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《Acta Zoologica Sinica》 1978-02
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CULTIVATION OF TISSUES AND CELLS FROM AMPHIBIA

Wu ZHENGAN(Department of Cell Biology, Institute of Zoology, Acadcmia Sinica)  
1.The tongue,urinary bladder,pericardial membrane,lung etc.,altogether 13 kinds of tissues were isolated from the common toad (Bufo bufo gargarizans) and cultivated,the tongue of frog (Bana plancyi) was used for comparison.Observations were made on cell growth and multiplication as well as on other behavior.2.Then the toad kidey and lung tissues as well as frog kidney tissue were selected for monolayer cell cultivation.It was demonstrated that the two commonly used culture media (GLPY and modified Eagle medium) and the method for mono-layer cell culture could be well adapted for amphibian cell culture.3.To isolate cells from esplants for cultivation,the writer found that the use of 0.5% trypsin mixed with 0,01% EDTA and incubated at 25±1℃for a short period of digestion proved to be more satisfactory as compared with the methods used by earlier workers.This new method may be considered as a definite improvement.4.The cytological observations showed that although the epithelium tissues and cells from different sources appeared on the whole quite similar,yet the differences in cell growth and behavior as well as the capacity for multiplication were apparent.In maintaining the original characteristics of the defferent kinds of cells,it appeared that they manifested a certain degree of constancy and irreversibility.Thus it offers a great possibility to use such characteristics for the hybridization study of somatic cells.In this respect,it is to be noted that the ciliary movement of lung tissue from toad,after five months of culture in vitro was still maintained as before without showing least tendency of weakening.The cells of toad's urinary bladder,while migrating outward from the explant to form an outgrowth,showed very little activity of cellular multiplication.It thus appears that cell movement may be considered as a kind of manifestation for cellular differentiation,because it showed a tendency to inhibit cell division.It was further observed that cell division showed a certain degree of synchronization.This may mean that the rate and onset of cell division are determined by cytoplasmic and environmental factors.For example,chick embryonic extract can exert an obvious effect on cell multiplication.The discovery of synchronization as well as non-synchronization of the dinucleated cells and the multipolar divisions of some cells offers problems for further study,especially in view of the fact that a great deal of attention has been paid in recent years to the cytological studies of cell fusion etc.5.In the course of cell division,some abnormalities of metaphase figures,such as chromosome bridge formations were found.This is apparently due to the crossing-over within the heterozygous inverted regions of the chromosomes.While it may be a common occurence in meiosis but in mitosis it is extremely rare.The significance of the observations mentioned was discussed.
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【References】
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1 Wen Changxiang Department of Biology, Bengbu Medical College, Bengbu, Anhui;CHROMOSOMAL KARYOTYPE OF RANA LIMNOCHARIS BOIE[J];Journal of Bengbu Medical College;1982-01
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3 WU ZHENGAN (Department of Cell Biology, Institute of Zoology, Academia Sinica);CHROMOSOMAL STUDIES ON CULTURED AMPHIBIAN SOMATIC CELLS IN VITRO[J];Acta Zoologica Sinica;1978-02
