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《CHINESE JOURNAL OF VIROLOGY》 1999-02
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CHARACTERIZATION OF φMMR1, A NEWLY ISOLATED NARROW HOST-RANGE LYTIC BACTERIOPHAGE OF AMYCOLATOPSIS MEDITERRANEI U119

Li Weiquan 1 Yao Heming 2 Shen Meijuan 1 jin Meilei 3 Zhao Guoping 3 Chiao Ruishen 1  
Bacteriophage φMMR1 was isolated from a lysed industrial fermentation culture of Amycolatopsis mediterranei U119. φMMR1 is likely to be lytic phage, because more than 90% of the plaques were clear and no lysogens, immune to phage infection were obtained. φ MMR1 could form plaques only on laboratory strains of A. mediterranei among 11 tested strains. The host range of the phage is so narrow that it failed to infect the very closely related species, A. orientalis, A. rugosus, A. sulphreaus and A. methanolica . The conditions of propagation and storage of φMMR1, such as pH, temperature, divalent cations and organic solvents, have been studied in detail. The virion structure observed under electron microscopy is similar to that of most known Streptomycetes' phage. It belongs to the B1 group of Styloviridae. Rabbit antiserum directed against φMMR1 was prepared and a onestep growth curve was obtained.Restriction enzyme analysis of the genomic DNA indicated a unique pattern different from those of other known Actinophages. The phage genome is linear, double-stranded and no cohesive ends could be tested. The genome is ca. 59.6 kb in size. The G+ C content of φMMR1 DNA was determined as 67% by HPLC. The proteins of the phage capsid were analyzed by SDS-PAGE. Purified phage DNA was able to transfect the standard laboratory strain A. mediterranei U-32 through electroporation.
【Fund】: 中国自然科学基金
【CateGory Index】: Q939.48
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