;Expression and immunogenicity analysis of VP7-CTB fusion protein in transgenic Arabidopsis thaliana seeds[J];中国兽医科学;2017-04
3 SHEN Wen-tao,ZHOU Peng (State Key Laboratory of Tropical Cro p Biotechnology,Institute of Tropical Biology Sciences,China Academy of Tropical Agricultu ral Science,Haikou571101,Hainan,China);Expression of VP7 of Rotavirus Outer Capsid Protein in Transgenic Tomato[J];生命科学研究;2004-03
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 HE Jin sheng,WANG Jian wei,WEN Le ying,XU Shu sheng,QU Jian guo,JIANG Hui ying,HONG Tao (Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China);The Systemic and Mucosal Immune Responses Can Be Induced Effectively by a Recombinant Adenovirus (Ad) Expressing Main Neutralizing Antigen VP7 of Group A Rotavirus[J];病毒学报;2001-02
2 YUAN Li\|Yong *\ LIU Yong\ LI Chun\|Hong\ SUN Mao\|Sheng\ DAI Chang\|Bai (Institute of Medical Biology,CAMS \%&\% PUMC,Kunming 650118,China);Expression in Escherichia coli and Immunogenicity of Rotavirus VP7[J];生物工程学报;2001-02
3 He Xiangjun Qian Yuan Jing Yu Li Guohua (Capital Institute of Pediatrics, Beijing Municipal Laboratory of Infection and Immunity, Beijing, 100020);EXPRESSION OF TYPE G1 VP7 FROM A GROUP A ROTAVIRUS FIELD STRAIN IN BACULOVIRUS SYSTEM[J];病毒学报;1999-01
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 WANG Lei et al(Qingdao University of Science and Technology,Qingdao,Shandong 266042);Research Progress of Tomato Gene Engineering[J];安徽农业科学;2013-07
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