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《Acta Zoologica Sinica》 1989-04
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POTENTIATION OF MITOGENIC RESPONSE BY EXTRACTS OF THE SWIFTLET'S (APUS) NEST COLLECTED FROM HUAI-JI

Y.C.KONG, S.W.TSAO, M.E.SONG, M.H.NG.(Department of Biochemistry, and Anatomy, The Chinese Univcrsity of Hong Kong, Shatin, Hong Kong; and Department of Microbiology, University oj Hong Kong, Poklulam Road, Hong Kong)LIN ZHEFU(Department of Biology, Guangzhou, Guangdong, PRC)  
S. An epidermal growth factor (EGF)-like activity (EGF-2) was detected and partially purified by Bio-Gel P-10 column from aqueous extract of the cave swiftlet's (Apus affinis Subfur-catus Blyth) nest, collected from Huai-Ji county, Guangdong Province.2. The partially purified (EGF)-Iike activity (EGF-2) was able to (a) generate competitivebinding curves parallel to the mouse EGF standard curves in radioreceptor assay and (b) stimulate thymidine incorporation in quiescent culture of 3T3 fibroblasts, the latter activity can not be suppressed by mouse EGF antibody.3. A protein fraction (S-200 11111111111111111+22222222222222), partially purified by Sephacry111111111111-200 Superfine chromatogr-aphy, was able to (a) produce mitogenic response in human lymphocyte culture and to (b) potentiate mitogenic response in Con A transformed lymphocyte culture.
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10 Wang Lingli1,Huang Song2,Jiang Dongxu3,Li Yuanbin3,Wu Guohong1,Lai Xiaoping2,3(1.School of Chinese Pharmacy,Guangzhou University of Chinese Medicine,Guangzhou 510006,China; 2.Research Center of New Drug Development,Guangzhou University of Chinese Medicine, Guangzhou 510006,China; 3.Dongguan Research Institute of Mathematical Engineering of Guangzhou University of Chinese Medicine, Dongguan 523808,China);Identification and Pharmacological Progress on Cubilose[J];Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology;2013-01
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