4 WU ZHeNGAn YANG HUIYI(Department of Cell Biology, Institute of Zoology, Academia Scinica);KARYOTYPE ANALYSIS OF FROGS AND TOADS BY THE METHOD OF LYMPHOCYTE CULTURE[J];Acta Zoologica Sinica;1980-01
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 WU ZHENGAN (Department of Cell Biology, Institute of Zoology, Academia Sinica);CHROMOSOMAL STUDIES ON CULTURED AMPHIBIAN SOMATIC CELLS IN VITRO[J];Acta Zoologica Sinica;1978-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU ZHeNGAn YANG HUIYI(Department of Cell Biology, Institute of Zoology, Academia Scinica);KARYOTYPE ANALYSIS OF FROGS AND TOADS BY THE METHOD OF LYMPHOCYTE CULTURE[J];Acta Zoologica Sinica;1980-01
2 WU ZHENGAN(Institute of Developmental Biology, Academia Sinica, Beijing);SOMATIC CHROMOSOME OF HASHIMA-FROG[J];Acta Zoologica Sinica;1982-01
3 YANG HUIYI(Institute of Developmental Biology,Academia Sinica);THE KARYOTYPE OF THE TOAD BUFO RADDEI STRAUCH[J];Acta Zoologica Sinica;1982-01
4 Wu Zhengan Yin Jianwen(Institute of Developmental Biology, Academia Sinica, Beijing);KARYOTYPIC AND C-BANDING ANALYSIS OF RANA AMURENSIS[J];Acta Zoologica Sinica;1983-01
5 LIANG GUI-XIA MAO MING-TING(Department of Biology, Northwestern Normal University);CHANGES OF CHROMOSOMES DURING EARLY DEVELOPMENT OF THE EMBRYOS OBTAINED BY NUCLEAR TRANSPLANTATION BETWEEN BUFO BUFO GARGARIZANS AND B. RADDEI[J];Acta Zoologica Sinica;1991-03
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7 Li Shu-shen Wang Yin-xiang Li Chong-yun Wang Rui-fang Liu Guang-zuo (Kunming Institute of Zoology, Academia Sinica.);A comparative investigation of the karyotypes from Four Amphibian species[J];Zoological Research;1981-01
8 Chang Chuming Wang Aihua Guo Ping ( Wenzhou Medical College Zhejiang );THE KARYOTYPE OF THE FROG (RANA TIGRINA RUGULOSA)[J];Zoological Research;1984-S2
9 Gao Jian-min (The Research Laboratory of Experimental Embryology, Department of Biology, Fujian Teachers University);The Karyotype of Rana guentheri Boulenger[J];Journal of Fujian Normal University(Natural Science Edition);1984-01
10 DONG Zi-mei,SUN Xiao-ling,GUO Pei-jun(Puyang Vacation-technical College,Puyang 457000,China);Karyotype,Ag-NORs and Sex Determination of Bufo raddei from Henan[J];Journal of Henan Normal University(Natural Science);2009-01
【Co-references】
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1 WAN Quan 1,LIU En sheng 1,SHENG De ling 2,TANG Hui qing 1,XUE Ming 1,LI Yan he 1 (1 Department of Fishery,Anhui Agricultural University,Hefei 230036; 2 Fisheries Station of Anhui Province);Analysis on Karyotype of Leiocassis longirostris Günther[J];Journal of Anhui Agricultural University;2002-02
2 WU ZHENGAN (Department of Cell Biology, Institute of Zoology, Academia Sinica);CHROMOSOMAL STUDIES ON CULTURED AMPHIBIAN SOMATIC CELLS IN VITRO[J];Acta Zoologica Sinica;1978-02
3 WU ZHeNGAn YANG HUIYI(Department of Cell Biology, Institute of Zoology, Academia Scinica);KARYOTYPE ANALYSIS OF FROGS AND TOADS BY THE METHOD OF LYMPHOCYTE CULTURE[J];Acta Zoologica Sinica;1980-01
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5 WU ZHENGAN YANG HUIYI(Institute of Developmental Biology,Academia Sinica,Beijing);THE KARYOTYPE OF Kaloula borealis[J];Acta Zoologica Sinica;1981-01
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7 YANG HUIYI(Institute of Developmental Biology,Academia Sinica);THE KARYOTYPE OF THE TOAD BUFO RADDEI STRAUCH[J];Acta Zoologica Sinica;1982-01
8 Wu Zhengan Yin Jianwen(Institute of Developmental Biology, Academia Sinica, Beijing);KARYOTYPIC AND C-BANDING ANALYSIS OF RANA AMURENSIS[J];Acta Zoologica Sinica;1983-01
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10 ;AN ANALYSIS OF THE KARYOTYPE OF SPOT-LEGGED TREE FROG (RHACOPHORUS LEUCOMYSTAX)[J];Acta Zoologica Sinica;1983-03
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