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《Acta Zoologica Sinica》 1989-04
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STUDIES OF HETEROFERTILIZATION IN VITRO BETWEEN GIANT PANDA AND GOLDEN HAMSTER

CHEN DAYUAN SHI QIXIAN SONG XIANGFEN ZHAO XUEKUN DUAN CHONGWEN(Lab. for Reprod. Biol., Inst. of Zool. Academia Sinica, Beijing)HE GUANGXIN YE ZHIYONG ZHANG ANJU(Chengdu Research Base of Giant Panda Breeding)FENG WENHE (Dept. of Biol., Sichuan University, Chengdu)  
The golden hamster eggs could be fertilized by giant panda sperm capacitated in vitro. The second polar body was released after spermpenetration and the ooplasm of such fertilized egg was found to be able to induce pronucleus formation. The male pronucleus formed by ho-mofertilization usually starts decondension from the periphery toward to the center of nucleus, while in heterofertilization the pronuclear decon densation process is irregular and imcomplete resulting in relative smaller size.Cytochalasin B treatment after sperm penetration prevents the male pronucleus migration from the egg cortex toward the center. It is(?) the efore, suggested that the male pronucleus migration of giant panda is also controlled by the microfilaments of the heterogeneous ooplasm.
【Key Words】： : Heterofertilization In vitro Pronucleus Microfilament Giant panda.
【Fund】： 国家重大科研项目;; 美国纽约洛氏基金会
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